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69522 | T7•Tag® Monoclonal Antibody

69522
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      69522-3
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          Glass bottle 50 μg
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          69522-4
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              Glass bottle 250 μg
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              Description
              OverviewThe T7•Tag® Antibody is a mouse monoclonal antibody (IgG2b) directed against the 11 amino acid gene 10 leader peptide expressed by many of the pET translation vectors as well as pSCREEN™ and pRSET vectors. Because the peptide is the natural amino terminal end of the T7 major capsid protein, the antibody also recognizes T7 bacteriophage (e.g., the T7Select™ Vectors). For increased versatility and sensitivity, this antibody is also available labeled with biotin and as a direct conjugate with alkaline phosphatase or horseradish peroxidase.
              SpecificityT7•Tag peptide MetAlaSerMetThrGlyGlyGlnGlnMetGly; N-terminal, C-terminal or internal
              Species/IsotypeMouse monoclonal IgG2b
              Cross-reactivityNegligible with bacterial, insect or mammalian cell lysates
              Sensitivity1–10 ng (Western blot developed with chromogenic substrates)
              < 1 ng (AP or HRP conjugate developed with chemiluminescent substrates, or Biotin conjugate developed with Streptavidin AP and chromogenic substrates). Also suitable for immunoprecipitation and immunohistochemistry (1–3)
              FormStabilized solution of antibody in 50% glycerol; includes Positive Control Extract containing 31.1 kDa target protein
              Working dilution1:5,000–1:10,000
              Catalogue Number69522
              Brand Family Novagen®
              References
              References1. Krek, W., et al. 1994. Cell 78,, 161.

              2. Roberts, S.G.E. and Green, M. (1994) Nature 371, 717–720.

              3. Joneson, T., McDonough, M., Bar-Sag, D., and Van Aelst, L. (1996) Science 274, 1374–1376.
              Product Information
              Components
              Applications
              Biological Information
              Physicochemical Information
              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              Safety Information
              Product Usage Statements
              Storage and Shipping Information
              Ship Code Shipped with Blue Ice or with Dry Ice
              Toxicity Multiple Toxicity Values, refer to MSDS
              Storage -20°C
              Avoid freeze/thaw Avoid freeze/thaw
              Do not freeze Ok to freeze
              Packaging Information
              Transport Information
              Supplemental Information
              Specifications

              Documentation

              SDS

              Title

              Safety Data Sheet (SDS) 

              Certificates of Analysis

              TitleLot Number
              69522

              References

              Reference overview
              1. Krek, W., et al. 1994. Cell 78,, 161.

              2. Roberts, S.G.E. and Green, M. (1994) Nature 371, 717–720.

              3. Joneson, T., McDonough, M., Bar-Sag, D., and Van Aelst, L. (1996) Science 274, 1374–1376.

              Citations

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            • Uchimura, Y., et al. 2006. Journal of Biological Chemistry 281, 23180-23190.
            • Zhang, F., et al. 2006. Journal of Biological Chemistry 281, 30036.
            • Allemand, E., et al. 2005. Proceedings of the National Academy of Sciences (USA) 102, 3605.
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            • Barboric, M., et al. 2005. European Molecular Biology Organization Journal 24, 4291.
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            • Kuroda T.S., and Fukuda, M., 2005. Journal of Biological Chemistry 280, 28015.
            • Lin, Y., et al. 2005. Journal of Biological Chemistry 280, 12799.
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            • Papandreou, I., et al. 2005. Cancer Research 65, 3171.
            • Qiu, R., et al. 2005. Journal of Biological Chemistry 280, 16695.
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            • Yanagi, K., et al. 2005. Journal of Biological Chemistry 280, 19689.
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            • Ball, J.R., et al. 2004. RNA 10, 19.
            • Fukuda, M. and Kuroda T.S., 2004. Journal of Cell Science 117, 583.
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            • User Protocols

              Title
              TB015 T7•Tag<sup>®</sup> Antibody

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