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17-131 | Src Assay Kit

17-131
1 kit  
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      Overview

      Replacement Information

      Key Specifications Table

      Key ApplicationsAnalytes AvailableDetection Methods
      KA Src Radioactive
      Description
      Catalogue Number17-131
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionSrc Assay Kit
      References
      Product Information
      Components
      • Src Kinase Reaction Buffer (Cat.# 20-121)
      • Src Substrate Peptide (Cat.# 12-140)
      • Src Manganese/ATP Cocktail (Cat.# 20-110)
      • P81 Phosphocellulose Squares (Cat.# 20-134)
      Detection methodRadioactive
      Applications
      ApplicationThe radioactive Src Assay Kit is designed to measure the phosphotransferase activity of Src kinase in immunoprecipitates & column fractions.
      Key Applications
      • Kinase Assay
      Biological Information
      Analytes Available
      • Src
      Entrez Gene Number
      Entrez Gene SummaryThis gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
      Gene Symbol
      • SRC
      • ASV
      • c-src
      • p60-Src
      • SRC1
      • c-SRC
      • pp60c-src
      UniProt Number
      UniProt SummarySIZE: 536 amino acids; 59835 Da
      SUBUNIT: Interacts with DDEF1/ASAP1 via its SH3 domain. Interacts with CCPG1 (By similarity). Interacts with CDCP1, PELP1, TGFB1I1 and TOM1L2.
      PTM: Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. The phosphorylated tail interacts with the SH2 domain thereby repressing kinase activity.
      SIMILARITY: SwissProt: P12931 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. & Contains 1 protein kinase domain. & Contains 1 SH2 domain. & Contains 1 SH3 domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evalauted by phosphorylation of Src Substrate Peptide
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size1 kit
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      Src Assay Kit - 2383137 2383137
      Src Assay Kit - 2436392 2436392
      Src Assay Kit - 16133 16133
      Src Assay Kit - 17411 17411
      Src Assay Kit - 18015 18015
      Src Assay Kit - 18015a 18015a
      Src Assay Kit - 18644 18644
      Src Assay Kit - 19513 19513
      Src Assay Kit - 2043480 2043480
      Src Assay Kit - 20580 20580
      Src Assay Kit - 2064531 2064531
      Src Assay Kit - 20837 20837
      Src Assay Kit - 21392 21392
      Src Assay Kit - 2166053 2166053
      Src Assay Kit - 22971 22971
      Src Assay Kit - 2297190 2297190
      Src Assay Kit - 23386 23386
      Src Assay Kit - 27390 27390
      Src Assay Kit - 27390A 27390A
      Src Assay Kit - 32658 32658
      Src Assay Kit - DAM1400015 DAM1400015
      Src Assay Kit - DAM1668784 DAM1668784
      Src Assay Kit - JBC1940031 JBC1940031
      Src Assay Kit -2512495 2512495

      References

      Reference overviewApplicationPub Med ID
      Caveosomal Oxidative Stress Causes Src-p21ras Activation and Lysine 63 TRAF6 Protein Polyubiquitination in Iron-induced M1 Hepatic Macrophage Activation.
      Shuping Zhong,Jun Xu,Peggy Li,Hidekazu Tsukamoto
      The Journal of biological chemistry 287 2012

      Show Abstract
      22829592 22829592
      New role for the protein tyrosine phosphatase DEP-1 in Akt activation and endothelial cell survival.
      Catherine Chabot,Kathleen Spring,Jean-Philippe Gratton,Mounib Elchebly,Isabelle Royal
      Molecular and cellular biology 29 2009

      Show Abstract Full Text Article
      18936167 18936167
      Srcasm modulates EGF and Src-kinase signaling in keratinocytes.
      Weijie Li, Christine Marshall, Lijuan Mei, Leonard Dzubow, Chrysalene Schmults, Michael Dans, John Seykora
      The Journal of biological chemistry 280 6036-46 2005

      Show Abstract
      Immunoprecipitation15579470 15579470
      c-Src-dependent activation of the epidermal growth factor receptor and mitogen-activated protein kinase pathway by arsenic. Role in carcinogenesis.
      Simeonova, Petia P, et al.
      J. Biol. Chem., 277: 2945-50 (2002) 2002

      11723127 11723127
      G alpha(i) and G alpha(o) are target proteins of reactive oxygen species
      Nishida, M., et al
      Nature, 408:492-5 (2000) 2000

      Kinase Assay11100733 11100733
      Growth hormone stimulates the formation of a multiprotein signaling complex involving p130(Cas) and CrkII. Resultant activation of c-Jun N-terminal kinase/stress-activated protein kinase (JNK/SAPK)
      Zhu, T., et al
      J Biol Chem, 273:33864-75 (1998) 1998

      Kinase Assay9837978 9837978

      Related Products & Applications

      Kit Component

      Catalog Number Description  
      12-140 Src Substrate Peptide Show Pricing & Availability
      20-110 Src Manganese/ATP Cocktail Show Pricing & Availability
      20-121 Src Kinase Reaction Buffer Show Pricing & Availability
      20-134 P81 Phosphocellulose Squares Show Pricing & Availability

      Categories

      Life Science Research > Protein Detection and Quantification > Activity Assays > Kinase Assays