A part of MilliporeSigma

03-0099-01 | Singulex Erenna SMC Total-AKT1 Immunoassay Kit

96-well strip plate  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
Request Pricing
Limited AvailabilityLimited Availability
Limited Quantities Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service


      Contact Customer Service

      Click To Print This Page


      Replacement Information

      Key Specifications Table

      Species ReactivityKey Applications
      H, R, M Single Molecule Counting
      Catalogue Number03-0099-01
      DescriptionSingulex Erenna SMC Total-AKT1 Immunoassay Kit
      OverviewThe Singulex® Erenna® SMC® Immunoassay Kit contains all reagents required to perform assays on the Erenna® Immunoassay System. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The Erenna® Immunoassay System detects analytes using Single Molecule Counting (SMC®) technology.
      Background InformationThe serine-threonine protein kinase AKT family is the primary downstream mediator of the phosphatidylinositol-3-kinase (PI3K) signaling pathway. Members of the family (AKT1, AKT2, and AKT3) require two separate kinases for complete activation. AKT1 phosphorylates mTOR, whose downstream targets include other kinases, cell cycle regulators and transcription factors that are essential for many cell proliferation and survival processes. AKT1 also up-regulates matrix metalloproteinases and is thus implicated in tumor progression. AKT1 and other members of the AKT family have been an important focus in therapeutic development with their broad effects on the PI3K pathway.
      Product Information
      • TOTAL AKT1 Coated Beads
      • Sample Diluent
      • TOTAL AKT1 Detection Antibody
      • Assay Buffer
      • AKT1 Standard
      • AKT1 Detection Antibody Buffer
      • 10X Wash Buffer
      • Elution Buffer B
      • Buffer D
      • Erenna TOTAL AKT1 Immunoassay Kit Instructions
      Precision, %
      • Low-end CV% Average: 10%
      • Low-end CV% Range: 2 - 15%
      ApplicationThe sensitivity Singulex of Single Molecule Counting (SMC) gives Erenna SMC Total AKT-1 Immunoassay Kit unparalleled performance in the quantification of Total AKT-1 in cell lysates.
      Key Applications
      • Single Molecule Counting
      Biological Information
      Species Reactivity
      • Human
      • Rat
      • Mouse
      • Minimum of 10 samples spiked with acceptable recovery between 80-120%.
      Physicochemical Information
      Linearity of DilutionMinimum of 10 samples spiked and diluted (at least 3 dilutions) with acceptable recovery between 80-120%.
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size96-well strip plate
      Transport Information
      Supplemental Information

      Related Products & Applications

      Related Products

      Catalog Number Description  
      03-0100-01 Singulex Erenna SMC Human Phospho-AKT1 (pSer473)
      Show Pricing & Availability


      Life Science Research > Protein Detection and Quantification > Immunoassays > Enzyme-linked Immunosorbent Assay (ELISA) > Complete ELISA Kits
      Life Science Research > Antibodies and Assays > Immunoassays > Enzyme-linked Immunosorbent Assay (ELISA) > Complete ELISA Kits