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AQ300D | Sheep Anti-Mouse Ig Antibody, F(ab')2, Digoxigenin conjugate

200 µg  
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      Replacement Information
      Catalogue NumberAQ300D
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionSheep Anti-Mouse Ig Antibody, F(ab')2, Digoxigenin conjugate
      OverviewThe reagent is an anti-mouse Ig antibody, F(ab')2 fragment from sheep. The F(ab')2 fragments are obtained by pepsin digestion and conjugated with digoxigenin-N-hydroxy-succinimide ester and stabilized in buffer.

      PURIFICATION: The sheep-lgG is purified by ion exchange chromatography and the specific IgG was isolated by immunosorption. Cross-reacting antibodies were removed by further affinity chromatography using human-Ig and bovine Ig coupled to a solid phase.
      Product Information
      PresentationLyophilized from PBS, pH 7.4 containing 0.5% BSA and 0.1% sodium azide. Reconstitute with 1 mL of sterile distilled water. Concentration after reconstitution is 200 μg/mL.
      ApplicationDetect Mouse Ig using this Sheep anti-Mouse Ig Antibody, F(ab')2, Digoxigenin conjugate validated for use in ELISA, IH & WB.
      Key Applications
      • ELISA
      • Immunohistochemistry
      • Western Blotting
      Application NotesThis highly specific reagent allows a reliable and sensitive detection of mouse- Ig, especially also in such situations where interference of immunoglobulins of other species than mouse might occur. The digoxigenin labeled antibody can be used analogous to the biotinylated antibody in a number of immunological detection systems (Savage 1986) such as ELISA (Baumgarten 1986; Kendal et al. 1983), immunohistochemistry, respectively immunocytochemistry (Volson 1984; Gonatas et al. 1987), immunoblotting (Gabridge et al. 1986; Ogata et al. 1983) and screening of monoclonal antibodies, whereby the various anti-digoxigenin conjugates allow a high flexibility.


      Immunohistochemistry: 20-40 μg/mL

      ELISA: 0.05-2 μg/mL

      Immunoblotting: 10-20 μg/mL

      The concentrated stock solution (200 μg/mL) should be diluted with PBS, pH 7.4 containing bovine serum albumin (BSA) 0.5% (w/v).

      Optimal working dilutions must be determined by the end user.
      Biological Information
      SpecificityThe reaction profile of the antibody was examined in an ELISA sandwich system, where the same antibody was used for coating and, labeled with digoxigenin, also for detection. According to this assay, the antibody reacts with mouse Ig classes IgA, IgG, IgM and the mouse IgG subclasses IgG1, IgG2a, IgG2b, IgG3 as well as with the l- and k- chain. Cross-reactivity was determined with normal sera, diluted 1:10, of various animal species. The antibody shows no cross-reactivity (<0.01%) with fetal calf serum (FCS) and normal serum from bovine, rabbit, goat, horse, and human. Weak reaction can be detected with normal serum from guinea pig (<0.1%). Cross-reactivity can be detected with normal serum from rat (<10%).
      Species Reactivity
      • Mouse
      Antibody TypePolyclonal Antibody
      Purification MethodImmunoAffinity Purified
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsThe lyophilized antibody-conjugate is stable at +4°C.The reconstituted antibody conjugate solution is stable for 3 months at +4°C.The solution should be stored in aliquots at -20°C; repeated freezing and thawing should be avoided.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information



      Reference overviewPub Med ID
      De novo generation of satellite DNA-based artificial chromosomes by induced large-scale amplification.
      Erika Csonka
      Methods in molecular biology (Clifton, N.J.) 738 2011

      Show Abstract
      21431723 21431723
      Monoclonal antibodies to a nuclear protein (PCNA/cyclin) associated with DNA replication.
      Ogata, K, et al.
      Exp. Cell Res., 168: 475-86 (1987) 1987

      Show Abstract
      2879737 2879737
      Detection of plasma cell immunoglobulins in tissue sections optimally fixed for ultrastructural immunocytochemistry.
      Gonatas, N K, et al.
      J. Histochem. Cytochem., 35: 189-96 (1987) 1987

      Show Abstract
      3098833 3098833
      A cell ELISA for the quantitation of leukocyte antigens. Requirements for calibration.
      Baumgarten, H
      J. Immunol. Methods, 94: 91-8 (1986) 1986

      3782820 3782820
      Utilization of the biotin/avidin system to amplify the sensitivity of the enzyme-linked immunosorbent assay (ELISA).
      Kendall, C, et al.
      J. Immunol. Methods, 56: 329-39 (1983) 1983

      Show Abstract
      6833765 6833765

      Data Sheet

      Sheep anti-Mouse Ig, (H+L) Digoxigenin Conjugated Affinity Purified F(ab')2 antibody - Data Sheet

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