17-127 | Ser/Thr Phosphatase Assay Kit 1 (K-R-pT-I-R-R)

17-127
1 kit  Kit capacity: 100 assays
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      Overview

      Replacement Information

      Key Specifications Table

      Key ApplicationsAnalytes AvailableDetection Methods
      PA Ser/Thr Kinases Colorimetric
      Description
      Catalogue Number17-127
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionSer/Thr Phosphatase Assay Kit 1 (K-R-pT-I-R-R)
      References
      Product Information
      Components
      • Malachite Green Solution A (Cat.# 20-105)
      • Malachite Green Additive (Cat.# 20-104)
      • Phosphate Standard (Cat.# 20-103)
      • Threonine Phosphopeptide (K-R-pT-I-R-R) (Cat.# 12-219)
      • pNPP (p-Nitrophenyl Phosphate) (Cat.# 20-106)
      • pNPP Ser/Thr Assay Buffer (Cat.# 20-179)
      • NiCl2, 40mM (Cat.# 20-178)
      • 96-well microtiter plate
      Detection methodColorimetric
      Applications
      ApplicationSer/Thr Phosphatase Assay Kit 1used to detect PP2A activity by either dephosphorylation of the phosphopeptide.
      Key Applications
      • Phosphatase Assay
      Biological Information
      Analytes Available
      • Ser/Thr Kinases
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes a beta isoform of the constant regulatory subunit A. Defects in this gene could be the cause of some lung and colon cancers. At least two transcript variants encoding different isoforms have been found for this gene.
      Gene Symbol
      • PPP2R1B
      • PP2A-A-beta
      • MGC26454
      • PR65B
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P30154 # The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit.
      SIZE: 601 amino acids; 66214 Da
      SUBUNIT: PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with IPO9.
      DOMAIN: SwissProt: P30154 Each HEAT repeat appears to consist of two alpha helices joined by a hydrophilic region, the intrarepeat loop. The repeat units may be arranged laterally to form a rod-like structure.
      DISEASE:SwissProt: P30154 # Defects in PPP2R1B might be a cause of some lung and colorectal cancers.
      SIMILARITY: Belongs to the phosphatase 2A regulatory subunit A family. & Contains 15 HEAT repeats.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely used to detect PP2A (Catalog # 14-111) activity by either dephosphorylation of the phosphopeptide (K-R-pT-I-R-R) or hydrolysis of pNPP
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size1 kit
      Material PackageKit capacity: 100 assays
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Ser/Thr Phosphatase Assay Kit 1 2470978
      Ser/Thr Phosphatase Assay Kit 1 (K-R-pT-I-R-R) - 2135689 2135689
      Ser/Thr Phosphatase Assay Kit 1 (K-R-pT-I-R-R) - 2391133 2391133
      Ser/Thr Phosphatase Assay Kit 1 (K-R-pT-I-R-R) 2884453
      Ser/Thr Phosphatase Assay Kit 1 (K-R-pT-I-R-R) 2899545
      Ser/Thr Phosphatase Assay Kit 1 (K-R-pT-I-R-R) - 16790 16790
      Ser/Thr Phosphatase Assay Kit 1 (K-R-pT-I-R-R) - 17648 17648
      Ser/Thr Phosphatase Assay Kit 1 (K-R-pT-I-R-R) - 18338 18338
      Ser/Thr Phosphatase Assay Kit 1 (K-R-pT-I-R-R) - 19354 19354
      Ser/Thr Phosphatase Assay Kit 1 (K-R-pT-I-R-R) - 20609 20609

      References

      Reference overviewApplicationPub Med ID
      Antiproliferative mechanisms of action of the flavin dehydrogenase inhibitors diphenylene iodonium and di-2-thienyliodonium based on molecular profiling of the NCI-60 human tumor cell panel.
      James H Doroshow,Agnes Juhasz,Yun Ge,Susan Holbeck,Jiamo Lu,Smitha Antony,Yongzhong Wu,Guojian Jiang,Krishnendu Roy
      Biochemical pharmacology  83  2012

      Show Abstract
      22305747 22305747
      Transmigration of beta amyloid specific heavy chain antibody fragments across the in vitro blood-brain barrier.
      Rutgers KS, Nabuurs RJ, van den Berg SA, Schenk GJ, Rotman M, Verrips CT, van Duinen SG, Maat-Schieman ML, van Buchem MA, de Boer AG, van der Maarel SM
      Neuroscience  2011

      Show Abstract
      21683126 21683126
      Failure of stress-induced downregulation of Bcl-2 contributes to apoptosis resistance in senescent human diploid fibroblasts.
      S J Ryu, Y S Oh, S C Park
      Cell death and differentiation  14  1020-8  2007

      Show Abstract
      17290286 17290286
      FMRP phosphorylation reveals an immediate-early signaling pathway triggered by group I mGluR and mediated by PP2A.
      Usha Narayanan, Vijayalaxmi Nalavadi, Mika Nakamoto, David C Pallas, Stephanie Ceman, Gary J Bassell, Stephen T Warren
      The Journal of neuroscience : the official journal of the Society for Neuroscience  27  14349-57  2007

      Show Abstract
      18160642 18160642
      Specific beta1 integrin site selectively regulates Akt/protein kinase B signaling via local activation of protein phosphatase 2A.
      Pankov, Roumen, et al.
      J. Biol. Chem., 278: 18671-81 (2003)  2003

      Show Abstract
      Phosphatase Assay12637511 12637511
      Reduced expression of the regulatory A subunit of serine/threonine protein phosphatase 2A in human breast cancer MCF-7 cells
      Suzuki, K. and Takahashi, K.
      Int J Oncol, 23:1263-8 (2003)  2003

      Phosphatase Assay14532964 14532964
      N-Ethylmaleimide inhibits platelet-derived growth factor BB-stimulated Akt phosphorylation via activation of protein phosphatase 2A.
      Yellaturu, CR; Bhanoori, M; Neeli, I; Rao, GN
      The Journal of biological chemistry  277  40148-55  2002

      Show Abstract
      12171932 12171932
      Serine (threonine) phosphatase(s) acting on cAMP-dependent phosphoproteins in mammalian mitochondria.
      Signorile A, Sardanelli AM, Nuzzi R, Papa S.
      FEBS letters  512  91-4  2001

      Show Abstract
      11852058 11852058
      The serine phosphatases PP1 and PP2A associate with and activate the actin-binding protein cofilin in human T lymphocytes
      Ambach, A., et al
      Eur J Immunol, 30:3422-31 (2000)  2000

      Phosphatase Assay11093160 11093160
      Regulation of protein phosphatase 2A activity by caspase-3 during apoptosis
      Santoro, M. F., et al
      J Biol Chem, 273:13119-28 (1998)  1998

      Phosphatase Assay9582351 9582351

      Related Products & Applications

      Kit Component

      Catalog Number Description  
      12-219 Threonine Phosphopeptide (K-R-pT-I-R-R) Show Pricing & Availability
      20-103 Phosphate Standard Show Pricing & Availability
      20-104 Malachite Green Additive Show Pricing & Availability
      20-105 Malachite Green Solution A Show Pricing & Availability
      20-106 pNPP (p-Nitrophenyl Phosphate) Show Pricing & Availability
      20-179 pNPP Ser/Thr Assay Buffer Show Pricing & Availability

      Categories

      Life Science Research > Protein Detection and Quantification > Activity Assays > Phosphatase Assays