Pricing & Availability
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|71136-3||Plastic ampoule||0.4 ml||
|71136-4||Glass bottle||1 ml||
|Overview||Rosetta-gami B strains combine the key features of BL21 (and its Tuner™ derivative), Origami, and Rosetta to enhance both the expression of eukaryotic proteins and the formation of target protein disulfide bonds in the bacterial cytoplasm. These strains are compatible with ampicillin- or spectinomycin-resistant vectors.
DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
|Genotype: F– ompT hsdSB (rB– mB–) gal dcm lacY1 ahpC (DE3) gor522::Tn10 trxB pRARE (CamR, KanR, TetR)
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
|0.4 ml||1 ml||Component|
|•||2 × 0.2 ml||5 × 0.2 ml||Rosetta-gami B(DE3) Competent Cells|
|•||2 × 2 ml||4 × 2 ml||SOC Medium|
|•||10 µl||10 µl||Test Plasmid|
|Safety Information according to GHS|
|Product Usage Statements|
|Storage and Shipping Information|
|Ship Code||Dry Ice Only|
|Toxicity||Multiple Toxicity Values, refer to MSDS|
|Avoid freeze/thaw||Avoid freeze/thaw|
|Do not freeze||Ok to freeze|
Rosetta-gami B(DE3) Competent Cells - Novagen SDS
|Competent Cell Brochure|