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71136 | Rosetta-gami B(DE3) Competent Cells - Novagen

71136
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      Overview

      Replacement Information

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      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      71136-3
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          Glass bottle 0.4 ml
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          71136-4
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              Glass bottle 1 ml
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              Description
              OverviewRosetta-gami B strains combine the key features of BL21 (and its Tuner™ derivative), Origami, and Rosetta to enhance both the expression of eukaryotic proteins and the formation of target protein disulfide bonds in the bacterial cytoplasm. These strains are compatible with ampicillin- or spectinomycin-resistant vectors.

              DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
              Genotype: F ompT hsdSB (rB mB) gal dcm lacY1 ahpC (DE3) gor522::Tn10 trxB pRARE (CamR, KanR, TetR)
              Catalogue Number71136
              Brand Family Novagen®
              References
              Product Information
              0.4 ml1 mlComponent
              2 × 0.2 ml5 × 0.2 mlRosetta-gami B(DE3) Competent Cells
              2 × 2 ml4 × 2 mlSOC Medium
              10 µl10 µlTest Plasmid
              Applications
              Biological Information
              Physicochemical Information
              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              Safety Information
              Product Usage Statements
              Storage and Shipping Information
              Ship Code Dry Ice Only
              Toxicity Multiple Toxicity Values, refer to MSDS
              Storage ≤ -70°C
              Avoid freeze/thaw Avoid freeze/thaw
              Do not freeze Ok to freeze
              Packaging Information
              Transport Information
              Supplemental Information
              Specifications

              Documentation

              SDS

              Title

              Safety Data Sheet (SDS) 

              Certificates of Analysis

              TitleLot Number
              71136

              Brochure

              Title
              Competent Cell Brochure

              Citations

              Title
            • Wei Cao, et al. (2007) RAET1E2, a soluble isoform of the UL16 binding protein RAET1E produced by tumor cells, inhibits NKG2D-mediated NK cytotoxicity.. Journal of Biological Chemistry 282, 18922-18928.
            • Christian Zuber, et al. (2007) EDEM1 reveals a quality control vesicular transport pathway out of the endoplasmic reticulum not involving the COPII exit sites. Procedings of the National Academy of Science 104, 4407-4412.
            • Jinghua Chen, et al. (2005) Enzymatic redesigning of biologically active heparan sulfate. Journal of Biological Chemistry 280, 42817-42825.
            • Karsten Mahnke, et al. (2005) Targeting of antigens to activated dendritic cells in vivo cures metastatic melanoma in mice. Cancer Research 65, 7007-7012.
            • John Sergeant, et al. (2005) Composition and architecture of the Schizosaccharomyces pombe rad18 (Smc5-6) complex. Molecular and Cellular Biology 25, 172-184.
            • Atsushi Takabayashi, et al. (2005) Differential use of two cyclic electron flows around photosystem I for driving CO2-concentration mechanism in C4 photosynthesis. Proceedings of the National Academy of Sciences (USA) 102, 16898-16903.
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              Categories

              Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Competent Cells for Expression