71400 Rosetta™ 2(DE3) Singles™ Competent Cells - Novagen

71400
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          Plastic ampoule 11 rxn
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              Glass bottle 22 rxn
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              Description
              OverviewA common method for transformation of DNA plasmids into E. coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen prepared competent cells. Novagen® Singles™ Competent Cells are designed for ultimate convenience and reliability in plasmid transformation. Chemically competent cells are prepared using an optimized method. Cells are provided in 50µl volumes, eliminating the need to aliquot, freeze/thaw, or waste partially used vials, saving time and money and ensuring reliable cell performance. To use, simply thaw, add DNA, incubate 5 minutes on ice, heat shock for 30 seconds, place on ice for 2 minutes, and add SOC medium. T7 expression strains are lysogens of bacteriophage DE3, as indicated by the (DE3). These hosts carry a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in appropriate T7 expression vectors, using IPTG as an inducer.
              Rosetta™ 2 host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. These strains supply tRNAs for 7 rare codones (AGA, AGG, AUA, CUA, GGA, CCC, and CGG) on a compatible chloramphenicol-resistant plasmid. The tRNA genes are driven by their native promoters.

              DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
              Genotype: F- ompT hsdSB(rB- mB-) gal dcm (DE3) pRARE2 (CamR)


              This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
              Catalogue Number71400
              Brand Family Novagen®
              References
              Product Information
              11 rxn or 22 rxnRosetta 2(DE3) Competent Cells
              2 × 2 ml or 4 × 2 mlSOC Medium
              10 µlTest Plasmid
              Guaranteed efficiency>2 × 10⁶ cfu/µg
              Applications
              Biological Information
              Physicochemical Information
              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              Safety Information
              Product Usage Statements
              Storage and Shipping Information
              Ship Code Dry Ice Only
              Toxicity Multiple Toxicity Values, refer to MSDS
              Storage ≤ -70°C
              Do not freeze Ok to freeze
              Packaging Information
              Transport Information
              Supplemental Information
              Specifications

              Documentation

              Rosetta™ 2(DE3) Singles™ Competent Cells - Novagen SDS

              Title

              Safety Data Sheet (SDS) 

              Rosetta™ 2(DE3) Singles™ Competent Cells - Novagen Certificates of Analysis

              TitleLot Number
              71400

              Brochure

              Title
              The Complete Molecular Biology Toolkit - Expert workflow solutions from DNA cloning to protein expression (EMD)

              Citations

              Title
            • Taj Kattapuram, et al. (2005) Protein kinase CK1α regulates mRNA binding by hnRNP-C in response to physiologic levels of hydrogen peroxide. Journal of Biological Chemistry 280, 15340-15347.
            • User Protocols

              Title
              TB055 pET System Manual

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              Categories

              Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Competent Cells for Expression