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14-383 | Rho Assay Reagent (Rhotekin RBD, agarose), 650 µg

14-383
650 µg  
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      Overview

      Replacement Information
      Description
      Catalogue Number14-383
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionRho Assay Reagent (Rhotekin RBD, agarose), 650 µg
      OverviewGST-tagged fusion protein, corresponding to residues 7-89 of mouse Rhotekin Rho Binding Domain, expressed in E.coli and bound to glutathione-agarose. Specifically binds to and precipitates GTP-Rho, but not GDP-Rho from cell lysates.
      References
      Product Information
      Presentation650 µg of recombinant protein in 1 mL glutathione-agarose slurry containing 45mM TRIS, pH 7.5, 0.45% Triton X-100, 135mM NaCl, 4.5mM MgCl2, 0.9mM dithiothreitol, 0.09mM PMSF, 0.9 µg/mL aprotinin, 0.9 µg/mL leupeptin, and 10% glycerol.
      Applications
      ApplicationSpecifically binds to & precipitates GTP-Rho, but not GDP-Rho from cell lysates. For use in Affinity Binding Assays.
      Key Applications
      • Affinity Binding Assay
      Biological Information
      SourceExpressed in E. coli
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a scaffold protein that interacts with GTP-bound Rho proteins. Binding of this protein inhibits the GTPase activity of Rho proteins. This protein may interfere with the conversion of active, GTP-bound Rho to the inactive GDP-bound form by RhoGAP. Rho proteins regulate many important cellular processes, including cytokinesis, transcription, smooth muscle contraction, cell growth and transformation. Dysregulation of the Rho signal transduction pathway has been implicated in many forms of cancer. Alternative splicing results in multiple transcript variants encoding different isoforms.
      Gene Symbol
      • RTKN
      • Rhotekin.
      • rhotekin
      • B5
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q9BST9 # Mediates Rho signaling to activate NF-kappa-B and may confer increased resistance to apoptosis to cells in gastric tumorigenesis. May play a novel role in the organization of septin structures.
      SIZE: 563 amino acids; 62667 Da
      SUBUNIT: Interacts via its C-terminal region with the TAX1BP3 PDZ domain. This interaction facilitates Rho-mediated activation of the c-Fos serum response element (SRE). Interacts with SEPT9. Specifically binds to GTP-bound RHOA, RHOB and RHOC and inhibits their GTPase activity.
      TISSUE SPECIFICITY: Highly expressed in prostate, moderately in kidney, heart, brain, spleen, testis, placenta, small intestine, pancreas, skeletal muscle and peripheral blood leukocytes, and weakly in ovary, colon and thymus. Weakly expressed in all normal cell lines tested. Overexpressed in various cancer cell lines.
      SIMILARITY: SwissProt: Q9BST9 ## Contains 1 PH domain. & Contains 1 REM (Hr1) repeat.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by precipitating GTP-Rho from 3T3/A31 lysates, subsequent detection by immunoblot analysis using Anti-RhoA, clone 55 (05-778).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 6 months at -70°C from date of receipt.
      Packaging Information
      Material Size650 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Rho Assay Reagent (Rhotekin RBD, agarose) 2388940
      Rho Assay Reagent (Rhotekin RBD, agarose) (recombinant protein expressed in E. coli) - 2117740 2117740
      Rho Assay Reagent (Rhotekin RBD, agarose) (recombinant protein expressed in E. coli) - 2343892 2343892
      Rho Assay Reagent (Rhotekin RBD, agarose) (recombinant protein expressed in E. coli) - 2013105 2013105
      Rho Assay Reagent (Rhotekin RBD, agarose) (recombinant protein expressed in E. coli) - 2167792 2167792
      Rho Assay Reagent (Rhotekin RBD, agarose) (recombinant protein expressed in E. coli) - 2200936 2200936
      Rho Assay Reagent (Rhotekin RBD, agarose) (recombinant protein expressed in E. coli) - 2250496 2250496
      Rho Assay Reagent (Rhotekin RBD, agarose) (recombinant protein expressed in E. coli) -2502989 2502989
      Rho Assay Reagent (Rhotekin RBD, agarose) - 20171 20171
      Rho Assay Reagent (Rhotekin RBD, agarose) - 2064528 2064528
      Rho Assay Reagent (Rhotekin RBD, agarose) - 21145 21145
      Rho Assay Reagent (Rhotekin RBD, agarose) - 21341 21341
      Rho Assay Reagent (Rhotekin RBD, agarose) - 21528 21528
      Rho Assay Reagent (Rhotekin RBD, agarose) - 21769 21769
      Rho Assay Reagent (Rhotekin RBD, agarose) - 22903 22903
      Rho Assay Reagent (Rhotekin RBD, agarose) - 23673 23673
      Rho Assay Reagent (Rhotekin RBD, agarose) - 25293 25293
      Rho Assay Reagent (Rhotekin RBD, agarose) - 25924 25924
      Rho Assay Reagent (Rhotekin RBD, agarose) - 27344 27344
      Rho Assay Reagent (Rhotekin RBD, agarose) - 28751 28751
      Rho Assay Reagent (Rhotekin RBD, agarose) - 31557 31557
      Rho Assay Reagent (Rhotekin RBD, agarose) - D6C029 D6C029
      Rho Assay Reagent (Rhotekin RBD, agarose) - DAM1514737 DAM1514737
      Rho Assay Reagent (Rhotekin RBD, agarose) - DAM1570866 DAM1570866
      Rho Assay Reagent (Rhotekin RBD, agarose) - DAM1724579 DAM1724579
      Rho Assay Reagent (Rhotekin RBD, agarose) - DAM1791770 DAM1791770
      Rho Assay Reagent (Rhotekin RBD, agarose) - R0610D0002 R0610D0002
      Rho Assay Reagent (Rhotekin RBD, agarose) - R0703D0002 R0703D0002
      Rho Assay Reagent (Rhotekin RBD, agarose) - R0712D0002 R0712D0002
      Rho Assay Reagent (Rhotekin RBD, agarose) -2606904 2606904
      Rho Assay Reagent (Rhotekin RBD, agarose) -2618840 2618840
      Rho Assay Reagent (Rhotekin RBD, agarose) -2685484 2685484
      Rho Assay Reagent (Rhotekin RBD, agarose) -2691235 2691235
      Rho Assay Reagent (Rhotekin RBD, agarose) -2717642 2717642
      Rho Assay Reagent (Rhotekin RBD, agarose) -2780502 2780502

      References

      Reference overviewApplicationPub Med ID
      Geranylgeranyl diphosphate depletion inhibits breast cancer cell migration.
      Amel Dudakovic,Huaxiang Tong,Raymond J Hohl
      Investigational new drugs 29 2011

      Show Abstract
      20480384 20480384
      Vascular endothelial growth factor C promotes cervical cancer metastasis via up-regulation and activation of RhoA/ROCK-2/moesin cascade.
      He, M; Cheng, Y; Li, W; Liu, Q; Liu, J; Huang, J; Fu, X
      BMC cancer 10 170 2010

      Show Abstract Full Text Article
      Western Blotting20429915 20429915
      Functional SNP of ARHGEF10 confers risk of atherothrombotic stroke.
      Matsushita T, Ashikawa K, Yonemoto K, Hirakawa Y, Hata J, Amitani H, Doi Y, Ninomiya T, Kitazono T, Ibayashi S, Iida M, Nakamura Y, Kiyohara Y, Kubo M
      Human molecular genetics 19 1137-46 2010

      Show Abstract
      20042462 20042462
      Extracellular matrix controls insulin signaling in mammary epithelial cells through the RhoA/Rok pathway.
      Lee, Yi-Ju, et al.
      J. Cell. Physiol., 220: 476-84 (2009) 2009

      Show Abstract
      19391109 19391109
      Progestogens regulate endothelial actin cytoskeleton and cell movement via the actin-binding protein moesin.
      Xiao-Dong Fu, Marina Flamini, Angel Matias Sanchez, Lorenzo Goglia, Maria Silvia Giretti, Andrea R Genazzani, Tommaso Simoncini
      Molecular human reproduction 14 225-34 2008

      Show Abstract
      18308736 18308736
      Simvastatin reduces human atrial myofibroblast proliferation independently of cholesterol lowering via inhibition of RhoA
      Porter, K. E., et al
      Cardiovasc Res, 61:745-55 (2004) 2004

      Activation Assay14985071 14985071
      Cooperativity between the Ras-ERK and Rho-Rho kinase pathways in urokinase-type plasminogen activator-stimulated cell migration
      Jo, M., et al
      J Biol Chem, 277:12479-85 (2002) 2002

      Activation Assay11805108 11805108
      Determination of GTP loading on Rho.
      Ren, X D and Schwartz, M A
      Meth. Enzymol., 325: 264-72 (2000) 2000

      11036609 11036609
      Regulation of the small GTP-binding protein Rho by cell adhesion and the cytoskeleton
      Ren, X D, et al
      EMBO J, 18:578-85 (1999) 1999

      9927417 9927417
      Rhotekin, a new putative target for Rho bearing homology to a serine/threonine kinase, PKN, and rhophilin in the rho-binding domain.
      Reid, T, et al.
      J. Biol. Chem., 271: 13556-60 (1996) 1996

      Show Abstract
      Activation Assay8662891 8662891