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17-294 | Rho Activation Assay Kit

1 kit  Kit capacity: 30 assays
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      Replacement Information

      Key Specifications Table

      Key ApplicationsDetection Methods
      ABA Fluorescent, Chemiluminescence, Colorimetric
      Catalogue Number17-294
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionRho Activation Assay Kit
      OverviewA GST-tagged fusion protein, corresponding to residues 7-89 of mouse Rhotekin Rho Binding Domain, expressed in E. coli. Provided bound to glutathione-agarose. Purity >90%.
      Product Information
      • 100X GTPγS, 10mM (Cat.# 20-176)
      • 100X GDP, 100mM (Cat.# 20-177)
      • Mg2+ Lysis/Wash Buffer, 5X (Cat.# 20-168)
      • Anti-Rho (-A, -B, -C), clone 55 (Cat.# 05-778)
      • Rho Assay Reagent (Rhotekin RBD, agarose) (Cat.# 14-383)
      Detection methodFluorescent Chemiluminescence Colorimetric
      ApplicationPP1/PP2A Toolbox for the selective in vitro dephosphorylation of proteins.
      Key Applications
      • Affinity Binding Assay
      Biological Information
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The protein encoded by this gene is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane. It is thought to be important in cell locomotion. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.
      Gene Symbol
      • RHOA
      • RHO12
      • Rho12
      • RhoA
      • ARHA
      • ARH12
      • RHOH12
      • H12
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P62745 # Mediates apoptosis in neoplastically transformed cells after DNA damage. Not essential for development but affects cell adhesion and growth factor signaling in transformed cells. Plays a negative role in tumorigenesis as deletion causes tumor formation. Involved in intracellular protein trafficking of a number of proteins. Targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes. Also required for stability and nuclear trafficking of AKT1/AKT which promotes endothelial cell survival during vascular development.
      SIZE: 196 amino acids; 22123 Da
      SUBUNIT: Binds ROCK1 and ROCK2. Also binds PKN1/PRK1. Interacts with ARGGEF3, RTKN and AKAP13.
      SUBCELLULAR LOCATION: Late endosome membrane; Lipid-anchor. Cell membrane; Lipid-anchor. Nucleus. Note=Late endosomal membrane (geranylgeranylated form). Plasma membrane (farnesylated form). Also detected at the nuclear margin and in the nucleus.
      PTM: Prenylation specifies the subcellular location of RHOB. The farnesylated form is localized to the plasma membrane while the geranylgeranylated form is localized to the endosome.
      SIMILARITY: SwissProt: P62745 ## Belongs to the small GTPase superfamily. Rho family.
      MISCELLANEOUS: RHOB is one of the targets of farnesyltransferase inhibitors which are currently under investigation as cancer therapeutics. These elevate the levels of geranylgeranylated RHOB and cause mislocalization, leading to apoptosis and antineoplastic effects.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by precipitating GTP- Rho from 3T3/A31 cell lysates, subsequent detection by immunoblot analysis using anti-Rho (05-778).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size1 kit
      Material PackageKit capacity: 30 assays
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Rho Activation Assay Kit 2470979
      Rho Activation Assay Kit - 2390497 2390497
      Rho Activation Assay Kit - 0607034578 0607034578
      Rho Activation Assay Kit - 0611044536 0611044536
      Rho Activation Assay Kit - 0611046445 0611046445
      Rho Activation Assay Kit - 0701049344 0701049344
      Rho Activation Assay Kit - 1946442 1946442
      Rho Activation Assay Kit - 1969890 1969890
      Rho Activation Assay Kit - 20196 20196
      Rho Activation Assay Kit - 2026219 2026219
      Rho Activation Assay Kit - 2043486 2043486
      Rho Activation Assay Kit - 2070557 2070557
      Rho Activation Assay Kit - 2074902 2074902
      Rho Activation Assay Kit - 21144 21144
      Rho Activation Assay Kit - 21308 21308
      Rho Activation Assay Kit - 21431 21431
      Rho Activation Assay Kit - 2151070 2151070
      Rho Activation Assay Kit - 21697 21697
      Rho Activation Assay Kit - 21929 21929
      Rho Activation Assay Kit - 2200938 2200938
      Rho Activation Assay Kit - 2225262 2225262
      Rho Activation Assay Kit - 2276355 2276355
      Rho Activation Assay Kit - 23049 23049
      Rho Activation Assay Kit - 2330570 2330570
      Rho Activation Assay Kit - 23415 23415
      Rho Activation Assay Kit - 23875 23875
      Rho Activation Assay Kit - 2491192 2491192
      Rho Activation Assay Kit - 27778 27778
      Rho Activation Assay Kit - 28785 28785
      Rho Activation Assay Kit - 29606 29606
      Rho Activation Assay Kit - 29884 29884
      Rho Activation Assay Kit - 29884A 29884A
      Rho Activation Assay Kit - 30284 30284
      Rho Activation Assay Kit - 31508 31508
      Rho Activation Assay Kit - 32021 32021
      Rho Activation Assay Kit - 33502 33502
      Rho Activation Assay Kit - 33633 33633
      Rho Activation Assay Kit - DAM1487504 DAM1487504
      Rho Activation Assay Kit - DAM1574301A DAM1574301A
      Rho Activation Assay Kit - DAM1635758 DAM1635758
      Rho Activation Assay Kit - DAM1650043 DAM1650043
      Rho Activation Assay Kit - DAM1764537 DAM1764537
      Rho Activation Assay Kit - JBC1351424 JBC1351424
      Rho Activation Assay Kit - JBC1371827 JBC1371827
      Rho Activation Assay Kit - JBC1382006A JBC1382006A
      Rho Activation Assay Kit - JBC1393237A JBC1393237A
      Rho Activation Assay Kit - JBC1884500 JBC1884500
      Rho Activation Assay Kit - JBC1903653 JBC1903653
      Rho Activation Assay Kit - JBC1924375 JBC1924375
      Rho Activation Assay Kit -2618654 2618654
      Rho Activation Assay Kit -2686958 2686958
      Rho Activation Assay Kit -2707876 2707876
      Rho Activation Assay Kit -2726822 2726822
      Rho Activation Assay Kit -2772674 2772674

      References | 19 Available | See All References

      Reference overviewApplicationPub Med ID
      Prostaglandin E2 Inhibits Human Lung Fibroblast Chemotaxis through Disparate Actions on Different E-Prostanoid Receptors.
      Li YJ, Wang XQ, Sato T, Kanaji N, Nakanishi M, Kim M, Michalski J, Nelson AJ, Sun JH, Farid M, Basma H, Patil A, Toews ML, Liu X, Rennard SI
      Am J Respir Cell Mol Biol 2010

      Show Abstract
      20203295 20203295
      Cyclin-dependent kinase 5 regulates endothelial cell migration and angiogenesis.
      Johanna Liebl,Sabine B Weitensteiner,György Vereb,Lili Takács,Robert Fürst,Angelika M Vollmar,Stefan Zahler
      The Journal of biological chemistry 285 2010

      Show Abstract Full Text Article
      20826806 20826806
      Attenuation of angiotensin II-induced hypertension and cardiac hypertrophy in transgenic mice overexpressing a type 1 receptor mutant.
      Saad Ahmad,Francesca Cesana,Edward Lamperti,Haralambos Gavras,Jun Yu
      American journal of hypertension 22 2009

      Show Abstract Full Text Article
      19779471 19779471
      Pulmonary microvascular endothelial cells form a tighter monolayer when grown in chronic hypoxia.
      Victor Solodushko, James C Parker, Brian Fouty
      American journal of respiratory cell and molecular biology 38 491-7 2008

      Show Abstract Full Text Article
      18048805 18048805
      Sphingosine 1-phosphate potentiates human lung fibroblast chemotaxis through the S1P2 receptor.
      Mitsu Hashimoto, Xingqi Wang, Lijun Mao, Tetsu Kobayashi, Shin Kawasaki, Naoyoshi Mori, Myron L Toews, Hui Jung Kim, D Roselyn Cerutis, Xiangde Liu, Stephen I Rennard
      American journal of respiratory cell and molecular biology 39 356-63 2008

      Show Abstract Full Text Article
      18367729 18367729
      Atorvastatin decreases lipoprotein lipase and endothelial lipase expression in human THP-1 macrophages.
      Guosong Qiu, John S Hill
      Journal of lipid research 48 2112-22 2007

      Show Abstract
      17644777 17644777
      Rho signalling at a glance.
      Schwartz, Martin
      J. Cell. Sci., 117: 5457-8 (2004) 2004

      15509861 15509861
      Protein kinase A-mediated phosphorylation of the Galpha13 switch I region alters the Galphabetagamma13-G protein-coupled receptor complex and inhibits Rho activation
      Manganello, J. M., et al
      J Biol Chem, 278:124-30 (2003) 2003

      Activation Assay12399457 12399457
      PTEN regulates RANKL- and osteopontin-stimulated signal transduction during osteoclast differentiation and cell motility
      Sugatani, T., et al
      J Biol Chem, 278:5001-8 (2003) 2003

      Activation Assay12460992 12460992
      Protein kinase involved in lung injury susceptibility: Evidence from enzyme isoform genetic knockout and in vivo inhibitor treatment
      Wainwright, M. S., et al
      Proc Natl Acad Sci U S A, 100:6233-8 (2003) 2003

      Activation Assay12730364 12730364


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      Related Products & Applications

      Kit Component

      Catalog Number Description  
      05-778 Anti-Rho (-A Antibody, -B, -C), clone 55 Show Pricing & Availability
      14-383 Rho Assay Reagent (Rhotekin RBD, agarose), 650 µg Show Pricing & Availability
      20-168 Mg2+ Lysis/Wash Buffer, 5X Show Pricing & Availability
      20-176 100X GTPγS, 10mM Show Pricing & Availability
      20-177 100X GDP, 100mM Show Pricing & Availability


      Life Science Research > Protein Detection and Quantification > Activity Assays > Activation Assays