554715 Retro-2 - Calbiochem

554715
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      Overview

      Replacement Information

      Key Specifications Table

      Empirical Formula
      C₁₉H₁₆N₂OS

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      554715-25MG
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          Glass bottle 25 mg
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          Description
          OverviewA cell-permeable tricyclic imine compound that inhibits the endosome-to-Golgi retrograde transport, but not cellular uptake, of CtxB (Cholera toxin B chain), StxB (Shiga-like toxin B chain), and the plant toxin ricin from castor seed in HeLa cells (20 µM), without affecting cellular uptake and trafficking of Tf (Transferrin), EGF, and vesicular stomatitis virus glycoprotein VSVGts045. Retro-2 is demonstrated to selectively affect the cellular distribution of SNARE proteins Synt5 and Synt6, but not 24 other Golgi and endocytic pathway proteins. Retro-2 pretreatment is shown to effectively protect against ricin-, Stx1-, and Stx2-induced cytotoxicity in HeLa cultures in vitro (Fold of protection against 4 h toxin incubation = 2.7, 22, and 65, respectively, with 0.5 h 20 µM Retro-2 pretreatment), as well as ricin-induced (2 µg/kg by nasal spray) fatality in mice in vivo (0 mortality with one 200 mg/kg i.p. dose 1h prior to toxin exposure).
          Catalogue Number554715
          Brand Family Calbiochem®
          SynonymsCholera Toxin Retrograde Transport Blocker, Ricin Retrograde Transport Blocker, SLT Retrograde Transport Blocker, 2-(((5-Methyl-2-thienyl)methylene)amino)-N-phenylbenzamide
          References
          ReferencesStechmann, B., et al. 2010. Cell 141, 231.
          Product Information
          FormTan powder
          Hill FormulaC₁₉H₁₆N₂OS
          Chemical formulaC₁₉H₁₆N₂OS
          Structure formula ImageStructure formula Image
          Applications
          Biological Information
          Purity≥95% by HPLC
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Protect from Light Protect from light
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
          Packaging Information
          Packaged under inert gas Packaged under inert gas
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Retro-2 - Calbiochem SDS

          Title

          Safety Data Sheet (SDS) 

          Retro-2 - Calbiochem Certificates of Analysis

          TitleLot Number
          554715

          References

          Reference overview
          Stechmann, B., et al. 2010. Cell 141, 231.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision24-October-2011 RFH
          SynonymsCholera Toxin Retrograde Transport Blocker, Ricin Retrograde Transport Blocker, SLT Retrograde Transport Blocker, 2-(((5-Methyl-2-thienyl)methylene)amino)-N-phenylbenzamide
          DescriptionA cell-permeable tricyclic imine compound that inhibits the endosome-to-Golgi retrograde transport, but not cellular uptake, of CtxB (Cholera toxin B chain), StxB (Shiga-like toxin B chain), and the plant toxin ricin from castor seed in HeLa cells (20 µM), without affecting cellular uptake and trafficking of Tf (Transferrin), EGF, and vesicular stomatitis virus glycoprotein VSVGts045. Retro-2 is demonstrated to selectively affect the cellular distribution of SNARE proteins Synt5 and Synt6, but not 24 other Golgi and endocytic pathway proteins. Retro-2 pretreatment is shown to effectively protect against ricin-, Stx1-, and Stx2-induced cytotoxicity in HeLa cultures in vitro (Fold of protection against 4 h toxin incubation = 2.7, 22, and 65, respectively, with 0.5 h 20 µM Retro-2 pretreatment), as well as ricin-induced (2 µg/kg by nasal spray) fatality in mice in vivo (0 mortality with one 200 mg/kg i.p. dose 1h prior to toxin exposure).
          FormTan powder
          Intert gas (Yes/No) Packaged under inert gas
          Chemical formulaC₁₉H₁₆N₂OS
          Structure formulaStructure formula
          Purity≥95% by HPLC
          SolubilityDMSO (50 mg/ml). It is recommended to prepare a stock solution for 3 month use each time and store the unreconstituted material in solid form, protected from moisture and preferably under inert gas, for best long-term stability during storage.
          Storage +2°C to +8°C
          Protect from light
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
          Toxicity Standard Handling
          ReferencesStechmann, B., et al. 2010. Cell 141, 231.