530353 Regacin - CAS 72836-33-0 - Calbiochem

530353
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      Overview

      Replacement Information

      Key Specifications Table

      Empirical FormulaCAS #
      C₉H₈ClN₃S₂ 72836-33-0

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      5.30353.0001
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          Glass bottle 25 mg
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          Description
          OverviewAn orally bioavailable, non-toxic, thio-thiadiazolamine compound that specifically inhibits the DNA-binding capacity of virulence regulator, RegA (IC50 = 1.7 µM in E. coli strain MC4100 (kfc-lacZ, pACYC184-regA), by interacting with amino acid residues within a conserved region of the DNA-binding domain, but without affecting RegA dimerization. Interacts with the double helix-turn-helix (HTH) domain that results in a loss of binding affinity of RegA for its DNA targets. This interaction appears to involve W188 and R223 residues that are conserved in several RegA homologs including ToxT, AggR, RegR, and Rns. Shown to be highly effective in reducing Citrobacter rodentium colonization when administered perorally to mice (~50 mg/kg) either 15 minutes before or 12 hours after bacterial inoculation. Reduces the ability of Rns and RegR to activate the transcription of their target promoters, but does not affect the activity of AggR and ToxT.

          Please note that the molecular weight for this compound is batch-specific due to variable water content.
          Catalogue Number530353
          Brand Family Calbiochem®
          Synonyms5-((4-Chlorobenzyl)sulfanyl)-1,3,4-thiadiazol-2-amine, Virulence Regulator RegA Inhibitor, NSC-523933
          References
          ReferencesYang, J., et al. 2013. J. Biol. Chem. 288, 31115.
          Product Information
          CAS number72836-33-0
          FormPale yellow powder
          Hill FormulaC₉H₈ClN₃S₂
          Chemical formulaC₉H₈ClN₃S₂
          ReversibleY
          Applications
          Biological Information
          Primary TargetRegA
          Purity≥95% by HPLC
          Physicochemical Information
          Cell permeableY
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Protect from Light Protect from light
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze 9-20°C). Stock solutions are stable for up to 6 months at -20°C.
          Packaging Information
          Packaged under inert gas Packaged under inert gas
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Regacin - CAS 72836-33-0 - Calbiochem SDS

          Title

          Safety Data Sheet (SDS) 

          References

          Reference overview
          Yang, J., et al. 2013. J. Biol. Chem. 288, 31115.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision28-April-2014 JSW
          Synonyms5-((4-Chlorobenzyl)sulfanyl)-1,3,4-thiadiazol-2-amine, Virulence Regulator RegA Inhibitor, NSC-523933
          DescriptionAn orally bioavailable, non-toxic, thio-thiadiazolamine compound that specifically inhibits the DNA-binding capacity of virulence regulator, RegA (IC50 = 1.7 µM in E. coli strain MC4100 (kfc-lacZ, pACYC184-regA), by interacting with amino acid residues within a conserved region of the DNA-binding domain, but without affecting RegA dimerization. Interacts with the double helix-turn-helix (HTH) domain that results in a loss of binding affinity of RegA for its DNA targets. This interaction appears to involve W188 and R223 residues that are conserved in several RegA homologs including ToxT, AggR, RegR, and Rns. Shown to be highly effective in reducing Citrobacter rodentium colonization when administered perorally to mice (~50 mg/kg) either 15 minutes before or 12 hours after bacterial inoculation. Reduces the ability of Rns and RegR to activate the transcription of their target promoters, but does not affect the activity of AggR and ToxT.
          FormPale yellow powder
          Intert gas (Yes/No) Packaged under inert gas
          CAS number72836-33-0
          Chemical formulaC₉H₈ClN₃S₂
          Purity≥95% by HPLC
          SolubilityDMSO (100 mg/ml)
          Storage +2°C to +8°C
          Protect from light
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze 9-20°C). Stock solutions are stable for up to 6 months at -20°C.
          Toxicity Standard Handling
          ReferencesYang, J., et al. 2013. J. Biol. Chem. 288, 31115.