SCM009 Rat Neural Stem Cell Expansion Medium

SCM009
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      Overview

      Replacement Information

      Key Specifications Table

      Key Applications
      Cult
      Description
      Catalogue NumberSCM009
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionRat Neural Stem Cell Expansion Medium
      OverviewThe Rat Neural Stem Cell Expansion Medium is provided as a two-component system that is convenient and easy to use. This product includes the Neural Stem Cell Basal Medium, a defined serum-free medium that has been optimized for the growth and in vitro differentiation of neural stem cells derived from rodents. A solution of basic FGF-2 growth factor is also provided separately, which when added to the Neural Stem Cell Basal Medium allows for the growth and proliferation of rat neural stem cells. Withdrawal of bFGF-2 from the Neural Stem Cell Basal Medium will result in the spontaneous differentiation of rat neural stem cells.
      Materials Required but Not Delivered• Cryopreserved Rat Cortical Neural Stem Cells (Millipore Catalog No. SCR022)
      • Neural Stem Cell Characterization Kit (Millipore Catalog No. SCR019)
      • Poly-L-ornithine (Sigma Catalog No. P3655)
      • Laminin (Millipore Catalog No. CC095)
      • Accutase solution (Millipore Catalog No. SCR005)
      References
      Product Information
      Components
      • Neural Stem Cell Basal Medium (Cat.# SCM003), 500 mL
      • bFGF, 10 ug
      Applications
      ApplicationThe Rat Neural Stem Cell Expansion Medium is provided as a two-component system that is convenient & easy to use.
      Key Applications
      • Stem Cell Culture
      Biological Information
      Media FormLiquid
      Stem Cell Type
      • Neural Stem Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEach lot of medium is tested for the optimal cell growth, proliferation and differentiation of mouse neural stem cells. Sterility Testing: Negative; Osmolarity: 299; pH: 7.4
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsPart number SCM003, the Neural Stem Cell Basal Medium should be stored at -20°C until ready to use. Upon thawing, the basal medium should be stored at 2-8°C for up to one month.


      FGF-2 (10 µg, lyophilized) should be reconstituted with 100 µL 5 mM Tris-HCL, pH 7.6 for a final concentration of 100 µg/mL. Dispense into aliquots to avoid repeated freeze-thaw. Store at -20°C for up to six months. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use. Multiple freeze/thaw cycles will result in significant loss of activity.
      Packaging Information
      Material Size1 kit
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Rat Neural Stem Cell Expansion Medium SDS

      Title

      Safety Data Sheet (SDS) 

      Rat Neural Stem Cell Expansion Medium Certificates of Analysis

      TitleLot Number
      RAT NEURAL STEM CELL EXPANSION MEDIUM2470941
      RAT NEURAL STEM CELL EXPANSION MEDIUM2485885
      RAT NEURAL STEM CELL EXPANSION MEDIUM2989616
      RAT NEURAL STEM CELL EXPANSION MEDIUM3116476
      RAT NEURAL STEM CELL EXPANSION MEDIUM - 21400142140014
      RAT NEURAL STEM CELL EXPANSION MEDIUM - 23905062390506
      RAT NEURAL STEM CELL EXPANSION MEDIUM - 24419372441937
      RAT NEURAL STEM CELL EXPANSION MEDIUM - 24501132450113
      RAT NEURAL STEM CELL EXPANSION MEDIUM - 19595801959580
      RAT NEURAL STEM CELL EXPANSION MEDIUM - 22271502227150

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      Catalog Number Description  
      SCM003 Neural Stem Cell Basal Medium Show Pricing & Availability

      Categories

      Life Science Research > Cell Culture and Systems > Cell Culture Media and Supplements > Stem Cell Culture Media