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      Replacement Information

      Key Specifications Table

      Analytes AvailableSpecies ReactivityKey Applications
      IL-6 R ELISA
      Catalogue NumberEZRIL6
      DescriptionRat IL-6 ELISA Kit, EZRIL6
      Background InformationInterleukin 6 (IL-6) is a prototypic member of the IL-6 superfamily of cytokines. It is secreted by a variety of lymphoid and non-lymphoid cells including: T cells, B cells, monocytes, fibroblasts, hepatocytes, endothelial cells and keratinocytes. Mature rat IL-6 is a 187 amino acid residue protein. At the protein sequence level, there is 39% identity between rat and human, and 87% identity between rat and mouse. The production of IL-6 can be induced by numerous signals, such as mitogenic or antigenic stimulation, lipopolysaccharides, calcium ionophores, cytokines and viruses.
      As a pleiotropic cytokine, IL-6 plays important roles in immune response, inflammation, hematopoiesis, bone metabolism, nerve cell functions and cancer progression. Elevated serum IL-6 levels have been observed in a number of pathological conditions, including bacterial and viral infections, trauma, autoimmune diseases, inflammation and malignancies.
      The Rat IL-6 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with a capture antibody. This kit is specifically designed for the accurate quantification of rat IL-6 from cell culture supernatant, serum, plasma and other biological fluids. It is analytically validated with ready-to-use reagents.
      Product Information
      Precision, %
      • Intra-assay: <10 %
      • Inter-assay: <15 %
      ApplicationThe Rat IL-6 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA) is designed for the accurate quantification of rat IL-6 from cell culture supernatant, serum, plasma & other biological fluids.
      Key Applications
      • ELISA
      Biological Information
      Specificity4.1% cross-reactivity was observed for mouse IL-6. No other cross-reactivity was observed when this kit was used to analyze the following recombinant cytokines/chemokines, each at a concentration of 50 ng/mL.

      Human: IL-6

      Rat: IL-1a, IL-1b, IL-2, IL-4, IL-5, IL-10, IL-12, IL-13, IL-17A, IL-17F, IL-18, IL-22, IL-23, IL-33, CCL20, GM-CSF, IFN-g, KC, MCP-1, RANTES, TNF-a
      Species Reactivity
      • Rat
      Analytes Available
      • IL-6
      • 96%
      Physicochemical Information
      • 5.3 pg/mL
      Linearity of DilutionContact Technical Service for this information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size96-well strip plate
      Transport Information
      Supplemental Information



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      Rat IL-6 ELISA Kit