553210 Rapamycin - CAS 53123-88-9 - Calbiochem

553210
  
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      Overview

      Replacement Information

      Key Specifications Table

      Empirical FormulaCAS #
      C₅₁H₇₉NO₁₃ 53123-88-9
      Description
      OverviewAnti-fungal and immunosuppressant. Selectively inhibits the mammalian target of rapamycin (mTOR) and blocks the subsequent activation of p70 S6 kinase (IC50 = 50 pM). Specifically inhibits mTORC1, but mTORC2 that phosphorylates Akt at Ser473 appears to be insensitive to rapamycin. Prevents the translational activation of IGF-II. Also prevents resting T-cells from entering the cell cycle, but does not directly arrest cell cycle progression. Shown to inhibit later signaling events, such as p110Rb phosphorylation, p34cdc2 kinase activation, and cyclin A synthesis. Exhibits strong binding to FK-506 binding proteins. Also reported to induce apoptosis in a murine B-cell line, to inhibit lymphokine-induced cell proliferation at the G1 phase, and to irreversibly arrest Saccharomyces cerevisiae cells in the G1 phase. A 5 mM (500 µg/109 µl) solution of Rapamycin (Cat. No. 553211) in DMSO and a 10 mM (1 mg/109 µl) solution of Rapamycin (Cat. No. 553212) in EtOH is also available.
      Catalogue Number553210
      Brand Family Calbiochem®
      SynonymsmTOR Inhibitor I
      References
      ReferencesChen, T., et al. 2011. Aging Cell. 10, 908.
      Powell, D.J., et al. 2003. Mol. cell Biol. 23, 7794.
      Braun, W., et al. 1995. FASEB J. 9, 63.
      Nielsen, F.C., et al. 1995. Nature 377, 358.
      Aagaard-Tillery, K.M. and Jelinek, D.F. 1994. Cell. Immunol. 156, 493.
      Gottschalk, A.R., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 7350.
      Morice, W.G., et al. 1993. J. Biol. Chem. 268, 3734.
      Terada, N., et al. 1993. J. Biol. Chem. 268, 12062.
      Kuo, J., et al. 1992. Nature 358, 70.
      Price, D.J., et al. 1992. Science 257, 973.
      Heitman, J., et al. 1991. Science 253, 905.
      Kay, J.E., et al. 1991. Immunology 72, 544.
      Schreiber, S.L. 1991. Science 251, 283.
      Bierer, B.E., et al. 1990. Proc. Natl. Acad. Sci. USA 87, 9231.
      Dumont, F.J., et al. 1990. J. Immunol. 144, 251.
      Product Information
      CAS number53123-88-9
      ATP CompetitiveN
      FormClear film
      Hill FormulaC₅₁H₇₉NO₁₃
      Chemical formulaC₅₁H₇₉NO₁₃
      ReversibleN
      Structure formula ImageStructure formula Image
      Applications
      Biological Information
      Primary TargetMammalian target of rapamycin (mTOR)
      Primary Target IC<sub>50</sub>50 pM against p70 S6 kinase
      Purity≥99% by HPLC
      Physicochemical Information
      Cell permeableN
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      RTECSVE6250000
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Rapamycin - CAS 53123-88-9 - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      Rapamycin - CAS 53123-88-9 - Calbiochem Certificates of Analysis

      TitleLot Number
      553210

      References

      Reference overview
      Chen, T., et al. 2011. Aging Cell. 10, 908.
      Powell, D.J., et al. 2003. Mol. cell Biol. 23, 7794.
      Braun, W., et al. 1995. FASEB J. 9, 63.
      Nielsen, F.C., et al. 1995. Nature 377, 358.
      Aagaard-Tillery, K.M. and Jelinek, D.F. 1994. Cell. Immunol. 156, 493.
      Gottschalk, A.R., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 7350.
      Morice, W.G., et al. 1993. J. Biol. Chem. 268, 3734.
      Terada, N., et al. 1993. J. Biol. Chem. 268, 12062.
      Kuo, J., et al. 1992. Nature 358, 70.
      Price, D.J., et al. 1992. Science 257, 973.
      Heitman, J., et al. 1991. Science 253, 905.
      Kay, J.E., et al. 1991. Immunology 72, 544.
      Schreiber, S.L. 1991. Science 251, 283.
      Bierer, B.E., et al. 1990. Proc. Natl. Acad. Sci. USA 87, 9231.
      Dumont, F.J., et al. 1990. J. Immunol. 144, 251.

      Citations

      Title
    • Chen, T., et al. 2011. Aging Cell. 10, 908.
    • Yu, YL., et al. 2005. Journal of Biological Chemistry 280, 29533.
    • Vega, R.B., et al. 2004. Molecular and Cellular Biology 24, 8374.
    • Liao, X., et al. 2003. Endocrinology 144, 1656.
    • Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision11-September-2014 JSW
      SynonymsmTOR Inhibitor I
      DescriptionSelectively inhibits the mammalian target of rapamycin (mTOR) and blocks the subsequent activation of p70 S6 kinase (IC50 = 50 pM). Specifically inhibits mTORC1, but mTORC2 that phosphorylates Akt at Ser473 appears to be insensitive to rapamycin. Prevents the translational activation of IGF-II. Also prevents resting T-cells from entering the cell cycle, but does not directly arrest cell cycle progression. Shown to inhibit later signaling events, such as p110Rb phosphorylation, p34cdc2 kinase activation, and cyclin A synthesis. Exhibits strong binding to FK-506 binding proteins. Also reported to induce apoptosis in a murine B-cell line, to inhibit lymphokine-induced cell proliferation at the G1 phase, and to irreversibly arrest Saccharomyces cerevisiae cells in the G1 phase.
      FormClear film
      CAS number53123-88-9
      RTECSVE6250000
      Chemical formulaC₅₁H₇₉NO₁₃
      Structure formulaStructure formula
      Purity≥99% by HPLC
      SolubilityDMSO (200 mg/ml) or Ethanol (50 mg/ml)
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Toxicity Standard Handling
      Merck USA index14, 8114
      ReferencesChen, T., et al. 2011. Aging Cell. 10, 908.
      Powell, D.J., et al. 2003. Mol. cell Biol. 23, 7794.
      Braun, W., et al. 1995. FASEB J. 9, 63.
      Nielsen, F.C., et al. 1995. Nature 377, 358.
      Aagaard-Tillery, K.M. and Jelinek, D.F. 1994. Cell. Immunol. 156, 493.
      Gottschalk, A.R., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 7350.
      Morice, W.G., et al. 1993. J. Biol. Chem. 268, 3734.
      Terada, N., et al. 1993. J. Biol. Chem. 268, 12062.
      Kuo, J., et al. 1992. Nature 358, 70.
      Price, D.J., et al. 1992. Science 257, 973.
      Heitman, J., et al. 1991. Science 253, 905.
      Kay, J.E., et al. 1991. Immunology 72, 544.
      Schreiber, S.L. 1991. Science 251, 283.
      Bierer, B.E., et al. 1990. Proc. Natl. Acad. Sci. USA 87, 9231.
      Dumont, F.J., et al. 1990. J. Immunol. 144, 251.
      Citation
    • Chen, T., et al. 2011. Aging Cell. 10, 908.
    • Yu, YL., et al. 2005. Journal of Biological Chemistry 280, 29533.
    • Vega, R.B., et al. 2004. Molecular and Cellular Biology 24, 8374.
    • Liao, X., et al. 2003. Endocrinology 144, 1656.