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17-321 | Rap1 Activation Assay Kit

17-321
1 kit  Kit capacity: 20-30 assays
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      Overview

      Replacement Information

      Key Specifications Table

      Key Applications
      ABA
      Description
      Catalogue Number17-321
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionRap1 Activation Assay Kit
      References
      Product Information
      Components
      • 100X GTPγS, 10mM (Cat.# 20-176)
      • 100X GDP, 100mM (Cat.# 20-177)
      • Rap1 Activation Lysis Buffer, 2X
      • Anti-Rap1
      • Rap1 Assay Reagent (Ral GDS-RBD, agarose) (Cat.# 14-455)
      Applications
      ApplicationNon-radioactive Rap1 Activation Assay Kit that uses Ral GDS RBD, agarose (Catalog # 14-455) to precipitate Rap1-GTP from cell lysates & detection by a Rap1 specific antibody.
      Key Applications
      • Affinity Binding Assay
      Biological Information
      Entrez Gene Number
      Gene Symbol
      • RAP1GDS1
      • MGC118859
      • GDS1
      • RAP1
      • MGC118861
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P52306 # Stimulates GDP/GTP exchange reaction of a group of small GTP-binding proteins (G proteins) including Rap1a/Rap1b, RhoA, RhoB and KRas, by stimulating the dissociation of GDP from and the subsequent binding of GTP to each small G protein.
      SIZE: 607 amino acids; 66403 Da
      SIMILARITY: SwissProt: P52306 ## Contains 5 ARM repeats.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by affinity precipitation assay. Ral GDS RBD, agarose precipitated GTP-Rap1 from HEK293 lysates. The precipitated GTP-Rap1 was detected by immunoblot analysis using Anti-Rap1.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size1 kit
      Material PackageKit capacity: 20-30 assays
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Rap1 Activation Assay Kit - 2089003 2089003
      Rap1 Activation Assay Kit - 2140555 2140555
      Rap1 Activation Assay Kit - 2359951 2359951
      Rap1 Activation Assay Kit - 2388888 2388888
      Rap1 Activation Assay Kit - 2420450 2420450
      Rap1 Activation Assay Kit - 2464565 2464565
      Rap1 Activation Assay Kit - 0605028921 0605028921
      Rap1 Activation Assay Kit - 0609040114 0609040114
      Rap1 Activation Assay Kit - 0701049372 0701049372
      Rap1 Activation Assay Kit - 2003356 2003356
      Rap1 Activation Assay Kit - 2023249 2023249
      Rap1 Activation Assay Kit - 2055995 2055995
      Rap1 Activation Assay Kit - 2069517 2069517
      Rap1 Activation Assay Kit - 2194177 2194177
      Rap1 Activation Assay Kit - 22319 22319
      Rap1 Activation Assay Kit - 25944 25944
      Rap1 Activation Assay Kit - 26252 26252
      Rap1 Activation Assay Kit - 26545 26545
      Rap1 Activation Assay Kit - 30114 30114
      Rap1 Activation Assay Kit - A701049372 A701049372
      Rap1 Activation Assay Kit - DAM1524119 DAM1524119
      Rap1 Activation Assay Kit - DAM1588150 DAM1588150
      Rap1 Activation Assay Kit - DAM1658633 DAM1658633
      Rap1 Activation Assay Kit - DAM1708215 DAM1708215
      Rap1 Activation Assay Kit - DAM1745419 DAM1745419
      Rap1 Activation Assay Kit - DAM1778059 DAM1778059
      Rap1 Activation Assay Kit - DAM1813271 DAM1813271
      Rap1 Activation Assay Kit - JBC1838320 JBC1838320
      Rap1 Activation Assay Kit - JBC1872249 JBC1872249
      Rap1 Activation Assay Kit - JBC1924366 JBC1924366
      Rap1 Activation Assay Kit -2502995 2502995
      Rap1 Activation Assay Kit -2581744 2581744
      Rap1 Activation Assay Kit -2651897 2651897
      Rap1 Activation Assay Kit -2676610 2676610
      Rap1 Activation Assay Kit -2691240 2691240
      Rap1 Activation Assay Kit -2730943 2730943
      Rap1 Activation Assay Kit -2752627 2752627
      Rap1 Activation Assay Kit -2803321 2803321
      Rap1 Activation Assay Kit -2811962 2811962

      References

      Reference overviewPub Med ID
      Measurement of GTP-bound Ras-like GTPases by activation-specific probes.
      van Triest, M, et al.
      Meth. Enzymol., 333: 343-8 (2001) 2001

      11400350 11400350
      Signaling of hepatocyte growth factor/scatter factor (HGF) to the small GTPase Rap1 via the large docking protein Gab1 and the adapter protein CRKL.
      Sakkab, D, et al.
      J. Biol. Chem., 275: 10772-8 (2000) 2000

      Show Abstract
      10753869 10753869
      Identification of guanine nucleotide exchange factors (GEFs) for the Rap1 GTPase. Regulation of MR-GEF by M-Ras-GTP interaction
      Rebhun, J F, et al
      J Biol Chem, 275:34901-8 (2000) 2000

      10934204 10934204
      Rapid Ca2+-mediated activation of Rap1 in human platelets.
      Franke, B, et al.
      EMBO J., 16: 252-9 (1997) 1997

      Show Abstract
      9029146 9029146
      Differential interaction of the ras family GTP-binding proteins H-Ras, Rap1A, and R-Ras with the putative effector molecules Raf kinase and Ral-guanine nucleotide exchange factor
      Herrmann, C, et al
      J Biol Chem, 271:6794-800 (1996) 1996

      8636102 8636102

      Brochure

      Title
      Hallmarks of Aging (EMD)

      Related Products & Applications

      Kit Component

      Catalog Number Description  
      14-455 Rap1 Assay Reagent (Ral GDS-RBD, agarose), 650 µg Show Pricing & Availability
      20-176 100X GTPγS, 10mM Show Pricing & Availability
      20-177 100X GDP, 100mM Show Pricing & Availability

      Categories

      Life Science Research > Protein Detection and Quantification > Activity Assays > Activation Assays