03-114 RIPAb+ Musashi 1 - RIP Validated Antibody and Primer Set, rabbit monoclonal

03-114
10 assays  10 assays per set. Recommended use: ~5 μL of antibody per RIP (dependent upon biological context).
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey Applications
      H, R RIP, WB, FC, ICC, IP, IH(P)
      Description
      Catalogue Number03-114
      Brand Family Upstate
      Trade Name
      • RIPAb+
      • Upstate
      DescriptionRIPAb+ Musashi 1 - RIP Validated Antibody and Primer Set, rabbit monoclonal
      OverviewRIPAb+ antibodies are evaluated using the RNA Binding Protein Immunoprecipitation (RIP) assay. Each RIPAb+ antibody set includes a negative control antibody to ensure specificity of the RIP reaction and is verified for the co-immunoprecipitation of RNA associated specifically with the immunoprecipitated RNA binding protein of interest. Where appropriate, the RIPAb+ set also includes quantitative RT-PCR control primers (RIP Primers) to biologically validate your IP results by successfully co-precipitating the specific RNA targets, such as messenger RNAs. The qPCR protocol and primer sequences are provided, allowing researchers to validate RIP protocols when using the antibody in their experimental context. If a target specific assay is not provided, the RIPAb+ kit is validated using an automated microfluidics-based assay by enrichment of detectable RNA over control immunoprecipitation.
      Alternate Names
      • Musashi (Drosophila) homolog 1
      • musashi 1
      • musashi homolog 1 (Drosophila)
      Background InformationIn mammals, the Musashi family is important for cell fate determination, playing roles in maintenance of the stem-cell state, differentiation and tumorigenesis. Musashi-1 (also known as Msi1), is selectivley expressed in neural progenitor cells, including neural stem cells. Outside the nervous system, Musashi-1 is a selective marker for intestinal stem or early lineage cells. Musashi-1 interacts with the Notch pathway during asymmetric cell division by binding the 3' UTR of Numb. Numb is prevented from repressing Notch signaling when Musashi-1 is present.
      References
      Product Information
      FormatUnpurified
      Control
      • Includes negative control rabbit IgG antibody and control primers specific for human NUMB.
      PresentationAnti-Musashi 1 (Rabbit Monoclonal). One vial containing 50 μL of Rabbit Monoclonal Antibody in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Store at -20°C.

      Normal Rabbit IgG. One vial containing 125 μg of Rabbit IgG in 125 μL of storage buffer containing 0.05% sodium azide. Store at -20°C.

      RIP Primers, NUMB. One vial containing 75 μL of 5 μM of each primer specific for human NUMB. Store at -20°C.
      FOR: AGC CAG CCC ATA CTG CTC TA
      REV: CGG ACG CTC TTA GAC ACC TC
      Applications
      ApplicationThis RIPAb+ Musashi 1 -RIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
      Key Applications
      • RNA Binding Protein Immunoprecipitation (RIP)
      • Western Blotting
      • Flow Cytometry
      • Immunocytochemistry
      • Immunoprecipitation
      • Immunohistochemistry (Paraffin)
      Application NotesImmunoprecipitation from RIP lysate:
      Representative lot data.
      RIP lysate from SHSH-5Y cells (2 X 10E6 cell equivalents per IP) was subjected to immunoprecipitation using 0.5 µL of either a normal rabbit IgG or 0.5 µL of Anti-Musashi 1 antibody.
      Precipitated proteins (lane 1: rabbit IgG, lane 2: anti-Musashi 1) and SHSH-5Y whole cell lysate (lane 3) were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Musashi 1 antibody (1:2,000 dilution). Proteins were visualized using One-Step™ IP-Western kit (GenScript).
      Arrow indicates Musashi 1 (Please see figures).

      Immunocytochemistry Analysis:
      Representative lot data.
      A previous lot of this antibody was used in immunofluorescent staining of PC12 cells using Anti-Musashi 1 (Please see figures).

      Immunohistochemistry (Paraffin) Analysis:
      Representative lot data.
      A previous lot of this antibody was used in immunohistochemical staining of paraffin-embedded human brain using Anti-Musashi 1 (Please see figures).

      Western Blot Analysis:
      Representative lot data.
      SHSY5Y cell lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with Anti-Musashi 1 (1:2,000 dilution).
      Proteins were visualized using a Goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
      Arrow indicates Musashi 1 (~39 kDa) (Please see figures).


      Flow Cytometry:
      A 1:40 dilution of a previous lot was used in flow cytometry.
      Biological Information
      ImmunogenSynthetic peptide corresponding to residues on the N-terminus of human Musashi-1.
      EpitopeN-terminus
      HostRabbit
      SpecificityRecognizes Musashi 1 at the N-terminus.
      IsotypeIgG
      Species Reactivity
      • Human
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a protein containing two conserved tandem RNA recognition motifs. Similar proteins in other species function as RNA-binding proteins and play central roles in posttranscriptional gene regulation. Expression of this gene has been correlated with the grade of the malignancy and proliferative activity in gliomas and melanomas. A pseudogene for this gene is located on chromosome 11q13.
      Gene Symbol
      • MSI1
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U1-3AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system By similarity.
      SUBCELLULAR LOCATION: Cytoplasm By similarity. Nucleus By similarity.
      TISSUE SPECIFICITY: Detected in fetal kidney, brain, liver and lung, and in adult brain and pancreas. Detected in hepatoma cell lines.
      DOMAIN: The first RNA recognition motif binds more strongly to RNA compared to the second one By similarity.
      SEQUENCE SIMILARITIES: Belongs to the Musashi family.
      Contains 2 RRM (RNA recognition motif) domains.
      Molecular Weight~39 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRNA Binding Protein Immunoprecipitation:
      RIP Lysate prepared from SHSH-5Y cells (2 X 10E7 cell equivalents per IP) were subjected to immunoprecipitation using 5 µL of either a normal rabbit IgG or 5 µL of Anti-Musashi 1 antibody and the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Cat. # 17-700).
      Successful immunoprecipitation of Musashi 1-associated RNA was verified by qPCR using RIP Primers NUMB (Please see figures).
      Please refer to the Magna RIP™ (Cat. # 17-700) or EZ-Magna RIP™ (Cat. # 17-701) protocol for experimental details.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size10 assays
      Material Package10 assays per set. Recommended use: ~5 μL of antibody per RIP (dependent upon biological context).
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      RIPAb+ Musashi 1 - 20803222080322
      RIPAb+ Musashi 1 - 22687902268790
      RIPAb+ Musashi 1 - NRG1761581NRG1761581

      References

      Reference overviewPub Med ID
      Increased particulate phosphodiesterase 4 in the prefrontal cortex supports 5-HT4 receptor-induced improvement of object recognition memory in the rat.
      Guénaëlle Levallet,Maïté Hotte,Michel Boulouard,François Dauphin
      Psychopharmacology  202  2009

      Show Abstract
      18712363 18712363
      Inhibition of Bacillus subtilis and Listeria innocua by nisin in combination with some naturally occurring organic compounds.
      N A Olasupo,D J Fitzgerald,A Narbad,M J Gasson
      Journal of food protection  67  2004

      Show Abstract
      15035380 15035380

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      Categories

      Life Science Research > Protein Detection and Quantification > Immunoassays > Immunoprecipitation (IP) > RNA-Binding Protein Immunoprecipitation (RIP) > RIP Validated Antibodies
      Life Science Research > Antibodies and Assays > Immunoassays > Immunoprecipitation (IP) > RNA-Binding Protein Immunoprecipitation (RIP) > RIP Validated Antibodies
      Life Science Research > Antibodies and Assays > Primary Antibodies