544049 Pyrintegrin - Calbiochem

544049
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service
      View Pricing & Availability

      Overview

      Replacement Information

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      544049-5MG
      Retrieving availability...
      Limited AvailabilityLimited Availability
      Stocked 
      Discontinued
      Limited Quantities Available
      Available
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service
          Contact Customer Service

          Glass bottle 5 mg
          Retrieving price...
          Price could not be retrieved
          Minimum Quantity needs to be mulitiple of
          Upon Order Completion More Information
          You Saved ()
           
          Request Pricing
          Description
          OverviewA cell-permeable pyrimidine compound that promotes the adhesion of individually dissociated hESCs on matrigel- or laminin-, but not gelatin-coated surfaces and substantially reduces trypsinization-induced apoptosis (10% vs 81% TUNEL-positive cells from 2 µM Ptn or DMSO control matrigel cultures, respectively, after trypsinization), while maintaining cells’ self-renewal ability (colony formation rate = 25% or 1% 4 d after low density seeding of single-cells on matrigel surface with 2 µM Ptn or DMSO control, respectively) and the pluripotency of expanded cultures for more than 40 passages. Ptn treatment is shown to induce the activation of β1 integrin and multiple growth factor receptors, including FGFR1, IGFR1, EGFR1, and ErbB2, while blockage of β1 integrin, PI 3-K, or growth factor receptors abolishes the survival promoting effect of Ptn. Single-cell dissociation is demonstrated to hyperactivate Rho-ROCK signaling, while efficient hESC-ECM interaction suppresses Rho-Rock activation, resulting in the stabilization of newly synthesized cell surface E-cadherin, which in turn promotes cell-cell adhesion, an important determinant of hESC survival.
          Catalogue Number544049
          Brand Family Calbiochem®
          SynonymsPtn, N-(Cyclopropylmethyl)-4-(4-(6-hydroxy-3,4-dihydroquinolin-1(2H)-yl)pyrimidin-2-ylamino)benzenesulfonamide, β1 Integrin Activator
          References
          ReferencesXu, Y., et al. 2010. Proc. Natl. Acad. Sci. USA 107, 8129.
          Product Information
          FormWhite solid
          Hill FormulaC₂₃H₂₅N₅O₃S
          Chemical formulaC₂₃H₂₅N₅O₃S
          Structure formula ImageStructure formula Image
          Applications
          Biological Information
          Purity≥95% by HPLC
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Protect from Light Protect from light
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
          Packaging Information
          Packaged under inert gas Packaged under inert gas
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Pyrintegrin - Calbiochem SDS

          Title

          Safety Data Sheet (SDS) 

          Pyrintegrin - Calbiochem Certificates of Analysis

          TitleLot Number
          544049

          References

          Reference overview
          Xu, Y., et al. 2010. Proc. Natl. Acad. Sci. USA 107, 8129.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision31-August-2011 RFH
          SynonymsPtn, N-(Cyclopropylmethyl)-4-(4-(6-hydroxy-3,4-dihydroquinolin-1(2H)-yl)pyrimidin-2-ylamino)benzenesulfonamide, β1 Integrin Activator
          DescriptionA cell-permeable pyrimidine compound that promotes the adhesion of individually dissociated hESCs on matrigel- or laminin-, but not gelatin-coated surfaces and substantially reduces trypsinization-induced apoptosis (10% vs 81% TUNEL-positive cells from 2 µM Ptn or DMSO control matrigel cultures, respectively, after trypsinization), while maintaining cells' self-renewal ability (colony formation rate = 25% or 1% 4 d after low density seeding of single-cells on matrigel surface with 2 µM Ptn or DMSO control, respectively) and the pluripotency of expanded cultures for more than 40 passages. Ptn treatment is shown to induce the activation of β1 integrin and multiple growth factor receptors, including FGFR1, IGFR1, EGFR1, and ErbB2, while blockage of β1 integrin, PI 3-K, or growth factor receptors abolishes the survival promoting effect of Ptn. Single-cell dissociation is demonstrated to hyperactivate Rho-ROCK signaling, while efficient hESC-ECM interaction suppresses Rho-Rock activation, resulting in the stabilization of newly synthesized cell surface E-cadherin, which in turn promotes cell-cell adhesion, an important determinant of hESC survival.
          FormWhite solid
          Intert gas (Yes/No) Packaged under inert gas
          Chemical formulaC₂₃H₂₅N₅O₃S
          Structure formulaStructure formula
          Purity≥95% by HPLC
          SolubilityDMSO (100 mg/ml)
          Storage +2°C to +8°C
          Protect from light
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
          Toxicity Standard Handling
          ReferencesXu, Y., et al. 2010. Proc. Natl. Acad. Sci. USA 107, 8129.