71772 ProteoExtract® Transmembrane Protein Extraction Kit

71772
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      Overview

      Replacement Information

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      71772-3
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          20 rxn
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          Description
          OverviewThe ProteoExtract® Transmembrane Protein Extraction Kit (TM-PEK) provides a detergent-free novel chemistry to enable the mild and efficient extraction of transmembrane proteins, such as GPCRs, from mammalian cells and tissues. The fraction enriched in membrane proteins and large protein complexes is directly compatible with enzyme assays, non-denaturing and SDS-based gel electrophoresis, Western blotting and immunoprecipitation. The enriched fraction is appropriate for mass spec-based analysis after a tryptic in-gel digest. The kit contains two different extraction reagents for protein-dependent optimization, protease inhibitor cocktail, and appropriate buffers for processing twenty samples (2-5 x 106 cells or 25-50 mg tissue per sample).
          Catalogue Number71772
          Brand Family Novagen®
          References
          ReferencesNakamura, E., et al. 2008. Cancer Sci. 99, 1390.
          Product Information
          Applications
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          R PhraseR: 25-33

          Toxic if swallowed.
          Danger of cumulative effects.
          S PhraseS: 37-45

          Wear suitable gloves.
          In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Toxic
          Storage +2°C to +8°C
          Do not freeze Ok to freeze
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          ProteoExtract® Transmembrane Protein Extraction Kit SDS

          Title

          Safety Data Sheet (SDS) 

          ProteoExtract® Transmembrane Protein Extraction Kit Certificates of Analysis

          TitleLot Number
          71772

          References

          Reference overview
          Nakamura, E., et al. 2008. Cancer Sci. 99, 1390.

          Brochure

          Title
          Protein Purification and Preparation

          Technical Info

          Title
          Western Blotting Quick Reference Product Guide

          Citations

          Title
        • Waetzig G.H., et al. 2010. The Journal of Biological Chemistry 285, 1781.
        • Ernst, C.M., et al. 2009. doi: 10.1371/journal.ppat.1000660
        • User Protocols

          Title
          TB477