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539480 | Proteinase K, Tritirachium album

539480
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      539480-100MG
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          Glass bottle 100 mg
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          539480-25MG
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              Glass bottle 25 mg
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              Description
              OverviewSerine protease that exhibits strong proteolytic activity on a wide variety of denatured and native proteins of high molecular weight. Because of its rapid proteolytic inactivation of endogenous nucleases, it can be used for isolation of mRNA and high molecular weight DNA. Preferentially cleaves bonds next to the carbonyl group of N-substituted hydrophobic, aliphatic, and aromatic amino acids. Inhibited by DFP, Hg2+, and PMSF. Not inactivated by metal chelators, sulfhydryl reagents, TLCK, or TPCK.
              Catalogue Number539480
              Brand Family Calbiochem®
              References
              ReferencesWatazu, Y., et al. 1993. J. Lab. Clin. Anal. 7, 81.
              Lebherz, H.G., et al. 1986. Biochem. J. 233, 51.
              Ebeling, W., et al. 1974. Eur. J. Biochem. 47, 91.
              Product Information
              CAS number39450-01-6
              Activity≥30 mAnsonU/mg dry weight
              Unit of DefinitionOne mAnson unit is defined as the amount of enzyme that will liberate 1.0 µmol of Folin-positive amino acids (calculated as tyrosine) per min at 37°C, pH 7.5.
              EC number3.4.21.14
              FormWhite lyophilized solid
              FormulationLyophilized from calcium acetate
              Applications
              Biological Information
              Specific Activity≥40 mAnsonU/mg protein
              Physicochemical Information
              ContaminantsDNase: no detectable nicking activity with pBR322, incubation for 6 h at 37°C; RNase: no ribonuclease activity with MS2 RNA detected after incubation for 6 h at 37°C
              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              Safety Information
              Product Usage Statements
              Storage and Shipping Information
              Ship Code Ambient Temperature Only
              Toxicity Irritant
              Storage +2°C to +8°C
              Do not freeze Ok to freeze
              Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Stock solutions prepared in 50 mM Tris-HCl, 2 mM calcium acetate, pH 8.0 are stable for up to 1 year at 4°C.
              Packaging Information
              Transport Information
              Supplemental Information
              Specifications

              Documentation

              SDS

              Title

              Safety Data Sheet (SDS) 

              Certificates of Analysis

              TitleLot Number
              539480

              References

              Reference overview
              Watazu, Y., et al. 1993. J. Lab. Clin. Anal. 7, 81.
              Lebherz, H.G., et al. 1986. Biochem. J. 233, 51.
              Ebeling, W., et al. 1974. Eur. J. Biochem. 47, 91.

              Brochure

              Title
              Proteases Technical Bulletin
              Data Sheet

              Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

              Revision16-November-2016 JSW
              DescriptionSerine protease that exhibits strong proteolytic activity on a wide variety of denatured and native proteins of high molecular weight. Because of its rapid proteolytic inactivation of endogenous nucleases, it can be used for isolation of mRNA and high molecular weight DNA. Preferentially cleaves bonds next to the carbonyl group of N-substituted hydrophobic, aliphatic, and aromatic amino acids. Inhibited by DFP, Hg2+, and PMSF. Not inactivated by metal chelators, sulfhydryl reagents, TLCK, or TPCK.
              FormWhite lyophilized solid
              FormulationLyophilized from calcium acetate
              Recommended reaction conditions

              Anson Enzyme Activity Assay: Materials Required • 5 ml of Hemoglobin (20 mg/ml) in 100 mM phosphate buffer, pH 7.5 (6 M urea, 5 mM NaCl). • 0.6 ml of 20 mM CaCl2 dissolved in distilled water. • 0.1 ml of proteinase K (40 µg/ml) in 20 mM CaCl2 Activity Assay Protocol 1. Mix the 5 ml hemoglobin, 0.6 ml CaCl2, and 0.1 ml proteinase K and make up the volume to 6 ml with 20 mM CaCl2; incubate for 10 min at 25°C. 2. Add 10 ml 300 mM Trichloroacetic acid and incubate at room temperature for 30 min. 3. Centrifuge and transfer 5 ml of the supernatant to a clean tube. Add 10 ml 500 mM NaOH and 3 ml Folin reagent (diluted 1:2 with redistilled water); incubate for 5 min at room temperature. 4. Measure the ΔA at 750 nm. For calibration use tyrosine solutions. 1 mAnson unit liberates 1 µmole of Folin-positive amino acids per min (calculated as tyrosine) under test conditions.
              CAS number39450-01-6
              EC number3.4.21.14
              ContaminantsDNase: no detectable nicking activity with pBR322, incubation for 6 h at 37°C; RNase: no ribonuclease activity with MS2 RNA detected after incubation for 6 h at 37°C
              Specific activity≥40 mAnsonU/mg protein
              Activity≥30 mAnsonU/mg dry weight
              Unit definitionOne mAnson unit is defined as the amount of enzyme that will liberate 1.0 µmol of Folin-positive amino acids (calculated as tyrosine) per min at 37°C, pH 7.5.
              Solubility50 mM Tris-HCl, 2 mM calcium acetate, pH 8.0
              Storage +2°C to +8°C
              Do Not Freeze Ok to freeze
              Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Stock solutions prepared in 50 mM Tris-HCl, 2 mM calcium acetate, pH 8.0 are stable for up to 1 year at 4°C.
              Toxicity Irritant
              ReferencesWatazu, Y., et al. 1993. J. Lab. Clin. Anal. 7, 81.
              Lebherz, H.G., et al. 1986. Biochem. J. 233, 51.
              Ebeling, W., et al. 1974. Eur. J. Biochem. 47, 91.