539305 Protein G, Alkaline Phosphatase Conjugate

539305
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      539305-500UG
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          Plastic ampoule 500 μg
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          Description
          OverviewProtein G conjugated to alkaline phosphatase. Does not react with IgA, IgE, or IgM.
          Catalogue Number539305
          Brand Family Calbiochem®
          References
          Product Information
          FormLiquid
          FormulationIn 50 mM Tris buffer, 1 mM MgCl₂, 0.1 mM ZnCl₂, 1% BSA, 0.1% sodium azide, pH 7.6.
          Preservative≤0.1% sodium azide
          Applications
          Application NotesDirect Immunoassay (1:2500)
          Application Commentsp-Nitrophenyl phosphate, 1.0 mg/ml in 10% diethanolamine, pH 9.8, 1 mM MgCl2 at 25°C, was used as substrate to test immunoenzyme reactivity. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          PuritySingle band at 33.8 kDa by SDS-PAGE
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Yes
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Protein G, Alkaline Phosphatase Conjugate SDS

          Title

          Safety Data Sheet (SDS) 

          Protein G, Alkaline Phosphatase Conjugate Certificates of Analysis

          TitleLot Number
          539305
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision06-April-2009 RFH
          ApplicationDirect Immunoassay (1:2500)
          DescriptionRecombinant protein G, a cell-surface protein of Streptococcus species, binds to the Fc portions of immunoglobulins from various species. Like protein A, it is a versatile and economical means to isolate, detect and purify antibodies produced by many animals. It is especially valued because of its ability to react with human IgG3 and a number of animal immunoglobulins with which protein A does not react well (i.e., bovine goat, horse, rat and sheep). It also reacts more strongly with mouse immunoglobulins than protein A. Additionally, unlike protein A, it does not react with IgA, IgE, and IgM. Useful for detection of primary antibodies by various immunoassay methods at an average dilution of 1:2500.
          FormLiquid
          FormulationIn 50 mM Tris buffer, 1 mM MgCl₂, 0.1 mM ZnCl₂, 1% BSA, 0.1% sodium azide, pH 7.6.
          Concentration Label Please refer to vial label for lot-specific concentration
          PuritySingle band at 33.8 kDa by SDS-PAGE
          Preservative≤0.1% sodium azide
          Commentsp-Nitrophenyl phosphate, 1.0 mg/ml in 10% diethanolamine, pH 9.8, 1 mM MgCl2 at 25°C, was used as substrate to test immunoenzyme reactivity. Variables associated with assay conditions will dictate the proper working dilution.
          Storage +2°C to +8°C
          Do Not Freeze Yes
          Toxicity Standard Handling