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16-201 | Protein G Agarose/Salmon Sperm DNA, 2.5 mL

16-201
2.5 mL  2.5 mL packed beads provided as a 50% gel slurry for a final volume of 5 mL.
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      Overview

      Replacement Information

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      Achieve first time ChIP success with EZ-ChIP Assays
      Description
      Catalogue Number16-201
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionProtein G Agarose/Salmon Sperm DNA, 2.5 mL
      OverviewRecombinant Protein G covalently bound to agarose by cyanogen bromide linkage. Sonicated salmon sperm DNA blocks non-specific DNA binding sites on protein G agarose when collecting immunocomplexes for chromatin immunoprecipitations (ChIP assays).
      Alternate Names
      • ChIP agarose beads
      • ChIP agaraose G beads
      Background InformationRecombinant protein G has a very high affinity for the Fc regions of IgG molecules. Immoblisation of protein G on beads creates an affinity resin which can be used to purify IgG fractions from crude serum, ascites fluid or cell cuture media.
      References
      Product Information
      Presentation2.5 mL packed beads containing 1 mg sonicated salmon sperm DNA, 2.5 mg BSA and approximately 5 mg recombinant Protein G. Provided as a 50% gel slurry for a final volume of 5 mL per vial. Suspended in 10 mM Tris-HCl, 1 mM EDTA, pH 8.0, containing 0.05% sodium azide. Liquid suspension.
      Applications
      ApplicationRecombinant Protein G covalently bound to agarose beads by cyanogen bromide linkage for use in chromatin immunoprecipitations (ChIP assays). Sonicated salmon sperm DNA is included to block non-specific DNA binding sites on agarose beads. Every lot qualified using an Acetyl Histone H3 ChIP assay.
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated in Chromatin Immunoprecipitation for use to pre-clear the supernatant from sodium butyrate treated HeLa cells and subsequent immunoprecipitation of DNA cross-linked to acetylated histones
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions1 year at 4°C from date of shipment.
      Packaging Information
      Material Size2.5 mL
      Material Package2.5 mL packed beads provided as a 50% gel slurry for a final volume of 5 mL.
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Protein G Agarose/Salmon Sperm DNA - 2145457 2145457
      Protein G Agarose/Salmon Sperm DNA - 2384727 2384727
      Protein G Agarose/Salmon Sperm DNA - 2397123 2397123
      Protein G Agarose/Salmon Sperm DNA - 0607035976 0607035976
      Protein G Agarose/Salmon Sperm DNA - 0609040080 0609040080
      Protein G Agarose/Salmon Sperm DNA - 0702051787 0702051787
      Protein G Agarose/Salmon Sperm DNA - 1957268 1957268
      Protein G Agarose/Salmon Sperm DNA - 2004863 2004863
      Protein G Agarose/Salmon Sperm DNA - 2035551 2035551
      Protein G Agarose/Salmon Sperm DNA - 2073148 2073148
      Protein G Agarose/Salmon Sperm DNA - 2231339 2231339
      Protein G Agarose/Salmon Sperm DNA - 2299560 2299560
      Protein G Agarose/Salmon Sperm DNA - 2299962 2299962
      Protein G Agarose/Salmon Sperm DNA - 24283 24283
      Protein G Agarose/Salmon Sperm DNA - 26126 26126
      Protein G Agarose/Salmon Sperm DNA - 27943 27943
      Protein G Agarose/Salmon Sperm DNA - 29605 29605
      Protein G Agarose/Salmon Sperm DNA - 31619 31619
      Protein G Agarose/Salmon Sperm DNA - 32606 32606
      Protein G Agarose/Salmon Sperm DNA - 33567 33567
      Protein G Agarose/Salmon Sperm DNA - DAM1408846 DAM1408846
      Protein G Agarose/Salmon Sperm DNA - DAM1557507 DAM1557507
      Protein G Agarose/Salmon Sperm DNA - DAM1767532 DAM1767532
      Protein G Agarose/Salmon Sperm DNA - DAM1807959 DAM1807959
      Protein G Agarose/Salmon Sperm DNA - JBC1370705 JBC1370705
      Protein G Agarose/Salmon Sperm DNA - JBC1865939 JBC1865939
      Protein G Agarose/Salmon Sperm DNA - JBC1920638 JBC1920638
      Protein G Agarose/Salmon Sperm DNA -2465251 2465251
      Protein G Agarose/Salmon Sperm DNA -2604780 2604780
      Protein G Agarose/Salmon Sperm DNA -2727203 2727203

      References

      Reference overviewApplicationPub Med ID
      Functional analysis of epigenetic regulation of tandem RhopH1/clag genes reveals a role in Plasmodium falciparum growth.
      Comeaux, CA; Coleman, BI; Bei, AK; Whitehurst, N; Duraisingh, MT
      Molecular microbiology 80 378-90 2011

      Show Abstract
      Immunoprecipitation21320181 21320181
      Herpes Simplex Virus 1 ICP4 Forms Complexes with TFIID and Mediator in Virus-Infected Cells.
      Lester, Jonathan T and Deluca, Neal A
      J. Virol., 85: 5733-44 (2011) 2011

      Show Abstract
      21450820 21450820
      Interaction between Hhex and SOX13 Modulates Wnt/TCF Activity.
      Marfil V, Moya M, Pierreux CE, Castell JV, Lemaigre FP, Real FX, Bort R
      The Journal of biological chemistry 285 5726-37 2010

      Show Abstract Full Text Article
      20028982 20028982
      MUC1 regulates nuclear localization and function of the epidermal growth factor receptor.
      Bitler BG, Goverdhan A, Schroeder JA
      J Cell Sci 123 1716-23. Epub 2010 Apr 20. 2010

      Show Abstract Full Text Article
      20406885 20406885
      Ikaros is a regulator of Il10 expression in CD4+ T cells.
      Sarah E Umetsu,Susan Winandy
      Journal of immunology (Baltimore, Md. : 1950) 183 2009

      Show Abstract Full Text Article
      19828627 19828627
      Transcriptional regulation of CD4 gene expression by T cell factor-1/beta-catenin pathway.
      Zhaofeng Huang, Huimin Xie, Vassilio Ioannidis, Werner Held, Hans Clevers, Maureen S Sadim, Zuoming Sun
      Journal of immunology (Baltimore, Md. : 1950) 176 4880-7 2006

      Show Abstract
      16585583 16585583