A part of MilliporeSigma

2100 | Protein-Concentrate Kit (Micro)

10 units  10 samples
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      Replacement Information

      Key Specifications Table

      Key Applications
      Protein Concentration
      Catalogue Number2100
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionProtein-Concentrate Kit (Micro)
      OverviewThe Chemicon Protein-Concentrate kit uses a proprietary reagent Universal Protein Precipitation Agent (UPPA). Protein solutions as dilute as 1 ng/mL can be quantitatively concentrated into a small volume. Protein precipitation is not effected by the presence of detergents, chaotropic, or other common laboratory agents. After precipitation, the precipitate is suspended in a small volume of Precipitate Suspension Buffer supplied with each kit. If the protocol is followed correctly, the recovery is generally 100%. There are enough reagents for concentrating up to 10 mL of dilute protein solution.
      Materials Required but Not DeliveredCentrifuge

      Centrifuge tubes


      Spin columns
      Product Information
      • UPPA-I 30 Ml
      • UPPA-II 30 mL
      • OrgoSol Buffer 50 mL
      • UPC-Wash 2.0 mL
      • UPC-SEED 0.3 mL
      • Precipitate Buffer I 2.0 mL
      • Precipitate Buffer II 0.5 mL
      ApplicationProtein-Concentrate Kit (Micro) is suitable for concentrating proteins for running gels, raising antibodies, protein purification, protein assays & other applications.
      Key Applications
      • Protein Concentration
      Application NotesSuitable for concentrating proteins for running gels, raising antibodies, protein purification, protein assays and other applications.

      This kit is NOT suitable for those proteins which may lose some of their biological activities when precipitated.


      ImportantPerform the entire procedure in the cold (ice bucket) unless specified otherwise. Concentration should be performed in a centrifuge tube. For small volumes, use microfuge tubes. Always position microfuge tubes in the centrifuge at the same orientation, i.e. cap-hinge facing out-ward, this will allow the pellet to remain glued to the same side of the tube during repeated centrifugations and minimize the loss of protein pellets.

      1. Mix 1 volume of protein solution to be concentrated with 3 volumes of UPPA-I. Vortex well and incubate at 4-5°C (ice bucket) for 10-15 min.

      2. Add 3 volumes of UPPA-II. Vortex and the tube.Example: For 0.1 mL of protein solution, add 0.3 mL of UPPA-I, incubate and then add 0.3 mL of UPPA-II.

      3. Centrifuge the tube at 15,000xg for 5 minutes to form a tight pellet.

      4. As soon as the centrifuge stops, remove the tube from the centrifuge. (NOTE: Pellets should not be allowed to diffuse after centrifugation is complete.)

      5. Using a pipet tip, carefully and without disturbing the pellet, remove the entire supernatant.

      6. Carefully reposition the tube in the centrifuge as before, i.e. cap-hinge facing out-ward. Centrifuge the tube again for 30 seconds. Use a pipet tip and remove the remaining supernatant.

      7. Add 40 mL of UPC-Wash on top of the pellet. Carefully reposition the tube in the centrifuge as before, i.e. cap-hinge facing out-ward. Centrifuge the tube again for 5 minutes. Using a pipet tip, carefully and without disturbing the pellet, remove the Wash solution.

      8. Add 25 mL of pure water on top of the pellet. Vortex the tube to suspend the pellet. Please note: pellets do not dissolve in water.

      9. Add 1-5 mL of OrgoSol Buffer, pre-chilled at -20°C, and 5 mL of UPC-SEED. NOTE: for each 0.1-0.3 mL of protein solution add 1 mL OrgoSol Buffer. In addition, OrgoSol Buffer must be at least 10 fold in excess of the water added in Step 8.Vortex to suspend the pellet. It is important that the pellet is fully suspended in OrgoSol Buffer. Please note: pellets will not dissolve in OrgoSol Buffer.Incubate the tube at -20°C for 30 minutes. Periodically vortex the tube, 20-30 seconds vortex each time.

      10. Centrifuge at 15,000xg for 5 minutes to form a tight pellet.

      11. Remove and discard the supernatant. You will notice a white pellet in the tube. Air dry the pellet. On drying, the white pellet will turn translucent. NOTE: do not over dry the pellets - parched pellets may be difficult to dissolve.

      12. Suspend the pellet in an appropriate volume of Precipitate Buffer-I (5-50 μL Precipitate Buffer-I). Vortex to suspend the pellet. Incubate for 2 minutes.

      13. Add Precipitate Buffer-II. For each 5 mL Precipitate Buffer-I used, add 1 mL of Precipitate Buffer-II. Incubate for 5 minutes. After the pellet is dissolved, centrifuge and collect a clear protein solution. The protein solution at this stage contains 60 mM Tris, pH 7-7.5.After dissolving the pellet, the protein suspension may be mixed with SDS, Urea, GunHCl, SDS-PAGE gel loading buffer or other types of buffers and agents.For buffer exchange, the protein suspension may be dialyzed or passed through a pre-equilibrated spin column.


      Samples containing > 100 mg protein produce large and tightly packed protein pellets which require a longer time to dissolve in buffers. Grinding of the protein pellet with a pestle will accelerate solubilization of the pellet.
      Biological Information
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at room temperature for up to 6 months from date of purchase. To avoid possible microbial contamination, dispense all solutions aseptically.
      Packaging Information
      Material Size10 units
      Material Package10 samples
      Transport Information
      Supplemental Information


      Certificates of Analysis

      TitleLot Number
      PROTEIN-CONCENTRATE KIT (MICRO) - 1979934 1979934
      PROTEIN-CONCENTRATE KIT (MICRO) - 2000375 2000375
      PROTEIN-CONCENTRATE KIT (MICRO) -2664963 2664963
      PROTEIN-CONCENTRATE KIT (MICRO) -2780592 2780592
      PROTEIN-CONCENTRATE KIT (MICRO) Concentrate Dilute Protein Solutions - 2390386 2390386
      PROTEIN-CONCENTRATE KIT (MICRO) Concentrate Dilute Protein Solutions - 2019755 2019755
      PROTEIN-CONCENTRATE KIT (MICRO) Concentrate Dilute Protein Solutions - 2035051 2035051
      PROTEIN-CONCENTRATE KIT (MICRO) Concentrate Dilute Protein Solutions - 2191936 2191936
      PROTEIN-CONCENTRATE KIT (MICRO) Concentrate Dilute Protein Solutions - 2193291 2193291
      PROTEIN-CONCENTRATE KIT (MICRO) Concentrate Dilute Protein Solutions - 2262653 2262653
      PROTEIN-CONCENTRATE KIT (MICRO) Concentrate Dilute Protein Solutions -2689042 2689042

      References | 24 Available | See All References

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      J Pharmacol Exp Ther 334 634-41. Epub 2010 May 19. 2010

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      Custom-designed MLPA using multiple short synthetic probes: application to methylation analysis of five promoter CpG islands in tumor and urine specimens from patients with bladder cancer.
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      J Mol Diagn 12 402-8. Epub 2010 Apr 22. 2010

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      Sucrose nonfermenting AMPK-related kinase (SNARK) mediates contraction-stimulated glucose transport in mouse skeletal muscle.
      Koh HJ, Toyoda T, Fujii N, Jung MM, Rathod A, Middelbeek RJ, Lessard SJ, Treebak JT, Tsuchihara K, Esumi H, Richter EA, Wojtaszewski JF, Hirshman MF, Goodyear LJ
      Proc Natl Acad Sci U S A 2010

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      Impairment of PGC-1alpha expression, Neuropathology and Hepatic Steatosis in a transgenic mouse model of Huntington's disease following chronic energy deprivation.
      Chaturvedi RK, Calingasan NY, Yang L, Hennessey T, Johri A, Beal MF
      Hum Mol Genet 2010

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      Tbr1 regulates regional and laminar identity of postmitotic neurons in developing neocortex.
      Bedogni F, Hodge RD, Elsen GE, Nelson BR, Daza RA, Beyer RP, Bammler TK, Rubenstein JL, Hevner RF
      Proc Natl Acad Sci U S A 107 13129-34. Epub 2010 Jul 6. 2010

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      Data Sheet

      Protein-Concentrate Kit (Micro) - Data Sheet