A part of MilliporeSigma

539253 | Protein A, Peroxidase Conjugate

539253
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      View Pricing & Availability
      Click To Print This Page

      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      539253-1MG
      Retrieving availability...
      Limited AvailabilityLimited Availability
      Stocked 
      Discontinued
      Limited Quantities Available
      Available
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service

          Glass bottle 1 mg
          Retrieving price...
          Price could not be retrieved
          Minimum Quantity needs to be mulitiple of
          Upon Order Completion More Information
          You Saved ()
           
          Request Pricing
          Description
          OverviewNative protein A conjugated to horseradish peroxidase.
          Catalogue Number539253
          Brand Family Calbiochem®
          References
          Product Information
          FormLiquid
          FormulationIn 10 mM PBS, 0.5 mM EDTA, 10% mannose, 2% sucrose, 1% BSA, pH 7.6.
          Preservative0.02% thimerosal
          Applications
          Key Applications Enzyme-Linked Immunosorbent Assay
          Immunoblotting (Western Blotting)
          Application NotesDirect Immunoassay (1:10,000)
          Application Commentso-Phenylenediamine, 1.0 mg/ml in citrate buffer, pH 4.5, 0.1% urea hydrogen peroxide at 25°C was used as a substrate for testing conjugate enzyme reactivity. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          PuritySingle band 50,000 on SDS-PAGE
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Yes
          Mercury prohibited To comply with ban of sale of mercury-added products required by The Interstate Mercury Education and Reduction Clearinghouse (IMERC), this product is prohibited to be sold in the following US states: Rhode Island and Connecticut.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          539253

          Brochure

          Title
          Enzyme-Linked Detection Systems Technical Bulletin
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision13-August-2007 RFH
          ApplicationDirect Immunoassay (1:10,000)
          DescriptionNative protein A from Staphylococcus aureus conjugated to horseradish peroxidase.
          BackgroundProtein A is the universal secondary antibody because it binds to the Fc portions of most immunoglobulin classes. It is a versatile and economic means to isolate, detect and purify antibodies produced by many animals. It is especially useful in immunohistochemical studies because, unlike secondary antibodies, it does not bind to cell Fc receptors and therefore will not give high background reactions.
          FormLiquid
          FormulationIn 10 mM PBS, 0.5 mM EDTA, 10% mannose, 2% sucrose, 1% BSA, pH 7.6.
          Concentration Label Please refer to vial label for lot-specific concentration
          PuritySingle band 50,000 on SDS-PAGE
          Preservative0.02% thimerosal
          Commentso-Phenylenediamine, 1.0 mg/ml in citrate buffer, pH 4.5, 0.1% urea hydrogen peroxide at 25°C was used as a substrate for testing conjugate enzyme reactivity. Variables associated with assay conditions will dictate the proper working dilution.
          Storage +2°C to +8°C
          Do Not Freeze Yes
          Toxicity Standard Handling