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500502 | Proteasome Inhibitor XXI, Z-LLLL-SF - Calbiochem

500502
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      Overview

      Replacement Information

      Key Specifications Table

      Empirical Formula
      C₃₂H₅₃FN₄O₇S

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      5.00502.0001
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      Stocked 
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          Glass bottle 5 mg
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          Description
          OverviewA cell-permeable tetrapeptidylsulfonylfluoride-based irreversible proteasome inhibitor that selectively inhibits the hydrolysis of Suc-LLVY-AMC (Cat. No. 539142) by the β5 chymotrypsin-like activity (IC50 = 7 nM using yeast 20S proteasome), while exhibiting much reduced potency against Z-LLE-AMC (Cat. No. 539141) hydrolysis by the β1 caspase-like activity or Z-LLR-AMC hydrolysis by the β2 trypsin-like activity (29% and 75% inhibition, respectively, with 200 µM inhibitor). One hour inhibitor treatment at 5 µM in HEK cultures is shown to completely abolish β5 labeling by the proteasome probe MV151, while only partial MV151 blockage is seen with β1 and β2 at an inhibitor concentration of 50 µM.

          Please note that the molecular weight for this compound is batch-specific due to variable water content. Please refer to the vial label or the certificate of analysis for the batch-specific molecular weight. The molecular weight provided represents the baseline molecular weight without water.
          Catalogue Number500502
          Brand Family Calbiochem®
          SynonymsCbz-Leu₄-SF, Cbz-LLLL-SF, Z-Leu₄-SF, Z-L₄-SF, Cbz-Leu-Leu-Leu-Leu-ψ-[CH2SO2]-F
          References
          ReferencesBrouwer. A.J., et al. 2013. J. Med. Chem. 55, 10995.
          Product Information
          FormWhite powder
          Hill FormulaC₃₂H₅₃FN₄O₇S
          Chemical formulaC₃₂H₅₃FN₄O₇S
          ReversibleN
          Structure formula Image
          Applications
          Biological Information
          Primary Targetβ5 chymotrypsin-like activity of 20S proteasome
          Purity≥98% by HPLC
          Physicochemical Information
          Cell permeableY
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Shipped with Blue Ice or with Dry Ice
          Toxicity Standard Handling
          Storage -20°C
          Protect from Light Protect from light
          Protect from Moisture Protect from moisture
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C. Stock solutions are stable for up to 6 months at -20°C.
          Packaging Information
          Packaged under inert gas Packaged under inert gas
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          References

          Reference overview
          Brouwer. A.J., et al. 2013. J. Med. Chem. 55, 10995.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision26-July-2013 JSW
          SynonymsCbz-Leu₄-SF, Cbz-LLLL-SF, Z-Leu₄-SF, Z-L₄-SF, Cbz-Leu-Leu-Leu-Leu-ψ-[CH2SO2]-F
          DescriptionA cell-permeable tetrapeptidylsulfonylfluoride-based irreversible proteasome inhibitor that selectively inhibits the hydrolysis of Suc-LLVY-AMC (Cat. No. 539142) by the β5 chymotrypsin-like activity (IC50 = 7 nM using yeast 20S proteasome; [E]0 = 0.1 nM, [S]0 = 250 µM; 15 min preincubation before substrate addition), while exhibiting much reduced potency against Z-LLE-AMC (Cat. No. 539141) hydrolysis by the β1 caspase-like activity or Z-LLR-AMC hydrolysis by the β2 trypsin-like activity (29% and 75% inhibition, respectively; 15 min preincubation with 200 µM inhibitor). One hour inhibitor treatment at 5 µM in HEK cultures is shown to completely abolish β5 labeling by the proteasome probe MV151, while only partial MV151 blockage is seen with β1 and β2 subunits at an inhibitor concentration of 50 µM.
          FormWhite powder
          Intert gas (Yes/No) Packaged under inert gas
          Chemical formulaC₃₂H₅₃FN₄O₇S
          Structure formula
          Purity≥98% by HPLC
          SolubilityDMSO (100 mg/ml)
          Storage -20°C
          Protect from light
          Protect from moisture
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C. Stock solutions are stable for up to 6 months at -20°C.
          Toxicity Standard Handling
          ReferencesBrouwer. A.J., et al. 2013. J. Med. Chem. 55, 10995.