539125 Protease Assay Kit

539125
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service
      View Pricing & Availability

      Overview

      Replacement Information

      Key Specifications Table

      Detection Methods
      Colorimetric

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      539125-1KIT
      Retrieving availability...
      Limited AvailabilityLimited Availability
      Stocked 
      Discontinued
      Limited Quantities Available
      Available
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service
          Contact Customer Service

          Glass bottle 1 kit
          Retrieving price...
          Price could not be retrieved
          Minimum Quantity needs to be mulitiple of
          Upon Order Completion More Information
          You Saved ()
           
          Request Pricing
          Description
          OverviewA kit suitable for quantitative array of a wide variety of proteases in biological samples, utilizing FTC-casein as a substrate. Enzyme activities that have been detected with this assay kit include: chymotrypsin, elastase, plasminogen, PRONASE® Protease, subtilisin, thermolysin, and trypsin.
          Catalogue Number539125
          Brand Family Calbiochem®
          Materials Required but Not Delivered Trichloroacetic acid (TCA), 5%
          Spectrophotometer that can accurately measure the absorbance at 492 nm
          Pipetting devices for accurate delivery of volumes required for the assay
          Cuvettes with optical properties suitable for use at 492 nm
          A constant-temperature water bath set at 37°C
          Microfuge for generating approximately 12,000 x g
          References
          ReferencesTwining, S.S. 1984. Anal. Biochem. 143, 30.
          Wiesner, R., et al. 1982. Anal. Biochem. 121, 290.
          Product Information
          Detection methodColorimetric
          Form200 Tests
          FormatCuvette or 96-well plate
          Kit containsA Fluorescent-Casein Substrate, Incubation Buffer, Assay Buffer Trypsin Standard, and a user protocol.
          Applications
          Biological Information
          Assay time3 h
          Sample TypeBiological samples
          Physicochemical Information
          Sensitivity10 ng/ml
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Multiple Toxicity Values, refer to MSDS
          Storage -20°C
          Storage Conditions FTC-casein should be stored at -20°C and is stable for one year from the date of purchase. FTC-casein stored at 4°C is stable for 2-3 days. Several freeze-thaw cycles will lead to a minor increase in the background fluorescence, so following initial thawing, we suggest that this reagent be divided into 5 aliquots. FTC-casein is light sensitive and should be protected from light.
          Protease positive control should be stored at -20°C. As with many proteases, trypsin is susceptible to autolysis and you may notice a reduction in trypsin activity with time, even if stored at -20°C.
          Incubation buffer and assay buffer may be stored at 4°C or -20°C.
          Protect from Light Protect from light
          Do not freeze Ok to freeze
          Packaging Information
          Transport Information
          Supplemental Information
          Kit containsA Fluorescent-Casein Substrate, Incubation Buffer, Assay Buffer Trypsin Standard, and a user protocol.
          Specifications

          Documentation

          Protease Assay Kit SDS

          Title

          Safety Data Sheet (SDS) 

          Protease Assay Kit Certificates of Analysis

          TitleLot Number
          539125

          References

          Reference overview
          Twining, S.S. 1984. Anal. Biochem. 143, 30.
          Wiesner, R., et al. 1982. Anal. Biochem. 121, 290.

          User Protocols

          Title
          User Protocol