A-003-E | Poly-D-Lysine solution, 1.0 mg/mL

A-003-E
20 mL  
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      Overview

      Replacement Information

      Key Specifications Table

      Key Applications
      CULT
      Description
      Catalogue NumberA-003-E
      Trade Name
      • Specialty Media
      DescriptionPoly-D-Lysine solution, 1.0 mg/mL
      OverviewPoly-D-Lysine promotes the adhesion of tissues/sections to the culture vessel. The polycationic property of the molecule allows interaction with anionic sites on the tissue/cells promoting effective attachment to the growing surface. The molecular weight of Poly-D-Lysine is >300,000 Da.
      Alternate Names
      • poly lysine
      References
      Product Information
      PresentationLiquid. 20ml in sterile water.
      Applications
      ApplicationThe Poly-D-Lysine solution promotes the adhesion of tissues/sections to the culture vessel.
      Key Applications
      • Cell Culture
      Application NotesThe level of coating will vary for different species and types of cells. In general final coating concentrations from 3-10μg/cm(2) of surface area will typically supply sufficent coatings for most cell types.



      Surface area of plates: (pi)(Radius squared) in cm(2)



      In partical terms working dilutions of 50μg/mL to 100μg/mL are usually adequate.



      Dilute poly-D-lysine in PBS, water, or 0.1M borate buffer {prepared by adding 1.24 g boric acid and 1.9 g sodium tetraborate in 400 ml water, pH 8.5} to final working concentration.

      Plates or flasks are coated for 3 hours to overnight, and up to 3 days if stored at 4C. Best results are usually with just over night coatings.

      Remove poly-d-lysine solution and rinse with sterile PBS or water 1-2X. Do not scrape the bottom of the coated plates or flasks.

      Use plates immediately or store with PBS for up to 5 days.



      Poly-D-lysine coating methods will vary slightly from laboratory to laboratory, please use the method as a general guide.
      Biological Information
      Media FormLiquid
      Stem Cell Type
      • Human Embryonic Stem Cells
      • Mesenchymal Stem Cells
      • Neural Stem Cells
      • Hematopoietic Stem Cells
      • Epithelial Cells
      • Pancreatic Stem Cells
      • Induced Pluripotent Stem Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -20C for upto 18 months.
      Packaging Information
      Material Size20 mL
      Transport Information
      Supplemental Information
      Specifications