346104 | Plk2, GST- Fusion, Human, Recombinant, S. frugiperda

346104
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      Overview

      Replacement Information

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      346104-10UG
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          Plastic ampoule 10 μg
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          Description
          OverviewFull length, recombinant, human Plk2 fused at the N-terminus to GST and expressed in S. frugiperda insect cells using a baculovirus expression system. Plk2 is a G1 specific kinase that is specifically detected in brain, heart, and lung. Plk2 expression increases in the presence of DNA-damaging agents. The active kinase is useful for the study of Plk2 regulation and inhibitor screening.
          Catalogue Number346104
          Brand Family Calbiochem®
          SynonymsPolo-like kinase 2, Human, Recombinant
          Application Data
          Phosphorylation of SER/THR 16 substrate was measured in the presence of increasing concentration of Plk2 as outlined in the Recommended Reaction Conditions above.
          References
          ReferencesEckerdt, F., et al. 2005. Oncogene 24, 267.
          Warnke, S., et al. 2004.Curr Biol. 14, 1200.
          Product Information
          Unit of DefinitionOne unit is defined as the amount of enzyme that will phosphorylate 1 pmol SER/THR 16 substrate per min at 30°C, pH 7.5.
          FormLiquid
          FormulationIn 100 mM NaCl, 25 mM Tris-HCl, 3 mM DTT, 50% glycerol, 0.05% Tween®-20 detergent, pH 8.0.
          Applications
          Biological Information
          Purity≥70% by SDS-PAGE
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          S PhraseS: 26-45

          In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
          In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage ≤ -70°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          346104

          References

          Reference overview
          Eckerdt, F., et al. 2005. Oncogene 24, 267.
          Warnke, S., et al. 2004.Curr Biol. 14, 1200.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision13-August-2008 JSW
          SynonymsPolo-like kinase 2, Human, Recombinant
          Application Data
          Phosphorylation of SER/THR 16 substrate was measured in the presence of increasing concentration of Plk2 as outlined in the Recommended Reaction Conditions above.
          DescriptionFull length, recombinant, human Plk2 fused at the N-terminus to GST and expressed in S. frugiperda insect cells using a baculovirus expression system. Plk2 is a G1 specific kinase that is specifically detected in brain, heart, and lung. Plk2 expression increases in the presence of DNA-damaging agents. The active kinase is useful for the study of Plk2 regulation and inhibitor screening.
          FormLiquid
          FormulationIn 100 mM NaCl, 25 mM Tris-HCl, 3 mM DTT, 50% glycerol, 0.05% Tween®-20 detergent, pH 8.0.
          Concentration Label Please refer to vial label for lot-specific concentration
          Recommended reaction conditions
          50 mM HEPES, 10 mM MgCl2, 1 mM EGTA, 0.01% Brij®-35 detergent, 200 µM ATP, 2 µM SER/THR 16 substrate; incubate at room temperature for 1 h.
          Purity≥70% by SDS-PAGE
          Unit definitionOne unit is defined as the amount of enzyme that will phosphorylate 1 pmol SER/THR 16 substrate per min at 30°C, pH 7.5.
          Storage ≤ -70°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Toxicity Standard Handling
          ReferencesEckerdt, F., et al. 2005. Oncogene 24, 267.
          Warnke, S., et al. 2004.Curr Biol. 14, 1200.