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14-573 | Pim1 Protein, active, 10 µg

14-573
10 µg  Also available in 250 µg size --call for pricing and availability and reference catalog number 14-573M when ordering the 250 µg size.
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      Overview

      Replacement Information
      Description
      Catalogue Number14-573
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionPim1 Protein, active, 10 µg
      OverviewN-terminal GST-tagged recombinant human full length Pim-1
      References
      Product Information
      Applications
      ApplicationActive, N-terminal GST-tagged recombinant human full length Pim-1, for use in Kinase Assays.
      Key Applications
      • Kinase Assay
      Biological Information
      Sourceexpressed in E. coli cells
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Entrez Gene SummaryThe protooncogene PIM1 encodes a protein kinase upregulated in prostate cancer (Dhanasekaran et al., 2001 [PubMed 11518967]).[supplied by OMIM]
      Gene Symbol
      • PIM1
      • PIM
      Protein TargetPim-1
      Purification MethodGlutathione agarose affinity chromatography
      Target Sub-FamilyCAMK
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P11309 # Plays a role in signal transduction in blood cells. Contributes to both cell proliferation and survival and thus provide a selective advantage in tumorigenesis. May affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3.
      COFACTOR: Manganese.
      SIZE: 404 amino acids; 45412 Da
      SUBUNIT: Binds to RP9. Isoform 2 is isolated as a monomer whereas isoform 1 complexes with other proteins. Isoform 1, but not isoform 2, binds BMX.
      SUBCELLULAR LOCATION: Isoform 2: Cytoplasm. Nucleus. & Isoform 1: Cell membrane.
      TISSUE SPECIFICITY: Expressed primarily in cells of the hematopoietic and germline lineages. Isoform 1 and isoform 2 are both expressed in prostate cancer cell lines.
      PTM: Autophosphorylated on both serine/threonine and tyrosine residues.
      SIMILARITY: SwissProt: P11309 ## Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily. & Contains 1 protein kinase domain.
      Molecular Weight62kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by phosphorylation of S6 Kinase/Rsk-2 Substrate Peptide 2
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions6 months at -70°C
      Packaging Information
      Material Size10 µg
      Material PackageAlso available in 250 µg size --call for pricing and availability and reference catalog number 14-573M when ordering the 250 µg size.
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Pim-1, active - 1580045-F 1580045-F
      Pim-1, active - 1580045-G 1580045-G
      Pim-1, active - 1580045-H 1580045-H
      Pim-1, active - 2263618-A 2263618-A

      References

      Reference overviewPub Med ID
      New potent dual inhibitors of CK2 and Pim kinases: discovery and structural insights.
      López-Ramos, Miriam, et al.
      FASEB J., 24: 3171-85 (2010) 2010

      Show Abstract
      20400536 20400536

      FAQ

      QuestionAnswer
      What is the Molar extinction coefficient for Adenosine 5'-[g32P]triphosphate ([g32P]ATP) used in the kinase assay? The Molar extinction coefficient is 15400.

      Related Products & Applications

      Alternative Packsize

      Catalog Number Description  
      14-573M Pim1 Protein, active, 250 µg Show Pricing & Availability

      Product Families

      Categories

      Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases
      Life Science Research > Proteins and Enzymes > Purified Kinases