525189 Phospholipase C-γ-2, His•Tag®, Human, Recombinant, S. frugiperda

525189
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      525189-50UG
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          Plastic ampoule 50 μg
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          Description
          OverviewFull-length, recombinant, human phospholipase C γ-2 fused at the N-terminus to His•Tag® and S•Tag™ sequences and expressed in S. frugiperda insect cells. This preparation is qualified for use as a substrate for protein tyrosine kinases in in vitro assays. Intrinsic enzyme activity has not been tested. Production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes, which are crucial enzymes in transmembrane signaling. PLC-γ-2 is phosphorylated on tyrosine residues following ligand-induced activation of a variety of growth factor receptors and immune system receptors, resulting in increased phospholipase activity. The full-length proteins contains the following domains: C2 domain (aa 1059-1152); PH domain (aa 20-131); PI-PLC X-box catalytic domain (aa 312-456); PI-PLC Y-box catalytic domain (aa 930-1044); two SH2 domains (aa 532-635, aa 646-735); and a SH3 domain (aa 769-829). It has been reported to be phosphorylated at Tyr753 and Tyr759 (Btk, Src, Fyn, Lck), and Tyr1217 and Tyr1245 by Bcr-Abl.
          Catalogue Number525189
          Brand Family Calbiochem®
          SynonymsPhospholipase C-γ-2, Human, Recombinant
          Application Data
          References
          ReferencesRodriguez, R., et al. 2001. J. Biol. Chem. 276, 47982.
          Lennon, G., et al. 1996. Genomics 33, 151.
          Product Information
          FormLiquid
          FormulationIn PBS.
          Applications
          Biological Information
          Purity≥90% by SDS-PAGE
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Irritant
          Storage ≤ -70°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          525189

          References

          Reference overview
          Rodriguez, R., et al. 2001. J. Biol. Chem. 276, 47982.
          Lennon, G., et al. 1996. Genomics 33, 151.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision26-August-2008 RFH
          SynonymsPhospholipase C-γ-2, Human, Recombinant
          Application Data
          DescriptionFull-length, recombinant, human phospholipase C γ-2 fused at the N-terminus to His•Tag® and S•Tag™ sequences and expressed in S. frugiperda insect cells. This preparation is qualified for use as a substrate for protein tyrosine kinases in in vitro assays. Intrinsic enzyme activity has not been tested. Production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes, which are crucial enzymes in transmembrane signaling. PLC-γ-2 is phosphorylated on tyrosine residues following ligand-induced activation of a variety of growth factor receptors and immune system receptors, resulting in increased phospholipase activity. The full-length proteins contains the following domains: C2 domain (aa 1059-1152); PH domain (aa 20-131); PI-PLC X-box catalytic domain (aa 312-456); PI-PLC Y-box catalytic domain (aa 930-1044); two SH2 domains (aa 532-635, aa 646-735); and a SH3 domain (aa 769-829). It has been reported to be phosphorylated at Tyr753 and Tyr759 (Btk, Src, Fyn, Lck), and Tyr1217 and Tyr1245 by Bcr-Abl.
          FormLiquid
          FormulationIn PBS.
          Concentration Label Please refer to vial label for lot-specific concentration
          Purity≥90% by SDS-PAGE
          Storage ≤ -70°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Toxicity Irritant
          ReferencesRodriguez, R., et al. 2001. J. Biol. Chem. 276, 47982.
          Lennon, G., et al. 1996. Genomics 33, 151.