524715 Phosphodiesterase, Type V, cGMP-Specific, Bovine, Recombinant, Spodoptera frugiperda

524715
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      524715-50UL
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      Limited AvailabilityLimited Availability
      Stocked 
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      Available
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          Plastic ampoule 50 ul
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          Description
          OverviewRecombinant, bovine c-GMP-specific phosphodiesterase expressed in S. frugiperda insect cells. Supplied as partially purified, catalytically active, dimeric holoenzyme. A target enzyme for Viagra (sildenafil). Suitable for enzymatic studies and as a standard in Western blots using Anti-Phosphodiesterase V Antibody (Cat. No. 524583).
          Catalogue Number524715
          Brand Family Calbiochem®
          SynonymsPDE V, Phosphodiesterase V, cGMP-binding cGMP-specific PDE
          References
          ReferencesGibson, L.C.D., et al. 2006. Eur. J. Pharmacol. 538, 39.
          Webb, D.J., et al. 1999. Am. J. Cardiol. 83, 21C.
          Turko, I.V., et al. 1998. J. Biol. Chem. 273, 6460.
          Product Information
          Activity≥50,000 units/ml
          Unit of DefinitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 pmol cGMP per min at 25°C, pH 7.2.
          FormLiquid
          FormulationIn sodium phosphate buffer, 20% glycerol, pH 7.2.
          Applications
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage ≤ -70°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          524715

          References

          Reference overview
          Gibson, L.C.D., et al. 2006. Eur. J. Pharmacol. 538, 39.
          Webb, D.J., et al. 1999. Am. J. Cardiol. 83, 21C.
          Turko, I.V., et al. 1998. J. Biol. Chem. 273, 6460.

          Brochure

          Title
          Recombinant Active Phosphodiesterases Flyer
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision12-July-2007 JSW
          SynonymsPDE V, Phosphodiesterase V, cGMP-binding cGMP-specific PDE
          DescriptionRecombinant, bovine c-GMP-specific phosphodiesterase expressed in S. frugiperda insect cells. Supplied as partially purified, catalytically active, dimeric holoenzyme. A target enzyme for Viagra (sildenafil). Suitable for enzymatic studies and as a standard in Western blots using Anti-Phosphodiesterase V Antibody (Cat. No. 524583). For enzymatic studies a dilution of 1:1000 recommended. For use as a immunoblot standard 2 to 3 µl per lane is recommended. In immunoblotting, gives band at ~92 kDa.
          FormLiquid
          FormulationIn sodium phosphate buffer, 20% glycerol, pH 7.2.
          Activity≥50,000 units/ml
          Unit definitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 pmol cGMP per min at 25°C, pH 7.2.
          Storage ≤ -70°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Toxicity Standard Handling
          ReferencesGibson, L.C.D., et al. 2006. Eur. J. Pharmacol. 538, 39.
          Webb, D.J., et al. 1999. Am. J. Cardiol. 83, 21C.
          Turko, I.V., et al. 1998. J. Biol. Chem. 273, 6460.