525290 PhosphoDetect™ Anti-Phosphotyrosine Rabbit pAb

525290
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      A Broad Range Of Species Rb Polyclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      525290-100UG
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          Plastic ampoule 100 μg
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          Description
          OverviewRecognizes a broad spectrum of tyrosine-phosphorylated proteins in crude extracts.
          Catalogue Number525290
          Brand Family Calbiochem®
          References
          Product Information
          FormLiquid
          FormulationIn PBS, pH 7.4.
          Preservative≤0.1% sodium azide
          Applications
          Key Applications Enzyme-Linked Immunosorbent Assay
          Immunoblotting (Western Blotting)
          Immunoprecipitation
          Application NotesELISA (1:5000-1:10,000)
          Immunoblotting (1:1000-1:2000)
          Immunoprecipitation (2-5 µg)
          Application CommentsDo not use milk as a blocking agent with this antibody. Milk contains many phosphotyrosines and will adsorb the antibody. Monospecific for phosphotyrosine. Little or no cross-reactivity with phosphothreonine- or phosphoserine-containing proteins. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          Immunogenpurified phosphotyrosine conjugated to KLH
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • A Broad Range Of Species
          Antibody TypePolyclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          525290
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision03-December-2007 JSW
          ApplicationELISA (1:5000-1:10,000)
          Immunoblotting (1:1000-1:2000)
          Immunoprecipitation (2-5 µg)
          DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes phosphotyrosine-containing proteins.
          BackgroundCellular activation, proliferation and differentiation are signaled by phosphorylation of tyrosine residues. Growth factor receptors and oncogene protein kinases are associated with tyrosine phosphorylation. Anti-phosphotyrosine antibodies are indispensable for the study of cellular tyrosine phosphorylation.
          HostRabbit
          Immunogenpurified phosphotyrosine conjugated to KLH
          IsotypeIgG
          Speciesa broad range of species
          FormLiquid
          FormulationIn PBS, pH 7.4.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsDo not use milk as a blocking agent with this antibody. Milk contains many phosphotyrosines and will adsorb the antibody. Monospecific for phosphotyrosine. Little or no cross-reactivity with phosphothreonine- or phosphoserine-containing proteins. Variables associated with assay conditions will dictate the proper working dilution.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling