ST1029 PhosphoDetect™ Anti-Fos (pSer³⁷⁴) Mouse mAb (34E4)

ST1029
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H M Monoclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      ST1029-1SET
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      Stocked 
      Discontinued
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      Available
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          Plastic ampoule 1 set
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          Description
          OverviewRecognizes the ~55 kDa c-Fos protein phosphorylated at Ser374. Does not detect the unphosphorylated form.
          Catalogue NumberST1029
          Brand Family Calbiochem®
          Application Data
          Detection of human Fos, phosphorylated on Ser374, by immunoblotting. Samples: Serum starved HepG2 cells incubated with EGF for 0 min (lane 1), 5 min (lane 2), 15 min (lane 3), 30 min (lane 4), 1 h (lane 5), 2 h (lane 6), 4 h (lane 7), and 8 h (lane 8). Primary antibody: PhosphoDetect™ Anti-Fos (pSer374) Mouse mAb (34E4) (Cat. No. ST1029) (0.5 µg/ml). Detection: chemiluminescence.
          References
          ReferencesChen, R-H., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 10952.
          Product Information
          FormLyophilized
          Formulation100 µg antibody lyophilized from 2X PBS, PEG, sucrose and 200 µl lyophilized control lysate from EGF-treated HEPG2 cells.
          Positive controlHepG2 cells treated with EGF
          Preservative≤0.1% sodium azide (antibody only)
          Applications
          Key Applications Enzyme-Linked Immunosorbent Assay
          Immunoblotting (Western Blotting)
          Application NotesELISA (0.1 µg/ml)
          Immunoblotting (0.5 µg/ml, chemiluminescence)
          Application CommentsDoes not detect the unphosphorylated form of c-fos. For immunoblotting use 20 µl control lysate per lane (minigel) for chemiluminescent detection. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser³⁷⁴ phosphorylation site of human c-Fos
          ImmunogenHuman
          Clone34E4
          HostMouse
          IsotypeIgG₁
          Species Reactivity
          • Human
          Antibody TypeMonoclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Shipped with Blue Ice or with Dry Ice
          Toxicity Irritant
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsReconstitute antibody with 1 ml water (15 minutes, room temperature). Following reconstitution, aliquot and freeze in liquid nitrogen. Reconstituted antibody can be stored at -80°C for up to 1 year. Aliquots may be stored at 4°C for up to 3 months and should be thawed at 37°C. Reconstitute control lysate with 200 µl H₂O. After complete solubilization of the proteins add 200 µl SDS-PAGE sample buffer and incubate at 90°C for 5 min. Following reconsitution aliquot and freeze (-20°C). Avoid freeze/thaw cycles.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          PhosphoDetect™ Anti-Fos (pSer³⁷⁴) Mouse mAb (34E4) SDS

          Title

          Safety Data Sheet (SDS) 

          PhosphoDetect™ Anti-Fos (pSer³⁷⁴) Mouse mAb (34E4) Certificates of Analysis

          TitleLot Number
          ST1029

          References

          Reference overview
          Chen, R-H., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 10952.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision17-October-2007 RFH
          ApplicationELISA (0.1 µg/ml)
          Immunoblotting (0.5 µg/ml, chemiluminescence)
          Application Data
          Detection of human Fos, phosphorylated on Ser374, by immunoblotting. Samples: Serum starved HepG2 cells incubated with EGF for 0 min (lane 1), 5 min (lane 2), 15 min (lane 3), 30 min (lane 4), 1 h (lane 5), 2 h (lane 6), 4 h (lane 7), and 8 h (lane 8). Primary antibody: PhosphoDetect™ Anti-Fos (pSer374) Mouse mAb (34E4) (Cat. No. ST1029) (0.5 µg/ml). Detection: chemiluminescence.
          DescriptionMouse monoclonal antibody purified from serum-free cell culture supernatant by thiophilic adsorption and size exclusion chromatography. Supplied with a positive control lysate consisting of EGF-treated HepG2 cells. Recognizes the ~55 kDa c-fos protein phosphorylated at Ser374 by MAP kinase.
          BackgroundThe early gene product c-Fos is expressed following mitogenic stimulation and functions as a sensor for MAPK signal duration. When MAPK activation is transient, MAPK activity declines before accumulation of the c-Fos protein. When MAPK activation is sustained, c-Fos is phosphorylated by MAPK at Ser374. Phosphorylation stabilizes the Fos protein and primes c-Fos for additional phosphorylation at Thr325.
          HostMouse
          Immunogen speciesHuman
          Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser³⁷⁴ phosphorylation site of human c-Fos
          Clone34E4
          IsotypeIgG₁
          Specieshuman
          Positive controlHepG2 cells treated with EGF
          FormLyophilized
          Formulation100 µg antibody lyophilized from 2X PBS, PEG, sucrose and 200 µl lyophilized control lysate from EGF-treated HEPG2 cells.
          Preservative≤0.1% sodium azide (antibody only)
          CommentsDoes not detect the unphosphorylated form of c-fos. For immunoblotting use 20 µl control lysate per lane (minigel) for chemiluminescent detection. Antibody should be titrated for optimal results in individual systems.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsReconstitute antibody with 1 ml water (15 minutes, room temperature). Following reconstitution, aliquot and freeze in liquid nitrogen. Reconstituted antibody can be stored at -80°C for up to 1 year. Aliquots may be stored at 4°C for up to 3 months and should be thawed at 37°C. Reconstitute control lysate with 200 µl H₂O. After complete solubilization of the proteins add 200 µl SDS-PAGE sample buffer and incubate at 90°C for 5 min. Following reconsitution aliquot and freeze (-20°C). Avoid freeze/thaw cycles.
          Toxicity Irritant
          ReferencesChen, R-H., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 10952.

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