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676604 | PhosphoDetect™ Anti-VASP (pSer¹⁵⁷) Mouse mAb (5C6)

676604
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H, M M Monoclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      676604-100UG
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      Available
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          Glass bottle 100 μg
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          Description
          OverviewRecognizes the the ~50 kDa VASP protein phosphorylated at Ser157.
          Catalogue Number676604
          Brand Family Calbiochem®
          SynonymsAnti-Vasodilator-stimulated Phosphoprotein, Activated
          Application Data
          Detection of human VASP, phosphorylated on Ser157, by immunoblotting. Samples: Whole cell extracts from human platelets cells left untreated (lane 1) or SNP treated cells (lane 2). Primary antibody: PhosphoDetect™ Anti-VASP (pSer157) Mouse mAb (5C6) (Cat. No. 676604) (0.5 µg/ml). Detection: chemiluminescence.
          References
          ReferencesBear, J.E., et al. 2001. Curr. Opin. Cell. Biol. 13, 158.
          Lawrence, D.W., and Pryzwansky, K.B. 2001. J. Immunol. 166, 5550.
          Burkhardt, M., et al. 2000. J. Biol. Chem. 275, 33536.
          Harbeck, B., et al. 2000. J. Biol. Chem. 275, 40.
          Smolenski, A., et al. 2000. J. Biol. Chem. 275, 25723.
          Product Information
          FormLyophilized
          FormulationLyophilized from PBS, PEG, sucrose.
          Positive controlVanadate-treated A431 cells
          Preservative≤0.1% sodium azide
          Applications
          Key Applications Enzyme-Linked Immunosorbent Assay
          Immunoblotting (Western Blotting)
          Immunocytochemistry
          Immunoprecipitation
          Application NotesELISA (0.05 µg/ml)
          Immunoblotting (1 µg/ml, colorimetric; 0.1 µg/ml, chemiluminescence)
          Immunocytochemistry (1-10 µg/ml)
          Immunoprecipitation (1-10 µg/10⁶ vanadate-treated A431 cells)
          Application CommentsDoes not cross-react with non-phosphorylated VASP or with unrelated phosphorylation sites. For immunocytochemistry, fixation protocol may include up to 0.5% formaldehyde. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser¹⁵⁷ phosphorylation site of human VASP
          ImmunogenHuman
          Clone5C6
          HostMouse
          IsotypeIgG₁
          Species Reactivity
          • Human
          • Mouse
          Antibody TypeMonoclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage -20°C
          Do not freeze Ok to freeze
          Special InstructionsReconstitute in 1 ml sterile, dH₂O, 15 min, room temperature. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot, flash-freeze in liquid nitrogen, and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Avoid freeze/thaw cycles of solutions.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          676604

          References

          Reference overview
          Bear, J.E., et al. 2001. Curr. Opin. Cell. Biol. 13, 158.
          Lawrence, D.W., and Pryzwansky, K.B. 2001. J. Immunol. 166, 5550.
          Burkhardt, M., et al. 2000. J. Biol. Chem. 275, 33536.
          Harbeck, B., et al. 2000. J. Biol. Chem. 275, 40.
          Smolenski, A., et al. 2000. J. Biol. Chem. 275, 25723.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision15-September-2009 JSW
          SynonymsAnti-Vasodilator-stimulated Phosphoprotein, Activated
          ApplicationELISA (0.05 µg/ml)
          Immunoblotting (1 µg/ml, colorimetric; 0.1 µg/ml, chemiluminescence)
          Immunocytochemistry (1-10 µg/ml)
          Immunoprecipitation (1-10 µg/10⁶ vanadate-treated A431 cells)
          Application Data
          Detection of human VASP, phosphorylated on Ser157, by immunoblotting. Samples: Whole cell extracts from human platelets cells left untreated (lane 1) or SNP treated cells (lane 2). Primary antibody: PhosphoDetect™ Anti-VASP (pSer157) Mouse mAb (5C6) (Cat. No. 676604) (0.5 µg/ml). Detection: chemiluminescence.
          DescriptionMouse monoclonal antibody purified from serum-free cell culture supernatant by thiophilic adsorption and size exclusion chromatography. Recognizes the the ~50 kDa VASP protein phosphorylated at Ser157.
          BackgroundVASP (vasodilator-stimulated phosphoprotein), an established substrate of cAMP- and cGMP-dependent protein kinases in vitro and in living cells, is associated with focal adhesions, microfilaments, and membrane regions of high dynamic activity. VASP binds in vitro to profilin, zyxin, vinculin, and the Listeria sp. surface protein ActA. VASP also promotes actin nucleation and binds to actin filaments in vitro and associates with stress fibers in cells. Studies imply that VASP phosphorylation may act as an immediate negative regulator of actin dynamics. Phosphorylation of Ser157 in VASP can be used as a measure of PKA activity.
          HostMouse
          Immunogen speciesHuman
          Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser¹⁵⁷ phosphorylation site of human VASP
          Clone5C6
          IsotypeIgG₁
          Specieshuman, mouse
          Positive controlVanadate-treated A431 cells
          FormLyophilized
          FormulationLyophilized from PBS, PEG, sucrose.
          Preservative≤0.1% sodium azide
          CommentsDoes not cross-react with non-phosphorylated VASP or with unrelated phosphorylation sites. For immunocytochemistry, fixation protocol may include up to 0.5% formaldehyde. Variables associated with assay conditions will dictate the proper working dilution.
          Storage -20°C
          Do Not Freeze Ok to freeze
          Special InstructionsReconstitute in 1 ml sterile, dH₂O, 15 min, room temperature. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot, flash-freeze in liquid nitrogen, and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Avoid freeze/thaw cycles of solutions.
          Toxicity Standard Handling
          ReferencesBear, J.E., et al. 2001. Curr. Opin. Cell. Biol. 13, 158.
          Lawrence, D.W., and Pryzwansky, K.B. 2001. J. Immunol. 166, 5550.
          Burkhardt, M., et al. 2000. J. Biol. Chem. 275, 33536.
          Harbeck, B., et al. 2000. J. Biol. Chem. 275, 40.
          Smolenski, A., et al. 2000. J. Biol. Chem. 275, 25723.

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