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PK1008 | PhosphoDetect™ Anti-PDGFRβ(pTyr⁷⁵¹) Rabbit pAb

PK1008
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H, M, R Rb Polyclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      PK1008-50UL
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      Stocked 
      Discontinued
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      Available
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          Glass bottle 50 ul
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          Description
          OverviewRecognizes the ~190 kDa PDGF receptor-β protein phosphorylated at Tyr751 in PDGF-stimulated L6, HepG2, and NIH3T3 cells.
          Catalogue NumberPK1008
          Brand Family Calbiochem®
          SynonymsAnti-Platelet Derived Growth Factor Receptor-β, Phospho-Specific (Tyr⁷⁵¹)
          Application Data
          Detection of mouse and human PDGFR-β, phosphorylated on Tyr751, by immunoblotting. Samples: Whole cell lysate from L6, HepG2, and NIH3T3 cells left untreated (-) or treated with PDGF (40 ng/ml for 5 min; +). Primary antibody: PhosphoDetect™ Anti-PDGFR-β (pTyr751) Rabbit pAb (Cat. No. PK1007) (1:1000). Detection: chemiluminescence.

          Detection of human PDGFR-β, phosphorylated on Tyr751, by staining paraffin sections. Sample: Breast carcinoma tissue. Primary antibody: PhosphoDetect™ Anti-PDGFR-β (pTyr751) Rabbit pAb (Cat. No. PK1008) (1:50). Detection: DAB with hematoxylin counterstain.

          Detection of mouse PDGFR-β, phosphorylated on Tyr751, by immunoblotting. Sample: NIH3T3 cells left untreated (blue) or treated with PDGF (green). Primary antibodies: PhosphoDetect™ Anti-PDGFR-β (pTyr751) Rabbit pAb (Cat. No. PK1008) (1:200) and irrelevant control antibody (red). Detection: fluorescence.
          References
          ReferencesBetsholtz, C., et al. 2001. Bioessays 23, 494.
          Ostman, A. and Heldin, C.H. 2001. Adv. Cancer Res. 80, 1.
          Ramalingam, K., et al. 1995. Bioorg. Med. Chem. 3, 1263.
          Panyotou, G. et al. 1992. EMBO J. 11, 4261.
          Coughlin, S.R., et al. 1988. Prog. Clin. Biol. Res. 266, 39.
          Deuel, T.F., et al. 1988. Biofactors 1, 213.
          Product Information
          FormLiquid
          FormulationIn 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.
          Positive controlBreast carcinoma and PDGF-treated L6, HepG2, and NIH3T3 cells
          PreservativeNone
          Applications
          Key Applications Flow Cytometry
          Immunoblotting (Western Blotting)
          Immunoprecipitation
          Paraffin Sections
          Application NotesFlow Cytometry (1:200)
          Immunoblotting (1:1000)
          Immunoprecipitation (1:50)
          Paraffin Sections (1:50, heat pre-treatment required; see comments)
          Application CommentsFor staining paraffin sections bring slides to a boil in 1 mM EDTA, pH 8.0 and continue to heat at sub-boiling temperature for 15 min; no cooling is necessary. Wash paraffin sections with 1X TBS, 0.1% Tween®-20 detergent. For immunoblotting membranes should be incubated with antibody diluted in 1X TBS, 5% BSA, 0.1% Tween®-20 detergent at 4°C overnight with gentle shaking. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Tyr⁷⁵¹ phosphorylation site of human PDGF receptor-β, coupled to KLH
          ImmunogenHuman
          HostRabbit
          IsotypeIgG1
          Species Reactivity
          • Human
          • Mouse
          • Rat
          Antibody TypePolyclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Do not freeze Ok to freeze
          Special InstructionsDo not aliquot.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          PK1008

          References

          Reference overview
          Betsholtz, C., et al. 2001. Bioessays 23, 494.
          Ostman, A. and Heldin, C.H. 2001. Adv. Cancer Res. 80, 1.
          Ramalingam, K., et al. 1995. Bioorg. Med. Chem. 3, 1263.
          Panyotou, G. et al. 1992. EMBO J. 11, 4261.
          Coughlin, S.R., et al. 1988. Prog. Clin. Biol. Res. 266, 39.
          Deuel, T.F., et al. 1988. Biofactors 1, 213.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision16-October-2007 RFH
          SynonymsAnti-Platelet Derived Growth Factor Receptor-β, Phospho-Specific (Tyr⁷⁵¹)
          ApplicationFlow Cytometry (1:200)
          Immunoblotting (1:1000)
          Immunoprecipitation (1:50)
          Paraffin Sections (1:50, heat pre-treatment required; see comments)
          Application Data
          Detection of mouse and human PDGFR-β, phosphorylated on Tyr751, by immunoblotting. Samples: Whole cell lysate from L6, HepG2, and NIH3T3 cells left untreated (-) or treated with PDGF (40 ng/ml for 5 min; +). Primary antibody: PhosphoDetect™ Anti-PDGFR-β (pTyr751) Rabbit pAb (Cat. No. PK1007) (1:1000). Detection: chemiluminescence.

          Detection of human PDGFR-β, phosphorylated on Tyr751, by staining paraffin sections. Sample: Breast carcinoma tissue. Primary antibody: PhosphoDetect™ Anti-PDGFR-β (pTyr751) Rabbit pAb (Cat. No. PK1008) (1:50). Detection: DAB with hematoxylin counterstain.

          Detection of mouse PDGFR-β, phosphorylated on Tyr751, by immunoblotting. Sample: NIH3T3 cells left untreated (blue) or treated with PDGF (green). Primary antibodies: PhosphoDetect™ Anti-PDGFR-β (pTyr751) Rabbit pAb (Cat. No. PK1008) (1:200) and irrelevant control antibody (red). Detection: fluorescence.
          DescriptionRabbit polyclonal antibody purified by protein A and immunoaffinity chromatography. Recognizes the ~190 kDa PDGF receptor-β protein phosphorylated at Tyr751.
          BackgroundThe PDGF family of growth factors consists of several disulfide-linked dimeric isoforms-PDGF-AA, PDGF-AB, PDGF-BB, PDGF-CC, and PDGF-DD-that bind in distinct patterns to the specific receptor tyrosine kinases, PDGFR-α and PDGFR-β. PDGFR-α can bind to all isoforms of PDGF except PDGF-DD and PDGFR-β only binds to PDGF-DD. Both receptors can form homo- and heterodimers, including heterodimer formation with EGFR, which can also be stimulated by PDGF. Similar to other receptor tyrosine kinases, ligand binding leads to receptor dimerization and autophosphorylation, which permits binding and activatino of cytoplasmic SH2 domain-containing signal transduction molecules, including Grb2, Src, GAP, PI-3K, PLCγ, and Nck. As a result, a number of different signaling pathways are initiated leading to cell growth, actin reorganization, cell migration, and cell differentiation. Tyr751 of PDGFR-β is the docking site for PI-3K. Phosphorylated pentapeptides derived from Tyr751 are reported to inhibit the association of PI-3K with PDGFR-β.
          HostRabbit
          Immunogen speciesHuman
          Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Tyr⁷⁵¹ phosphorylation site of human PDGF receptor-β, coupled to KLH
          IsotypeIgG1
          Specieshuman, mouse, rat
          Positive controlBreast carcinoma and PDGF-treated L6, HepG2, and NIH3T3 cells
          FormLiquid
          FormulationIn 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.
          PreservativeNone
          CommentsFor staining paraffin sections bring slides to a boil in 1 mM EDTA, pH 8.0 and continue to heat at sub-boiling temperature for 15 min; no cooling is necessary. Wash paraffin sections with 1X TBS, 0.1% Tween®-20 detergent. For immunoblotting membranes should be incubated with antibody diluted in 1X TBS, 5% BSA, 0.1% Tween®-20 detergent at 4°C overnight with gentle shaking. Antibody should be titrated for optimal results in individual systems.
          Storage -20°C
          Do Not Freeze Ok to freeze
          Special InstructionsDo not aliquot.
          Toxicity Standard Handling
          ReferencesBetsholtz, C., et al. 2001. Bioessays 23, 494.
          Ostman, A. and Heldin, C.H. 2001. Adv. Cancer Res. 80, 1.
          Ramalingam, K., et al. 1995. Bioorg. Med. Chem. 3, 1263.
          Panyotou, G. et al. 1992. EMBO J. 11, 4261.
          Coughlin, S.R., et al. 1988. Prog. Clin. Biol. Res. 266, 39.
          Deuel, T.F., et al. 1988. Biofactors 1, 213.