A part of MilliporeSigma

407707 | PhosphoDetect™ Anti-Insulin Receptor (pTyr1162/1163) Rabbit pAb

407707
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      View Pricing & Availability
      Click To Print This Page

      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H Rb Polyclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      407707-10T
      Retrieving availability...
      Limited AvailabilityLimited Availability
      Stocked 
      Discontinued
      Limited Quantities Available
      Available
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service

          Glass bottle 10 t
          Retrieving price...
          Price could not be retrieved
          Minimum Quantity needs to be mulitiple of
          Upon Order Completion More Information
          You Saved ()
           
          Request Pricing
          Description
          OverviewRecognizes the ~95 kDa β subunit of the insulin receptor protein phosphorylated at Tyr1162 and Tyr1163 in insulin receptor transfected cells. Cross-reacts with the insulin-like growth factor-1 receptor phosphorylated at Tyr1135 and Tyr1136.
          Note: 1 T = 1 test. Sufficient for 10 Western mini blots.
          Catalogue Number407707
          Brand Family Calbiochem®
          Application Data
          Detection of insulin receptor, phosphorylated on Tyr 1162/1163, by immunoblotting. Samples: Whole cell lysate from CHO-T cells transfected with an insulin receptor containing vector and left unstimulated (lane 1), or stimulated with insulin (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-Insulin Receptor (pTyr1162/1163) Rabbit pAb (Cat. No. 407707) (1:1000) pre-incubated without peptide (lanes 1, 5), with phosphopeptide immunogen (lane 2), non-phosphorylated peptide corresponding to the phosphopeptide (lane 3), or a generic phosphotyrosine-containing peptide (lane 4). Detection: chemiluminescence.
          References
          ReferencesBevan, P. 2001. J. Cell Sci. 114, 1429.
          Ottensmeyer, F.P., et al. 2000. Biochemistry 39, 12103.
          Virkamaki, A., et al. 1999. J. Clin. Invest. 103, 931.
          Schmid, E., at al. 1998. FASEB J. 12, 863.
          Hari, J. and Roth, R.A. 1987. J. Biol. Chem. 262, 15341.
          Morgan, D.O., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 328.
          White, M.F. and Kahn, C.R. 1986. The Enzymes 17, 247.
          Ebina, Y., et al. 1985. Cell 40, 747.
          Ganguly, S., et al. 1985. Current Topics in Cellular Regulation 27, 83.
          Grunberger, G., et al. 1984. Science 223, 932.
          Product Information
          FormLiquid
          FormulationIn Dulbecco's PBS (without Mg2+ and Ca2+), 50% glycerol, 1 mg/ml BSA, pH 7.3.
          Preservative≤0.1% sodium azide
          Applications
          Key Applications Enzyme-Linked Immunosorbent Assay
          Immunoblotting (Western Blotting)
          Immunocytochemistry
          Application NotesELISA (see comments)
          Immunoblotting (1:1000)
          Immunocytochemistry (see comments)
          Application CommentsThe immunogen sequence is 100% conserved in the mouse and rat insulin recptor protein, but cross-reactivity has not been tested. This antibody has also been reported to work for ELISA and immunocytochemistry. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Tyr1162/1163 phosphorylation sites of human insulin receptor
          ImmunogenHuman
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Human
          Antibody TypePolyclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage ≤ -70°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          407707

          References

          Reference overview
          Bevan, P. 2001. J. Cell Sci. 114, 1429.
          Ottensmeyer, F.P., et al. 2000. Biochemistry 39, 12103.
          Virkamaki, A., et al. 1999. J. Clin. Invest. 103, 931.
          Schmid, E., at al. 1998. FASEB J. 12, 863.
          Hari, J. and Roth, R.A. 1987. J. Biol. Chem. 262, 15341.
          Morgan, D.O., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 328.
          White, M.F. and Kahn, C.R. 1986. The Enzymes 17, 247.
          Ebina, Y., et al. 1985. Cell 40, 747.
          Ganguly, S., et al. 1985. Current Topics in Cellular Regulation 27, 83.
          Grunberger, G., et al. 1984. Science 223, 932.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision14-December-2007 JSW
          ApplicationELISA (see comments)
          Immunoblotting (1:1000)
          Immunocytochemistry (see comments)
          Application Data
          Detection of insulin receptor, phosphorylated on Tyr 1162/1163, by immunoblotting. Samples: Whole cell lysate from CHO-T cells transfected with an insulin receptor containing vector and left unstimulated (lane 1), or stimulated with insulin (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-Insulin Receptor (pTyr1162/1163) Rabbit pAb (Cat. No. 407707) (1:1000) pre-incubated without peptide (lanes 1, 5), with phosphopeptide immunogen (lane 2), non-phosphorylated peptide corresponding to the phosphopeptide (lane 3), or a generic phosphotyrosine-containing peptide (lane 4). Detection: chemiluminescence.
          DescriptionRabbit polyclonal antibody adsorbed with non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the insulin receptor (IR) protein phosphorylated at Tyr1162/1163 and insulin-like growth factor 1 receptor (IGF-1R) protein phosphorylated at Tyr1162/1163.
          BackgroundInsulin initiates its effects on cells through interaction with high-affinity cell surface glycoprotein receptors consisting of two α (~135 kDa) and two β (~95 kDa) subunits linked by disulfide bonds. The α subunits bind insulin and the β subunits become phosphorylated in response to insulin. Insulin-dependent phosphorylation occurs at multiple sites near the carboxyl terminus and in the kinase domain. The receptor protein tyrosine kinase has been implicated as the mediator of most, if not all, of the effects of insulin. Mutations that diminish both the phosphorylation and the internalization of the insulin receptor block insulin action, as do antibodies which inhibit protein tyrosine kinase activity. Cells from patients with diabetic syndromes, characterized by non-responsiveness to insulin, exhibit reduced insulin dependent protein tyrosine kinase activity. Phosphorylation at Tyr 1162, Tyr 1163, and Tyr 1158 is required for the tyrosine kinase activity of the insulin receptor.
          HostRabbit
          Immunogen speciesHuman
          Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Tyr1162/1163 phosphorylation sites of human insulin receptor
          IsotypeIgG
          Specieshuman
          FormLiquid
          FormulationIn Dulbecco's PBS (without Mg2+ and Ca2+), 50% glycerol, 1 mg/ml BSA, pH 7.3.
          Preservative≤0.1% sodium azide
          CommentsThe immunogen sequence is 100% conserved in the mouse and rat insulin recptor protein, but cross-reactivity has not been tested. This antibody has also been reported to work for ELISA and immunocytochemistry. Variables associated with assay conditions will dictate the proper working dilution.
          Storage ≤ -70°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesBevan, P. 2001. J. Cell Sci. 114, 1429.
          Ottensmeyer, F.P., et al. 2000. Biochemistry 39, 12103.
          Virkamaki, A., et al. 1999. J. Clin. Invest. 103, 931.
          Schmid, E., at al. 1998. FASEB J. 12, 863.
          Hari, J. and Roth, R.A. 1987. J. Biol. Chem. 262, 15341.
          Morgan, D.O., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 328.
          White, M.F. and Kahn, C.R. 1986. The Enzymes 17, 247.
          Ebina, Y., et al. 1985. Cell 40, 747.
          Ganguly, S., et al. 1985. Current Topics in Cellular Regulation 27, 83.
          Grunberger, G., et al. 1984. Science 223, 932.