516561 Pertussis Toxin, Bordetella pertussis, Glycerol Solution - CAS 70323-44-3 - Calbiochem

516561
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      Overview

      Replacement Information

      Key Specifications Table

      CAS #
      70323-44-3

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      516561-50UG
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          Alu drum 50 μg
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          Description
          OverviewA protein endotoxin that catalyzes ADP-ribosylation of guanine nucleotide-binding regulatory proteins. The holotoxin has five dissimilar subunits S-1, S-2, S-3, S-4, and S-5, in a molar ratio of 1:1:1:2:1, S-1 (A protomer) is responsible for the enzymatic activity of the toxin, and S-2, S-3, S-4, and S-5 form the B oligomer which is responsible for the toxins binding to the cell surface. NOTE: This product is not activated, hence for use in cell-free systems, activation is required.
          Catalogue Number516561
          Brand Family Calbiochem®
          SynonymsIslet-activating Protein
          References
          ReferencesFryer, M.W. 1992. Neurosci. Lett. 146, 84.
          Gierschik, P. 1992. Curr. Topics Microbiol. Immunol. 175, 69.
          Harada, J., et al. 1992. Eur. J. Pharmacol. 227, 301.
          Hausman, S.Z., and Burns, D.L. 1992. J. Biol. Chem. 267, 13735.
          Kaslow, H.R., and Burns, D.L. 1992. FASEB J. 6, 2684.
          Zhu, J., et al. 1992. J. Pharmacol. Exp. Therap. 263, 1479
          Kaslow, H.R., et al. 1987. Biochemistry 26, 123.
          Hewlett, E.L., et al. 1983. Infect. Immun. 40, 1198.
          Moss, J., et al. 1983. J. Biol. Chem. 258, 11879.
          Product Information
          CAS number70323-44-3
          Activity≤35 pmol/min/µg in the presence of 1 µM calmodulin
          ATP CompetitiveN
          FormLiquid
          FormulationIn 500 mM NaCl, 50 mM Tris, 10 mM glycine, 50% glycerol, pH 7.5.
          ReversibleN
          Applications
          Biological Information
          Biological activity≤1 ng/ml as determined in a clustering assay using CHO cells line
          Primary TargetGuanine nucleotide-binding regulatory proteins
          Primary Target IC<sub>50</sub>≤1 ng/ml as determined in a clustering assay using CHO cells line
          PurityFive distinct bands by SDS-PAGE
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Cell permeableN
          ContaminantsAdenylate cyclase.
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          RTECSXW5883750
          Safety Information
          R PhraseR: 23/24/25

          Toxic by inhalation, in contact with skin and if swallowed.
          S PhraseS: 36/37/39-45

          Wear suitable protective clothing, gloves and eye/face protection.
          In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Toxic
          Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
          Storage -20°C
          Do not freeze Ok to freeze
          Special InstructionsA uniform suspension may be achieved by gentle mixing prior to use.
          Canadian export regulations Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          516561

          References

          Reference overview
          Fryer, M.W. 1992. Neurosci. Lett. 146, 84.
          Gierschik, P. 1992. Curr. Topics Microbiol. Immunol. 175, 69.
          Harada, J., et al. 1992. Eur. J. Pharmacol. 227, 301.
          Hausman, S.Z., and Burns, D.L. 1992. J. Biol. Chem. 267, 13735.
          Kaslow, H.R., and Burns, D.L. 1992. FASEB J. 6, 2684.
          Zhu, J., et al. 1992. J. Pharmacol. Exp. Therap. 263, 1479
          Kaslow, H.R., et al. 1987. Biochemistry 26, 123.
          Hewlett, E.L., et al. 1983. Infect. Immun. 40, 1198.
          Moss, J., et al. 1983. J. Biol. Chem. 258, 11879.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision19-August-2008 RFH
          SynonymsIslet-activating Protein
          DescriptionA protein endotoxin that catalyzes ADP-ribosylation of guanine nucleotide-binding regulatory proteins. Used in the study of adenylate cyclase regulation and the role of Gi proteins. The holotoxin has five dissimilar subunits S-1, S-2, S-3, S-4, and S-5, in a molar ratio of 1:1:1:2:1. S-1 (a protomer) is responsible for the enzymatic activity of the toxin, and the other subunits (2-5) form the B oligomer which is responsible for the toxin's binding to the cell surface. Note: This product is not activated. If used with an intact cell system, the cells will activate the toxin. If used in a cell-free system, product may be activated by the methods of either Kaslow and Burns or Moss at al.(see references).
          FormLiquid
          FormulationIn 500 mM NaCl, 50 mM Tris, 10 mM glycine, 50% glycerol, pH 7.5.
          Concentration Label Please refer to vial label for lot-specific concentration
          CAS number70323-44-3
          RTECSXW5883750
          PurityFive distinct bands by SDS-PAGE
          ContaminantsAdenylate cyclase.
          Activity≤35 pmol/min/µg in the presence of 1 µM calmodulin
          Biological activity≤1 ng/ml as determined in a clustering assay using CHO cells line
          Storage -20°C
          Do Not Freeze Ok to freeze
          Special InstructionsA uniform suspension may be achieved by gentle mixing prior to use.
          Toxicity Toxic
          ReferencesFryer, M.W. 1992. Neurosci. Lett. 146, 84.
          Gierschik, P. 1992. Curr. Topics Microbiol. Immunol. 175, 69.
          Harada, J., et al. 1992. Eur. J. Pharmacol. 227, 301.
          Hausman, S.Z., and Burns, D.L. 1992. J. Biol. Chem. 267, 13735.
          Kaslow, H.R., and Burns, D.L. 1992. FASEB J. 6, 2684.
          Zhu, J., et al. 1992. J. Pharmacol. Exp. Therap. 263, 1479
          Kaslow, H.R., et al. 1987. Biochemistry 26, 123.
          Hewlett, E.L., et al. 1983. Infect. Immun. 40, 1198.
          Moss, J., et al. 1983. J. Biol. Chem. 258, 11879.

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          Categories

          Life Science Research > Inhibitors and Biochemicals > Biochemicals > Toxins