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69149 | Perfect Protein™ Markers, 15-150 kDa

69149
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      69149-0.5ML
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          Glass bottle 0.5 ml
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          69149-3
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              Glass bottle 100 lane
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              69149-EA
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                  Description
                  OverviewThe Perfect Protein™ Markers are a novel set of recombinant proteins with defined sizes at convenient intervals. Designed for routine use in SDS-polyacrylamide gel electrophoresis, the Perfect Protein Markers enable highly accurate size determination of unknown samples. Unlike many conventional markers (e.g., ovalbumin, serum albumin, etc.), the Perfect Protein Markers contain no oligosaccharides that cause anomalous migration, heterogeneous "fuzzy" bands, or inaccurate size estimation. The markers are optimized for use with Coomassie blue staining, but adjusted amounts can also be used with other gel staining methods (e.g., silver staining, fluorescent dyes, etc.).

                  The Perfect Protein Markers, 15-150 kDa, include protein sizes of 15, 25, 35, 50, 75, 100, and 150 kDa. Each vial contains 400 µg protein. A 5-µl load contains 0.5 µg for each of the protein sizes, except for the 50-kDa band, which contains twice as much protein (1.0 µg).

                  The Perfect Protein Markers, 10-225 kDa, include the protein sizes listed above and two additional proteins, 10 kDa and 225 kDa, for applications requiring a broader size range. Each vial contains 500 µg protein. A 5-µl load contains 0.5 µg for each of the protein sizes, except for the 50-kDa band, which contains twice as much protein (1.0 µg).


                  The 4X SDS Sample Buffer (Cat. No. 70607), which is separately available, is a standard formulation commonly used for SDS-PAGE analysis of proteins. The solution includes DTT for complete denaturation of disulfide bonds. The buffer can be used at a final concentration of 2X for most applications.
                  Catalogue Number69149
                  Brand Family Novagen®
                  References
                  Product Information
                  FormLiquid
                  Applications
                  Biological Information
                  Physicochemical Information
                  Dimensions
                  Materials Information
                  Toxicological Information
                  Safety Information according to GHS
                  Safety Information
                  Product Usage Statements
                  Storage and Shipping Information
                  Ship Code Blue Ice Only
                  Toxicity Toxic
                  Storage -20°C
                  Avoid freeze/thaw Avoid freeze/thaw
                  Do not freeze Ok to freeze
                  Packaging Information
                  Transport Information
                  Supplemental Information
                  Specifications

                  Documentation

                  SDS

                  Title

                  Safety Data Sheet (SDS) 

                  Certificates of Analysis

                  TitleLot Number
                  69149

                  Technical Info

                  Title
                  Western Blotting Quick Reference Product Guide

                  Citations

                  Title
                • Kenji Kojima, et al. (2008) Roles of functional and structural domains of hepatocyte growth factor activator inhibitor type 1 in the inhibition of matriptase. Journal of Biological Chemistry 283, 2478-2487.
                • Athmane Teghanemt, et al. (2008) Novel roles in human MD-2 of phenylalanines 121 and 126 and tyrosine 131 in activation of toll-like receptor 4 by endotoxin. Journal of Biological Chemistry 283, 1257-1266.
                • Jim F. White, et al. (2007) Dimerization of the class A G protein-coupled neurotensin receptor NTS1 alters G protein interaction. Procedings of the National Academy of Science 104, 12199-12204.
                • Dequan Chen, et al. (2005) Surface calreticulin mediates muramyl dipeptide-induced apoptosis in RK13 cells. Journal of Biological Chemistry 280, 22425-22436.
                • Larisa Tsavaler, et al. (2001) Trp-p8, a novel prostate-specific gene, Is up-regulated in prostate cancer and other malignancies and shares high homology with transient receptor potential calcium channel proteins. Cancer Research 61, 3760-3769.
                • Kelvin Nurse, et al. (1995) Purification, cloning, and properties of the tRNA ψ55 synthase from Escherichia coli. RNA 1,.
                • Related Products & Applications

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                  Categories

                  Life Science Research > Protein Detection and Quantification > Western Blotting > Western Blotting Kits and Reagents