|Protein Concentration and Diafiltration by Tangential Flow Filtration -- An Overview|
|Biomax® Membranes -- The membrane of choice for fast processing,and exceptional chemical resistance|
|Pellicon® XL 50 Cassette and Labscale TFF System|
|Scaling Up with Tangenital Flow Filtration|
Campbell, Melissa; Coons, Elizabeth
GEN; v. 21, no. 16; Sept. 15, 2001, p. 60 2001
|Linear Scale Ultrafiltration|
van Reis, Robert, Elizabeth M. Goodrich, Christine L. Yson, Leah N. Frautschy, Stephen Dzengeleski, and Herb Lutz
Biotechnology & Bioengineering (September 1997): 737-746. 1997
|Clean-Water Permeability as a Determinant of Cleaning Efficacy in Tangential-Flow Filtration Systems|
Michaels, Stephen L.; RP016
|Validation of Tangential Flow Filtration (TFF) Systems|
Michaels, Stephen L.
Journal of Parenteral Science and Technology (Sept/Oct 1991). 1991
|What is the part number for the Gauge Kit that is used with the Pellicon XL?||Gauge Kit for Pellicon XL Device (0-60 psi):
Part number XXPXLGAGE, 1 per kit. Includes an inlet pressure gauge and t-fitting. This gauge is recommended when the Pellicon is used in the absence of a Labscale TFF system in order to ensure that the pressure on the tubing is known and not allowed to exceed it's recommended maximum.
|Can I use TFF systems to separate two proteins based on a significant difference in their respective molecular weights?||As a general rule ultrafiltration does not provide high efficiency separations of protein mixtures. However, it may be possible to effect this type of separation between two proteins that have at least 10 X difference in molecular weight and if there is an ultrafiltration membrane available with a NMWL rating between the molecular weight of the two proteins. These separations are also generally more successful with dilute protein solutions and if run at low pressure.|
|What is the sample volume range for the Pellicon II Mini system?||The Pellicon II Mini system is capable of volumes in the approximate range of 1- 50 L concentrating down to 100 mL. More or less volume can be processed depending on the individual needs of the operation, ie. process time, sensitivity of product, etc.|
|Pellicon® XL 50 Device Operating Instructions|
Pellicon® 2 Ultrafiltration CassettesDesigned with process scale-up and applications development in mind.
Learn More >>
Cogent M1 TFF SystemA state-of-the-art bench top TFF system capable of processing from 300 mL to hundreds of liters
Learn More >>
Labscale TFF SystemEasy to use for sample preparation while also providing the necessary controls for process development and scale-up work.
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Pellicon® 3 CassettesAdvanced, high performance cassettes ideally suited to today's demanding filtration processes that require more rigorous operating pressures, temperatures and caustic cleaning regimes
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|Dialysis and Filtration|
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|Biopharmaceutical Manufacturing > Downstream Processing > Ultrafiltration|
Choose between Millipore’s superior void-free ultrafiltration membranes to further specialize your cassette:
- Biomax® membranes – provides high flux and wide pH compatibility (1 – 14 range), storable in alkaline and acidic solutions, hydrophilic nature supports low protein binding.
- Ultracel® membranes – lowest protein binding, easy-to-clean, most hydrophilic, stable, reproducible flux and resistance to fouling by proteins, lipids and antifoams.
Pellicon® XL 50 Ultrafiltration Cassettes
True linear scalability with UF membranes with the same flow path and channel height as larger Pellicon® 2 cassettes. The Pellicon® XL 50 allows process development and scaling studies.
Small volume convenience yields high-end results with Millipore’s Pellicon® XL 50 Ultrafiltration Cassette. This high-performance tangential flow filter is the ideal choice for anyone contending with:
- Process Development Work (50 to 1000 mL range)
- Small Volume Processing
- Membrane Research
- Superior Performance – superior membranes and a controlled channel height capability yield reliable, consistent, predictable results.
- Void-Free Technology – guards against solute leakage.
- Self Contained – no holder required.
- Concentration, desalting, and buffer exchange – of proteins, polysaccharides, lipid solutions, viruses, colloids, cell suspensions and mammalian cells.
- Clinical trial support – quick preparation of materials
- Membrane selection – dedicated study of membrane results
- Sample preparation
- Small volume manufacturing
Choice of feed channel turbulence promoting screens to best match your process requirements:
- A Screen (tight screen)
- C Screen (coarse screen)
|Materials of Construction|
Composite Regenerated Cellulose (Ultracel®)
|Screens, Exterior Housing, Fittings/connectors and Luer Caps:||Polypropylene|
|Filtration Area||50 cm² (0.05 ft²)|
|Device Width||3.0 cm (1.2 in.)|
|Device Length||18.8 cm (7.4 in.)|
|Hold-up Volume (typical)||3.2 mL|
|Maximum Operating Pressure||5.6 bar (80 psig)|
|Optimum Tangential Flow Rate for Polarized Solutions||30 – 50 mL/min|
|Membrane pH Compatibility|
|Biomax® PES:||1 – 14|
|Ultracel® PLC:||2 – 12|
|Biocompatibility/Toxicity||All wetted parts have been tested and meet the requirements of the USP Class VI Biological Test for Plastics.|
|Extractables||Gravimetric extractables level < 7.5 mg/ device after RO water flush. USP oxidizables test negative after RO water flush.|
|Prefiltration Requirements||Prefiltration of sample using 100 μm nominal pore size filter is recommended.|
|Pellicon® XL 50 Cassette Membrane Selection Guide|
|Biomax® Membranes – Polyethersulfone||Ultracel® Membranes – Composite Regenerated Cellulose|