|Tetanus Toxoid Purification: A Case Study|
Vancetto, Mary Dalva Caparroz, Jose Marcos de Oliveira, Sally Muller Affonso Prado, Fernando Fratelli, and Hisako Gondo Higashi; Nature Biotechnology Volume 15
Nature Biotechnology Volume 15 1997
|When a Pellicon Holder is ordered, what do I receive?||The holders are supplied with a socket wrench, 3/4" sanitary clamp adapters, clamps, and gaskets. The pressure gauges need to be ordered separately by ordering catalog number XX4200075. Only one 0- 100 psi analog gauge is supplied with this number, and it is recommended to operate with two gauges, one for the feed and one for the retentate. Conversely, order the appropriate Pellicon fitting kit which contains the pressure gauges, fittings, clamps, and valves for connecting the pump to the holder.
|Are the TFF membranes autoclavable or steam sterilizable?||The membranes for Pellicon XL, Helicon UF, Helicon RO, spiral wound/prep/scale, Prostak UF and Pellicon 2 are not autoclavable or steam sterilizable. However, a chemical sanitization can be done; refer to the maintenance manual for these membranes.
Note: Prostak Open Channel with microporous Durapore membranes can be steam sterilized.
|What is the holdup volume in Pellicon Cassettes?||The holdup volume for Pellicon XL units is ~3.2 ml, Pellicon 2 modules 0.1m2(1.1ft2) is ~31ml total volume with 17ml in feed and 14ml in permeate. The Pellicon 2 Modules 0.5m2 or (5ft2) total volume of holdup is ~155mls, with 87ml in the feed and 68 int he permeate. To calculate the volume for the 2.5m2 (25ft2) multiply the volumes for 0.5m2 by five.|
|Can the Labscale TFF System accommodate more than one Pellicon XL?||PRODUCT:
Multimanifold Kit for Labscale TFF System
The Labscale Multimanifold Kit is designed to accommodate up to 3 Pellicon XL devices and mount directly onto the Labscale Reservoir. It may also be used with a peristaltic pump for those customers without a Labscale Reservoir.
It is intended to be used for either membrane selection by choosing devices with different membranes or to increase surface area by choosing devices with the same membrane.
The Labscale Multi-Manifold Kit includes 2 multi-port manifolds with assorted tubing fittings. Manifold fittings include male and female luer lock. Assorted fittings included male and female luer plugs, male to female luer lock elbows, and male luer lock to 1/8" barb.
Materials of Construction
|Cogent μScale Tangential Flow Filtration System User Guide|
|Pellicon® 3 Cassettes - Installation and User Guide|
|Pellicon® 3 Cassettes with Ultracel® Membrane Performance Guide|