539734 PTP, Yersinia enterocolitica, Recombinant, E. coli

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: YOP, Protein Tyrosine Phosphatase, Yersenia

539734
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      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      539734-2000U
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          		 Plastic ampoule 2000 u
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          Description
          Overview

          This product has been discontinued.



          Recombinant, 51 kDa catalytic domain of the Yersinia protein tyrosine phosphatase containing a C235R mutation and expressed in E. coli. Suitable for dephosphorylation of phosphotyrosine residues in proteins.
          Catalogue Number539734
          Brand Family Calbiochem®
          SynonymsYOP, Protein Tyrosine Phosphatase, Yersenia
          References
          ReferencesZhang, Z.Y., et al. 1992. J. Biol. Chem. 267, 23759.
          Gordon, J.A. 1991. Methods Enzymol. 201, 477.
          Guan, K. and Dixon, J.E. 1990. Science 249, 653.
          Product Information
          Activity≥50,000 units/ml
          Unit of DefinitionOne unit is defined as the amount of enzyme that will hydrolyze 1 nmol of <i>p</i>NPP per min at 30°C, pH 7.2.
          FormLiquid
          FormulationIn 100 mM NaCl, 50 mM HEPES, 5 mM DTT, 2 mM EDTA, 0.01% BRIJ® 35 Detergent, 50% glycerol, pH 7.0.
          Applications
          Biological Information
          Purity≥95% by SDS-PAGE
          Specific Activity≥50,000 units/mg
          Physicochemical Information
          ContaminantsProteases, serine/threonine phosphatases: none detected
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage ≤ -70°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          PTP, Yersinia enterocolitica, Recombinant, E. coli SDS

          Title

          Safety Data Sheet (SDS) 

          PTP, Yersinia enterocolitica, Recombinant, E. coli Certificates of Analysis

          TitleLot Number
          539734

          References

          Reference overview
          Zhang, Z.Y., et al. 1992. J. Biol. Chem. 267, 23759.
          Gordon, J.A. 1991. Methods Enzymol. 201, 477.
          Guan, K. and Dixon, J.E. 1990. Science 249, 653.

          Brochure

          Title
          Protein Phosphatases Technical Bulletin

          Citations

          Title
        • Meyn, M.A, et al. 2005. Molecular Pharmacology 68, 1320.
          		•
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision08-September-2008 RFH
          SynonymsYOP, Protein Tyrosine Phosphatase, Yersenia
          DescriptionRecombinant, 51 kDa catalytic domain of the Yersinia protein tyrosine phosphatase containing a C235R mutation and expressed in E. coli. Suitable for dephosphorylation of phosphotyrosine residues in proteins
          FormLiquid
          FormulationIn 100 mM NaCl, 50 mM HEPES, 5 mM DTT, 2 mM EDTA, 0.01% BRIJ® 35 Detergent, 50% glycerol, pH 7.0.
          Recommended reaction conditions50 mM Tris-HCl, pH 7.2, 150 mM NaCl, 5 mM DTT, 2.5 mM Na2EDTA, and 100 µg/ml BSA. Incubate at 30°C. 2.5 units will typically remove >95% of phosphates from tyrosine residues in 0.1 nmol of protein in 30 min.
          Purity≥95% by SDS-PAGE
          ContaminantsProteases, serine/threonine phosphatases: none detected
          Specific activity≥50,000 units/mg
          Activity≥50,000 units/ml
          Unit definitionOne unit is defined as the amount of enzyme that will hydrolyze 1 nmol of pNPP per min at 30°C, pH 7.2.
          Storage ≤ -70°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Toxicity Standard Handling
          ReferencesZhang, Z.Y., et al. 1992. J. Biol. Chem. 267, 23759.
          Gordon, J.A. 1991. Methods Enzymol. 201, 477.
          Guan, K. and Dixon, J.E. 1990. Science 249, 653.
          Citation
        • Meyn, M.A, et al. 2005. Molecular Pharmacology 68, 1320.
          		•