481409 | PTEN, His•Tag® and S•Tag™, Human Recombinant, S. frugiperda

481409
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      View Pricing & Availability
      Click To Print This Page

      Overview

      Replacement Information

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      481409-10UG
      Retrieving availability...
      Limited AvailabilityLimited Availability
      Stocked 
      Discontinued
      Limited Quantities Available
      Available
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service

          Plastic ampoule 10 μg
          Retrieving price...
          Price could not be retrieved
          Minimum Quantity needs to be mulitiple of
          Upon Order Completion More Information
          You Saved ()
           
          Request Pricing
          Description
          OverviewFull-length, recombinant, human PTEN (GenBank target symbol = PTEN, ACC No. BC_005821) fused at N-terminus to a His•Tag® and S•Tag sequences and expressed in S. frugiperda insect cells. PTEN is a tumor suppressor that is frequently found mutated in human cancers. It serves as a lipid phosphatase and negative regulator of the PI-3K/Akt pathway. Absence of functional PTEN results in constitutive activation of Akt and mTOR kinases.
          Catalogue Number481409
          Brand Family Calbiochem®
          SynonymsPhosphatase and Tensin Homolog Deleted on Chromosome 10
          References
          ReferencesHarrington, L.S., et al. 2005. Trends Biochem. Sci. 30, 35.
          Osaki, M., et al. 2004. Apoptosis 9, 667.
          Lennon, G., et al. 1996. Genomics 33, 151.
          Product Information
          Unit of DefinitionOne unit is defined as the amount of enzyme required to liberate 1 nmol phosphate from PIP₃ per min at 37°C.
          FormLiquid
          FormulationIn 150 mM NaCl, 50 mM Tris-HCl, 5 mM DTT, 1 mM EDTA, 50% glycerol, pH 7.5.
          Applications
          Biological Information
          Purity≥80% by SDS-PAGE
          Specific Activity≥75 U/mg protein
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage ≤ -70°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          481409

          References

          Reference overview
          Harrington, L.S., et al. 2005. Trends Biochem. Sci. 30, 35.
          Osaki, M., et al. 2004. Apoptosis 9, 667.
          Lennon, G., et al. 1996. Genomics 33, 151.

          Brochure

          Title
          Calbiochem Biologics 32.2
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision12-June-2008 RFH
          SynonymsPhosphatase and Tensin Homolog Deleted on Chromosome 10
          DescriptionFull-length, recombinant, human PTEN (GenBank target symbol = PTEN, ACC No. BC_005821) fused at N-terminus to a His•Tag® and S•Tag sequences and expressed in S. frugiperda insect cells. PTEN is a tumor suppressor that is frequently found mutated in human cancers. It serves as a lipid phosphatase and negative regulator of the PI-3K/Akt pathway. Absence of functional PTEN results in constitutive activation of Akt and mTOR kinases.
          FormLiquid
          FormulationIn 150 mM NaCl, 50 mM Tris-HCl, 5 mM DTT, 1 mM EDTA, 50% glycerol, pH 7.5.
          Concentration Label Please refer to vial label for lot-specific concentration
          Recommended reaction conditions100 mM Tris-HCl, 10 mM DTT, pH 8.0, 25 µg/ml PTEN, 37°C.
          Purity≥80% by SDS-PAGE
          Specific activity≥75 U/mg protein
          Unit definitionOne unit is defined as the amount of enzyme required to liberate 1 nmol phosphate from PIP₃ per min at 37°C.
          Storage ≤ -70°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Toxicity Standard Handling
          ReferencesHarrington, L.S., et al. 2005. Trends Biochem. Sci. 30, 35.
          Osaki, M., et al. 2004. Apoptosis 9, 667.
          Lennon, G., et al. 1996. Genomics 33, 151.