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14-334 | PRAK Protein, active, 15 µg

15 µg  Also available in 250μg size (2x125μg) - Call for pricing and availability. Please reference catalog number 14-334M when ordering the 250μg size.
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      Replacement Information
      Catalogue Number14-334
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionPRAK Protein, active, 15 µg
      OverviewN-terminal His6-tagged recombinant, human, full-length PRAK
      Product Information
      ApplicationActive, N-terminal His6-tagged recombinant, human, full-length PRAK, for use in Kinase Assays.
      Key Applications
      • Kinase Assay
      Biological Information
      Sourceexpressed by baculovirus in Sf21 insect cells
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the serine/threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. In vitro, this kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites. Two alternately spliced transcript variants of this gene encoding distinct isoforms have been reported.
      Gene Symbol
      • MAPKAPK5
      • PRAK
      Protein TargetPRAK
      Purification MethodNi2+/NTA-agarose
      Target Sub-FamilyCAMK
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q8IW41 # Mediates stress-induced small heat shock protein 27 phosphorylation.
      SIZE: 473 amino acids; 54220 Da
      SUBUNIT: Interacts with SQSTM1.
      SUBCELLULAR LOCATION: Cytoplasm (Probable). Nucleus. Note=Also observed in the nucleus.
      TISSUE SPECIFICITY: Expressed ubiquitously.
      PTM: Phosphorylated on Thr-182; which is the regulatory phosphorylation site and is located on the T-loop/loop 12.
      SIMILARITY: SwissProt: Q8IW41 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. & Contains 1 protein kinase domain.
      Molecular Weight55kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by phosphorylation of PRAK Substrate Peptide
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions6 months at -70C
      Packaging Information
      Material Size15 µg
      Material PackageAlso available in 250μg size (2x125μg) - Call for pricing and availability. Please reference catalog number 14-334M when ordering the 250μg size.
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      PRAK, active - 1599660-A 1599660-A
      PRAK, active - 1599660-B 1599660-B
      PRAK, active - 25789U-B 25789U-B


      Reference overviewApplicationPub Med ID
      Specificity and mechanism of action of some commonly used protein kinase inhibitors.
      Davies, S P, et al.
      Biochem. J., 351: 95-105 (2000) 2000

      Show Abstract
      Kinase Assay10998351 10998351
      PRAK, a novel protein kinase regulated by the p38 MAP kinase.
      New, L, et al.
      EMBO J., 17: 3372-84 (1998) 1998

      Show Abstract
      9628874 9628874

      Related Products & Applications

      Alternative Packsize

      Catalog Number Description  
      14-334M PRAK Protein, active, 250 µg Show Pricing & Availability

      Product Families


      Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases
      Life Science Research > Proteins and Enzymes > Purified Kinases