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60-065 | PP2A Cα subunit siRNA/siAb™ Assay Kit

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      Replacement Information
      Catalogue Number60-065
      Brand Family Upstate
      Trade Name
      • siRNA/siAB
      • Upstate
      DescriptionPP2A Cα subunit siRNA/siAb™ Assay Kit
      OverviewsiRNA/siAb™ Assay Kits provide convenience and economy by combining into one format products that are guaranteed to knock-down the targeted mRNA and detect the level of the targeted protein. The kit's siRNA SMARTpool®s and antibodies have been extensively validated and together with the included positive and negative controls, they make data generation easier and more trusted. Components available separately. Capacity dependent on choice of plate format, see COA.
      Product Information
      • Anti-PP2A, C subunit, clone 7A6 (Cat.# 05-545)
      • PP2A Cα subunit SMARTpool® siRNA reagent (Cat.# M-003598)
      • A431 cell lysate
      • D-001206
      • 5X Universal Buffer no longer supplied (See Application Notes for details.)
      Key Applications
      • RNAi/siRNA/Gene Knockdown
      • Western Blotting
      Application NotesTo assist researchers in addressing and minimizing potentially confounding issues associated with siRNA-dependent gene silencing.; 5X Universal Buffer is no longer supplied with this kit, however, it can be made using the recipe found here.
      Biological Information
      Species Reactivity
      • Human
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit.
      Gene Symbol
      • PPP2CA
      • PP2CA
      • PP2A-alpha
      • PP2Ac
      • RP-C
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P67775 # PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Can dephosphorylate SV40 large T antigen and p53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen.
      COFACTOR: Binds 1 iron ion per subunit (By similarity). & Binds 1 manganese ion per subunit (By similarity).
      SIZE: 309 amino acids; 35594 Da
      SUBUNIT: PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules.
      PTM: Reversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly. It varies during the cell cycle. & Phosphorylation of either threonine (by autophosphorylation- activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.
      SIMILARITY: SwissProt: P67775 ## Belongs to the PPP phosphatase family. PP-1 subfamily.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Availability by Geography
      • This product is not available for sale in Australia, New Zealand, Singapore, Indonesia, Thailand, The Phillippines, Malaysia, Vietnam, Japan, Korea, and China.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size1 kit
      Transport Information
      Supplemental Information

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      Kit Component

      Catalog Number Description  
      05-545 Anti-PP2A Antibody, C subunit, clone 7A6 Show Pricing & Availability

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