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17-139 | PKC Assay Kit

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      Replacement Information

      Key Specifications Table

      Key ApplicationsAnalytes AvailableDetection Methods
      KA PKC Radioactive
      Catalogue Number17-139
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionPKC Assay Kit
      Product Information
      • PKA/CaMK Inhibitor Cocktail (Cat.# 20-132)
      • PKC Substrate Cocktail (Cat.# 20-131)
      • Assay Dilution Buffer II (ADBII) (Cat.# 20-111)
      • PKC Lipid Activator (Cat.# 20-133)
      • Magnesium/ATP Cocktail (Cat.# 20-113)
      • P81 Phosphocellulose Squares (Cat.# 20-134)
      Detection methodRadioactive
      ApplicationThe radioactive PKC Assay Kit is designed to measure the phosphotransferase activity of protein kinase C (PKC) in immunoprecipitates & column fractions.
      Key Applications
      • Kinase Assay
      Biological Information
      Analytes Available
      • PKC
      Entrez Gene Number
      Entrez Gene SummaryProtein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.
      Gene Symbol
      • PRKCA
      • PKC-A
      • PKCA
      • MGC129901
      • AAG6
      • MGC129900
      • PRKACA
      • PKC-alpha
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P17252 # PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
      COFACTOR: Binds 3 calcium ions per subunit. The ions are bound to the C2 domain (By similarity).
      SIZE: 672 amino acids; 76764 Da
      SUBUNIT: Interacts with CENTA1, CSPG4 and PRKCABP. Binds to SDPR in the presence of phosphatidylserine.
      SIMILARITY: SwissProt: P17252 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 C2 domain. & Contains 2 phorbol-ester/DAG-type zinc fingers. & Contains 1 protein kinase domain.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by phosphorylation of PKC substrate peptide.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size1 kit
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      PKC Assay Kit - 2120316 2120316
      PKC Assay Kit - 2121976 2121976
      PKC Assay Kit - 2382139 2382139
      PKC Assay Kit - 2386491 2386491
      PKC Assay Kit - 2436383 2436383
      PKC Assay Kit - 16218 16218
      PKC Assay Kit - 16284 16284
      PKC Assay Kit - 17198 17198
      PKC Assay Kit - 17326 17326
      PKC Assay Kit - 17790 17790
      PKC Assay Kit - 18048 18048
      PKC Assay Kit - 18774 18774
      PKC Assay Kit - 19356 19356
      PKC Assay Kit - 19716 19716
      PKC Assay Kit - 2059475 2059475
      PKC Assay Kit - 20636 20636
      PKC Assay Kit - 20981 20981
      PKC Assay Kit - 2153155 2153155
      PKC Assay Kit - 21907 21907
      PKC Assay Kit - 2266828 2266828
      PKC Assay Kit - 23263 23263
      PKC Assay Kit - 25013 25013
      PKC Assay Kit - 2506471 2506471
      PKC Assay Kit - 27376 27376
      PKC Assay Kit - 31611 31611
      PKC Assay Kit - DAM1437088 DAM1437088
      PKC Assay Kit - DAM1682204 DAM1682204
      PKC Assay Kit - DAM1734821 DAM1734821
      PKC Assay Kit - JBC1897217 JBC1897217
      PKC Assay Kit - JBC1920640 JBC1920640
      PKC Assay Kit -2453220 2453220
      PKC Assay Kit -2601695 2601695


      Reference overviewApplicationPub Med ID
      Human 100-kDa homologous DNA-pairing protein is the splicing factor PSF and promotes DNA strand invasion
      Akhmedov, A. T. and Lopez, B. S.
      Nucleic Acids Res, 28:3022-30 (2000) 2000

      Kinase Assay10931916 10931916
      Selective inhibition of protein kinase C isozymes by Fas ligation
      Chen, C. Y. and Faller, D. V.
      J Biol Chem, 274:15320-8 (1999) 1999

      Enzyme Assay10336417 10336417

      Related Products & Applications

      Kit Component

      Catalog Number Description  
      20-134 P81 Phosphocellulose Squares Show Pricing & Availability
      20-111 Assay Dilution Buffer II (ADBII) Show Pricing & Availability
      20-113 Magnesium/ATP Cocktail Show Pricing & Availability
      20-131 PKC Substrate Cocktail Show Pricing & Availability
      20-132 PKA/CaMK Inhibitor Cocktail Show Pricing & Availability
      20-133 PKC Lipid Activator Show Pricing & Availability


      Life Science Research > Protein Detection and Quantification > Activity Assays > Kinase Assays