539481 PKA, Catalytic Subunit, Mouse, Recombinant, E. coli

539481
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      Overview

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      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      539481-100KU
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          Plastic ampoule 100 ku
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          Description
          OverviewRecombinant, mouse PKA catalytic subunit expressed in E. coli. Regulates a number of cellular functions including gene expression, carbohydrate metabolism, Ca2+ homeostasis, and ion channel function. Suitable for studying the phosphorylation of purified substrates and for use with cell extracts. Unlike the holoenzyme, the catalytic subunit does not require cAMP for activation.
          Catalogue Number539481
          Brand Family Calbiochem®
          SynonymscAMP-Dependent Protein Kinase, Catalytic Subunit, Protein Kinase A, Catalytic Subunit
          References
          ReferencesGrant, B.D., and Adams, J.A. 1996. Biochemistry 35, 2022.
          Lee, S-L. and Steinberg, R.A. 1996. J. Biol. Chem. 271, 16553.
          Adams, J.A., et al. 1995. Biochemistry 34, 2447.
          Bosma, M.M., et al. 1993. J. Neurosci. 13, 5242.
          Brindle, P., et al. 1993. Nature 364, 821.
          Hajnoczky, G., et al. 1993. Biochem. J. 293, 413.
          Sculptoreanu, A., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 10135.
          Wang, L-Y., et al. 1993. Science 259, 1173.
          Slice, L.W., and Taylor, S.S. 1989. J. Biol. Chem. 264, 20940.
          Uhler, M.D., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 1300.
          Walsh, D.A., et al. 1968. J. Biol. Chem. 243, 3763.
          Product Information
          Activity≥2.5 x 10⁶ U/ml
          Unit of DefinitionOne unit is defined as the amount of enzyme that will transfer 1.0 pmol phosphate to Kemptide (LRRASLG; <a href ="/Products/ProductDisplay.asp→catNO=05-23-4900">Cat. No. 05-23-4900</a>) per min at 30°C, pH 7.5.
          EC number2.7.1.37
          FormLiquid
          FormulationIn 50 mM NaCl, 20 mM Tris-HCl, 1 mM EDTA, 2 mM DTT, 50% glycerol, pH 7.5.
          Applications
          Biological Information
          Physicochemical Information
          ContaminantsPhosphatases, proteases: none detected
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          PKA, Catalytic Subunit, Mouse, Recombinant, E. coli SDS

          Title

          Safety Data Sheet (SDS) 

          PKA, Catalytic Subunit, Mouse, Recombinant, E. coli Certificates of Analysis

          TitleLot Number
          539481

          References

          Reference overview
          Grant, B.D., and Adams, J.A. 1996. Biochemistry 35, 2022.
          Lee, S-L. and Steinberg, R.A. 1996. J. Biol. Chem. 271, 16553.
          Adams, J.A., et al. 1995. Biochemistry 34, 2447.
          Bosma, M.M., et al. 1993. J. Neurosci. 13, 5242.
          Brindle, P., et al. 1993. Nature 364, 821.
          Hajnoczky, G., et al. 1993. Biochem. J. 293, 413.
          Sculptoreanu, A., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 10135.
          Wang, L-Y., et al. 1993. Science 259, 1173.
          Slice, L.W., and Taylor, S.S. 1989. J. Biol. Chem. 264, 20940.
          Uhler, M.D., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 1300.
          Walsh, D.A., et al. 1968. J. Biol. Chem. 243, 3763.

          Citations

          Title
        • Gerald Wilson, et al. (2003) Phosphorylation of p40AUF1 regulates binding to A + U-rich mRNA-destabilizing elements and protein-induced changes in ribonucleoprotein structure.. Journal of Biological Chemistry 278, (33039- 33048).
        • Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision02-January-2008 JSW
          SynonymscAMP-Dependent Protein Kinase, Catalytic Subunit, Protein Kinase A, Catalytic Subunit
          DescriptionRecombinant, mouse PKA catalytic subunit expressed in E. coli. The catalytic subunit of protein kinase A that combines with the regulatory subunit, in the absence of cAMP, to form the inactive PKA holoenzyme. Unlike the holoenzyme, the catalytic subunit does not require cAMP for activation. PKA-Cα is reported to potentiate IP3-induced Ca2+ mobilization in isolated hepatocytes. Suitable for studies of PKA phosphorylation in vitro with purified substrates or following the introduction into intact cells or cell extracts.
          FormLiquid
          FormulationIn 50 mM NaCl, 20 mM Tris-HCl, 1 mM EDTA, 2 mM DTT, 50% glycerol, pH 7.5.
          Recommended reaction conditions50 mM Tris-HCl, pH 7.5, 10 mM MgCl2, 200 µM ATP, and γ-labeled-32P-ATP to a final specific activity of 200 μCi/μmol. Incubate at 30°C.
          EC number2.7.1.37
          ContaminantsPhosphatases, proteases: none detected
          Activity≥2.5 x 10⁶ U/ml
          Unit definitionOne unit is defined as the amount of enzyme that will transfer 1.0 pmol phosphate to Kemptide (LRRASLG; Cat. No. 05-23-4900) per min at 30°C, pH 7.5.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesGrant, B.D., and Adams, J.A. 1996. Biochemistry 35, 2022.
          Lee, S-L. and Steinberg, R.A. 1996. J. Biol. Chem. 271, 16553.
          Adams, J.A., et al. 1995. Biochemistry 34, 2447.
          Bosma, M.M., et al. 1993. J. Neurosci. 13, 5242.
          Brindle, P., et al. 1993. Nature 364, 821.
          Hajnoczky, G., et al. 1993. Biochem. J. 293, 413.
          Sculptoreanu, A., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 10135.
          Wang, L-Y., et al. 1993. Science 259, 1173.
          Slice, L.W., and Taylor, S.S. 1989. J. Biol. Chem. 264, 20940.
          Uhler, M.D., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 1300.
          Walsh, D.A., et al. 1968. J. Biol. Chem. 243, 3763.
          Citation
        • Gerald Wilson, et al. (2003) Phosphorylation of p40AUF1 regulates binding to A + U-rich mRNA-destabilizing elements and protein-induced changes in ribonucleoprotein structure.. Journal of Biological Chemistry 278, (33039- 33048).