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14-602-D | PI3 kinase (p110α/p85α)

20 μg  
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      Replacement Information
      Catalogue Number14-602-D
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionPI3 kinase (p110α/p85α)
      OverviewComplex of N-terminal FLAG tagged recombinant full-length human p110α, and recombinant full length, human p85α (no tag), coexpressed in a Baculovirus infected Sf9 cell
      expression system
      Product Information
      Presentation25 mM Tris, pH 8.0, 69 mM NaCl, 1.35 mM KCl, 0.025% Tween-20, 3 mM DTT, and 50% glycerol
      ApplicationComplex of N-terminal FLAG tagged recombinant full-length human p110α, and recombinant full length, human p85α (no tag), coexpressed in a Baculovirus infected Sf9 cell
      expression system, for use in enzyme kinetics, screening inhibitors, and selectivity profiling.
      Key Applications
      • Kinase Assay
      Biological Information
      Concentration0.55 mg/ml
      SourceSf9 insect cells
      Specific Activity1680 pmol/min/μg
      Entrez Gene Number
      Entrez Gene SummaryPhosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers.
      Gene Symbol
      • PIK3CA
      • PI3K
      • MGC142161
      • p110-alpha
      • MGC142163
      Target Sub-FamilyPI3K/PI4K
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P42336 # Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2.
      SIZE: 1068 amino acids; 124284 Da
      SUBUNIT: Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunit. Binds to IRS1 in nuclear extracts. Interacts with RUFY3 (By similarity).
      DISEASE: SwissProt: P42336 # Defects in PIK3CA are associated with colorectal cancer (CRC) [MIM:114500]. & Defects in PIK3CA are associated with breast cancer [MIM:114480]. & Defects in PIK3CA are associated with epithelial ovarian cancer (EOC) [MIM:604370].
      SIMILARITY: SwissProt: P42336 ## Belongs to the PI3/PI4-kinase family. & Contains 1 C2 domain. & Contains 1 PI3K/PI4K domain.
      Molecular Weight125 kDa (p110α), 84 kDa (p85α)
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Kinase assay: A 50 μl kinase reaction is conducted in a buffer containing 40 mM Tris-HCl (pH 7.5), 20 mM MgCl2, 100 μM ATP, 200 μM L-α -phosphatidylinositol/ L-α-phosphatidylserine (PI/PS) (Avanti, Alabaster, AL), and 20-100 ng enzyme for 30 min at 30°C. ATP reduction is detected using ADP-Glo™ Kinase Assay Platform
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for > 6 month at –80°C from date of receipt.
      Packaging Information
      Material Size20 μg
      Transport Information
      Supplemental Information