17-493 | PI3 Kinase Activity/Inhibitor ELISA

17-493
1 plate  Kit capacity: 96 wells
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      Overview

      Replacement Information

      Key Specifications Table

      Key ApplicationsAnalytes AvailableDetection Methods
      KA PI 3-Kinase Colorimetric, HTRF
      Description
      Catalogue Number17-493
      DescriptionPI3 Kinase Activity/Inhibitor ELISA
      References
      Product Information
      Components
      • Glutathione-Coated Plate, 96-well strip format
      • 20% Tween® 20
      • 20X TBS Wash
      • Substrate TMB
      • Stop Buffer
      • SA-HRP Detection Reagent
      • GRP1-GST
      • Kinase Reaction Buffer, 5X
      • Wortmannin
      • PIP2
      • Biotinylated-PIP3
      • PI3K, p110α
      • PI3K, p110β
      • PI3K, p110δ
      • PI3K, p120γ
      Detection methodColorimetric HTRF
      Applications
      ApplicationThis PI3 Kinase Activity/Inhibitor ELISA is used to measure & quantify PI 3-Kinase levels.
      Key Applications
      • Kinase Assay
      Biological Information
      Analytes Available
      • PI 3-Kinase
      Entrez Gene Number
      Entrez Gene SummaryPhosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers.
      Gene Symbol
      • p110-alpha
      • MGC142161
      • PI3K
      • MGC142163
      • PIK3CA
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P42336 # Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2.
      SIZE: 1068 amino acids; 124284 Da
      SUBUNIT: Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunit. Binds to IRS1 in nuclear extracts. Interacts with RUFY3 (By similarity).
      DISEASE: SwissProt: P42336 # Defects in PIK3CA are associated with colorectal cancer (CRC) [MIM:114500]. & Defects in PIK3CA are associated with breast cancer [MIM:114480]. & Defects in PIK3CA are associated with epithelial ovarian cancer (EOC) [MIM:604370].
      SIMILARITY: SwissProt: P42336 ## Belongs to the PI3/PI4-kinase family. & Contains 1 C2 domain. & Contains 1 PI3K/PI4K domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size1 plate
      Material PackageKit capacity: 96 wells
      Transport Information
      Supplemental Information
      Specifications

      Related Products & Applications

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      Categories

      Life Science Research > Protein Detection and Quantification > Activity Assays > Kinase Assays