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14-452 | PDK1 Protein, active, 10 µg

10 µg  Also available in 250 µg size --call for pricing and availability and reference catalog number 14-452M when ordering the 250 µg size.
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      Replacement Information
      Catalogue Number14-452
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionPDK1 Protein, active, 10 µg
      OverviewN-terminal, His6-tagged, recombinant human PDK1, amino acids 52-end.
      Product Information
      Presentation50mM Tris-HCl, pH 7.5, 0.15M NaCl, 0.1mM EGTA, 0.03% Brij-35, 0.1% 2-mercaptoethanol, 1mM benzamidine, 200μM PMSF and 5% glycerol
      ApplicationActive, N-terminal, His6-tagged, recombinant human PDK1, amino acids 52-end. For use in Kinase Assays.
      Key Applications
      • Kinase Assay
      Biological Information
      SourceHuman PDK1 expressed by baculovirus in Sf21 insect cells
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Gene Symbol
      • PDPK1
      • MGC20087
      • PDK1
      • PkB-like
      • PRO0461
      • MGC35290
      • hPDK1
      Protein TargetPDK1
      Purification MethodNi2+/NTA-agarose
      Target Sub-FamilyAGC
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: O15530 # Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.
      SIZE: 556 amino acids; 63152 Da
      SUBCELLULAR LOCATION: Cytoplasm. Membrane; Peripheral membrane protein. Note=Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.
      TISSUE SPECIFICITY: Appears to be expressed ubiquitously.
      PTM: Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation.
      SIMILARITY: SwissProt: O15530 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily. & Contains 1 PH domain. & Contains 1 protein kinase domain.
      Molecular Weight59kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by phosphorylation of PDKtide
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions6 months at -70°C
      Packaging Information
      Material Size10 µg
      Material PackageAlso available in 250 µg size --call for pricing and availability and reference catalog number 14-452M when ordering the 250 µg size.
      Transport Information
      Supplemental Information

      Related Products & Applications

      Alternative Packsize

      Catalog Number Description  
      14-452M PDK1 Protein, active, 250 µg Show Pricing & Availability

      Product Families


      Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases
      Life Science Research > Proteins and Enzymes > Purified Kinases