521270 PDK1, His•Tag®, Human, Recombinant, S. frugiperda

521270
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      Overview

      Replacement Information

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      521270-5UG
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      Stocked 
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          Plastic ampoule 5 μg
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          Description
          OverviewFull length, recombinant, human PDK1 fused to a His•Tag® sequence and expressed in S. frugiperda insect cells. PDK1 is a serine/threonine protein kinase that is localized to the plasma membrane. PDK1 activates conventional protein kinase C (PKC) as well as PKCζ. PDK1 also phosphorylates Akt and plays a role in insulin signaling.
          Catalogue Number521270
          Brand Family Calbiochem®
          Synonyms3-Phosphoinositide-Dependent Protein Kinase-1
          References
          ReferencesScott, M.T., et al. 2002. EMBO J. 21, 6771.
          Biondi, R.M., et al. 2000. EMBO J. 19, 979.
          Alessi, D.R., et al. 1997. Curr. Biol. 7, 776.
          Product Information
          Unit of DefinitionOne unit is defined as the amount of enzyme that will transfer 1 nmol of phosphate to PDK1 substrate per min at 30°C, pH 7.5.
          EC number2.7.10
          FormLiquid
          FormulationIn 100 mM NaCl, 50 mM Tris-HCl, 5 mM DTT, 500 µM EDTA, 500 µM EGTA, 20% glycerol, 0.05% TRITON® X-100 Detergent, pH 7.5.
          Applications
          Biological Information
          Purity≥90% by SDS-PAGE
          Specific Activity≥75 units/mg protein
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage ≤ -70°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          521270

          References

          Reference overview
          Scott, M.T., et al. 2002. EMBO J. 21, 6771.
          Biondi, R.M., et al. 2000. EMBO J. 19, 979.
          Alessi, D.R., et al. 1997. Curr. Biol. 7, 776.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision12-August-2014 JSW
          Synonyms3-Phosphoinositide-Dependent Protein Kinase-1
          DescriptionFull length, recombinant, human PDK1 fused to a His•Tag® sequence and expressed in S. frugiperda insect cells. PDK1 is a serine/threonine protein kinase that is localized to the plasma membrane. PDK1 activates conventional protein kinase C (PKC) as well as PKCζ. PDK1 also phosphorylates Akt and plays a role in insulin signaling.
          FormLiquid
          FormulationIn 100 mM NaCl, 50 mM Tris-HCl, 5 mM DTT, 500 µM EDTA, 500 µM EGTA, 20% glycerol, 0.05% TRITON® X-100 Detergent, pH 7.5.
          Concentration Label Please refer to vial label for lot-specific concentration
          EC number2.7.10
          Purity≥90% by SDS-PAGE
          Specific activity≥75 units/mg protein
          Unit definitionOne unit is defined as the amount of enzyme that will transfer 1 nmol of phosphate to PDK1 substrate per min at 30°C, pH 7.5.
          Storage ≤ -70°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Toxicity Standard Handling
          ReferencesScott, M.T., et al. 2002. EMBO J. 21, 6771.
          Biondi, R.M., et al. 2000. EMBO J. 19, 979.
          Alessi, D.R., et al. 1997. Curr. Biol. 7, 776.